The relations of prostatic cancer growth activity with metastasis suppresser gene, androgen receptor gene mutation and vascular endothelial growth factor

根据上一年的性能报告，对石蜡包埋制剂中的Ki-67进行了化学分析研究。从106例未经治疗的前列腺癌患者中获得穿刺活检标本。在组织制备物中评估至少500个癌细胞。计算Ki-67阳性率并用作Ki-67标记指数。上述检查由一名医生进行。在106例患者中，118例患者的癌细胞少于500个，因此这些病例从平均Ki-67标记指数的计算中排除。Ki-67标记指数平均为13.6%，中位数为8.1%。关于Ki-67标记指数与肿瘤恶性程度之间的关系，Gleason评分较高的组中Ki-67标记指数较高，表明Ki-67标记指数与恶性程度高度相关。将患者分为Ki-67标记指数为8.1%或更高， ...更多信息 Ki-67标记指数低于8.1%的病例，观察Ki-67标记指数与生存率的关系。结果，Ki-67标记指数为8.1%或更高的组的生存期明显缩短。基于这些发现，证实Ki-67标记指数反映了前列腺癌的恶性程度和预后，因此，是前列腺癌生长活性的适当参数。与此相反，血管内皮生长因子免疫组化染色的适当条件的建立并不成功，目前正在研究中。关于使用冷冻样品的KAI 1转移抑制基因编码的蛋白质的免疫组织化学研究和雄激素受体基因分析，由于冷冻样品的数量不足以进行任何一种程序，因此无法建立适当的条件，并且正在研究两种程序的条件，包括额外的样品收集。因此，在本研究中不能研究前列腺癌生长活性与血管内皮生长因子或KAI 1转移抑制基因表达的关系。少

Ki-67 was immunohistochemically investigated in paraffin-embedded preparations according to the performance report for the previous year. Needle biopsy specimens were obtained from 106 untreated patients with prostatic cancer. At least 500 cancer cells were evaluated in a tissue preparation. The Ki-67- positivity rate was calculated and used as the Ki-67 labeling index. The above examination was performed by one physician. Less than 500 cancer cells were evaluated in 118 of 106 patients, thus these cases were excinded from calculation of the mean Ki-67 labeling index. The mean Ki-67 labeling index of 88 cases was 13.6% and the median value was 8.1%. Regarding the relationship between the Ki-67 labeling index and the degree of tumor- malignancy, the Ki-67 labeling index was higher in groups with a higher Gleason score, showing that the Ki-67 labeling index highly correlated with the degree of malignancy. The patients were divided into these with 8.1% or higher Ki-67 labeling index and t … More he others with Ki-67 labeling index lower than 8.1%, then the relationship between the Ki-67 labeling index and survival rate was investigated. As a result, the survival period was significantly shorter in the group with 8.1% or higher Ki-67 labeling index. Based on these findings, it was confirmed that the Ki-67 labeling index reflects the degree of malignancy and prognosis in prostatic cancer, and thus, in an appropriate parameter of prostatic cancer growth activity. In contrast, estabhishrnent of appropriate conditions for immunohistochemical staining of vascular endothelial growth factor was not successful and is under investigation. Regarding immunohistochemical investigation of the protein encoded by KAI 1 metastasis suppresser gene and androgen receplor gene analysis using frozen samples, since the number of frozen samples was not sufficient for either procedure, appropriate conditions could not be established, and the conditions for both procedures are under investigation, including additional sample collection. ln consequence, the relation of prostatic cancer growth activity with either vascular endothelial growth factor or KAI 1 metastasis suppresser gene expression could not be investigated in this study. Less