The study of experimental autoimmune uveoretinitis in Brown Norway rats treated by renal cryosurgery

与Lewis大鼠相比，100%接种了光感受器间类视黄酮结合蛋白（IRBP）和完全佐剂的动物发生实验性自身免疫性葡萄膜视网膜炎（EAU）， Brown Norway （BN）大鼠相对耐药。然而，接受肾冷冻治疗的BN大鼠有很高的EAU发展率。我们着手确定致病过程和共同抗原参与这种特殊类型的EAU。(1)以抗IRBP单克隆抗体为配体，亲和层析法提取纯化的IRBP。这种新方法使我们能够避免传统的Con A柱的问题，其中结果受到Con A本身的污染的影响。(2)为了阐明EAU的致病过程，我们研究了接种IRBP后分离的脾淋巴细胞对IRBP的免疫应答。在接种后早期分离的淋巴细胞表现出增殖反应，抗irbp抗体在接种后第7天鉴定出更多的dy。此外，还同时鉴定出抗肾和RPE的抗体。因此，我们认为体液免疫和细胞免疫对这类EAU的发展很重要。(3)血视网膜屏障（BRB）的破坏是EAU形成的必要条件，但Lewis大鼠正常接种后产生的EAU与BN大鼠肾细胞治疗后产生的EAU存在差异。虽然BN大鼠BRB的损伤取决于针对RPE的抗体的发展，但在Lewis大鼠中，粘附分子ICAM-1和LFA-1对BRB的破坏很重要。(4)发现了一种针对眼组织的血清抗体，这种抗体是对肾抗原暴露的反应。(5)然而，使用抗irbp单克隆抗体作为亲和层析的配体，我们无法识别常见的肾脏和眼部组织抗原。我们计划使用上述第4条中描述的抗体进行进一步的研究。少

In comparison to the Lewis rat, in which 100% of animals innoculated with interphotoreceptor retinoid-binding protein (IRBP) and complete adjuvant develop experimental autoimmune uveoretinitis (EAU), the Brown Norway (BN) rat is relatively resistant. However, BN rats who have undergone renal cryotherapy have a high rate of EAU development. We set out to identify the pathogenic process and the common antigen involved in this particular type of EAU. (1) Using an anti-IRBP monoclonal antibody as a ligand, we extracted purified IRBP by affinity chromatography. This new method allowed us to avoid the problem of conventional Con A columns, in which the results are influenced by contamination by Con A itself. (2) In order to elucidate the pathogenic process of EAU, we studied the immune response to IRBP of splenic lymphocytes isolated from blood following IRBP innoculation. Lymphocytes isolated in the early period following innoculation displayed a proliferation response, and anti-IRBP antibo … More dy was identified on the 7th day following innoculation. In addition, antibodies against kidney and RPE were also identified at the same time. Thus we concluded that humoral immunity as well as cellular immunity were important for the development of this type of EAU. (3) Breakdown of the blood-retinal barrier (BRB) is necessary for the development of EAU, however there is a difference between the EAU normally produced by innoculation of Lewis rats and that produced by renal cyrotherapy in BN rats. While damage to the BRB in BN rats depends on antibody development against RPE, in Lewis rats the adhesion molecules ICAM-1 and LFA-1 are important to the breakdown of the BRB. (4) A serum antibody that reacts against ocular tissue, produced in response to renal antigen exposure, was found. (5) However, using an anti-IRBP monoclonal antibody as a ligand for affinity chromatography, we were unable to identify a common renal and ocular tissue antigen. We plan further studies using the antibody described in #4 above. Less