Study of oviductal spontaneous contraction in relation to reciprocal controlling mechanism between fallopian tube and gamete or embryo.

输卵管自发收缩与输卵管与配子或胚胎相互控制机制的研究。

• 批准号: 03670791
• 负责人: NOZAKI Masahiro
• 金额: $ 1.41万
• 依托单位: Kyusyu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670791/
• 关键词: Phospholipase A_2 activity; Rabbit oviducts; Epithelial cells; Estrogens; Progesterone; プロゲステロン; 家兎卵管; ホスホリパ-ゼA_2活性

Phospholipase A_2 (EC 3.1.1.4) activity of the epithelium from rabbit oviducts was compared in the existence of various, ovarian steroids to assess how they could modulate prostaglandins (PG_S) biosynthesis in the oviduct. At least four kinds of phospholipass A_2 (PLA_2) activities with respect to calcium dependency and pH requirement were observed. These were two calcium-depredent, pH optima of 7.5 and 8.5 activities, and two calcium-independent, pH optima of 4.0 and 8.0 activities. One of those activities, a calcium-dependent and alkaline active PLA_2 activity of the epithelial cells was then compared between the ampullary portion and the isthmic portion of the oviducts. The activity was significantly higher in the ampullary epithelium the in the isthmic epithelium (223.2 (〕SY.+-.〔) 57.2 ir 103.8 (〕SY.+-.〔) 32.3 pmol/min/mg. p<0.05). Therefore PLA_2 activity of the ampullary epithelium from rabbit oviducts was compared in the existence of various ovarian steroids. The PLA_2 activity of the cells from ovariectomized rabbits (control) was 190.8 (〕SY.+-.〔) 9.8 pmol/min/mg. The PLA_2 activity of the cells from progesterone-treated rabbits was 156.0 (〕SY.+-.〔) 41.8 pmol/min/mg and was not significant compared to the control activity. However, the PLA_2 activity of the cells from the estrogen-treated rabbits was 233.5 (〕SY.+-.〔) 29.0 pmol/min/mg. which was significantly higher than the control activity (p<0.05). On the other hand, the PLA_2 activity of the cells from progesterone-treated rabbits after being primed with estrogen was 116.3 (〕SY.+-.〔) 25.9 pmol/min/mg. which was significantly lower than the control activity (p<0.01). These results suggest that the changes in PLA_2 activity by ovarian steroids could regulate the local production of PG_S which plays a role in both smooth muscle contractility and celiray activity.

在各种卵巢类固醇的存在下比较兔输卵管上皮的磷脂酶 A_2 (EC 3.1.1.4) 活性，以评估它们如何调节输卵管中前列腺素 (PG_S) 的生物合成。观察到至少四种与钙依赖性和 pH 要求有关的磷脂通 A_2 (PLA_2) 活性。它们是两种缺钙、最适 pH 值 7.5 和 8.5 的活性，以及​​两种不依赖钙、最适 pH 4.0 和 8.0 的活性。然后比较输卵管壶腹部分和峡部部分之间的这些活性之一，即上皮细胞的钙依赖性和碱性活性PLA_2活性。壶腹上皮的活性显着高于峡部上皮（223.2 (〕SY.+-.〔) 57.2 ir 103.8 (〕SY.+-.〔) 32.3 pmol/min/mg. p<0.05）。因此，比较了不同卵巢类固醇存在下兔输卵管壶腹上皮的PLA_2活性。去卵巢兔（对照）的细胞的PLA_2活性为190.8（〕SY.+-.〔）9.8 pmol/min/mg。黄体酮处理的兔细胞的PLA_2活性为156.0(〕SY.+-.〔)41.8 pmol/min/mg，与对照活性相比不显着。然而，雌激素处理的兔子细胞的PLA_2活性为233.5(〕SY.+-.〔)29.0 pmol/min/mg。其活性显着高于对照活性（p<0.05）。另一方面，用雌激素引发后，来自黄体酮处理的兔子的细胞的PLA_2活性为116.3(〕SY.+-.〔)25.9 pmol/min/mg。其活性显着低于对照活性（p<0.01）。这些结果表明，卵巢类固醇引起的 PLA_2 活性的变化可以调节 PG_S 的局部产生，PG_S 在平滑肌收缩性和细胞活性中发挥作用。

项目成果

T.Morishita: "Regional differences of phospholipase A_2 activity in the rabbit oviductal epithelium." Prostaglandins Leukotrienes and Essendial Fatty Acids. 47. 199-202 (1992)

T.Morishita：“兔输卵管上皮中磷脂酶 A_2 活性的区域差异。”