Expression analysis of microRNA during development of murine experimental autoimmune uveoretinitis

小鼠实验性自身免疫性葡萄膜视网膜炎发生过程中microRNA的表达分析

• 批准号: 21791681
• 负责人: HASHIDA Noriyasu
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791681/
• 关键词: 眼免疫学; リポソーム; ドラッグデリバリーシステム; micro RNA; 実験的自己免疫性ぶどう膜炎; 遺伝子発現解析; microRNA

We investigated sequential expression changes in microRNA (miRNA) during the natural course of murine experimental autoimmune uveoretinitis (EAU). Dynamic expression changes of miRNA during EAU were observed from the start of immunization. The expression of most miRNAs changed dramatically from the start of immunization. To search for miRNA that might regulate the inflammatory genes, we examined the potential target genes that might control ocular inflammation in EAU. As a result, up-regulated miR-381 and miR-466b-3-3p expressions, a candidate regulator of the NF-kappaB inhibitor (NFKBIA), down-regulation of miR-322* and miR-465b-5p expression, a regulator of NF-kappaB (NFKBA), occurred on days 5, 15and 20. And also, down-regulation of let-7b* and miR-495, a regulator of IL-10 occurred on days 20 and 30. Dynamic expression changes in miRNAs may be involved in EAU pathogenesis. Our results suggested that changes in the expression levels of specific miRNAs and their candidate target genes are involved in development and remission of EAU.

我们研究了小鼠实验性自身免疫性葡萄膜视网膜炎(EAU)自然病程中microRNA(MiRNA)的连续表达变化。免疫后动态观察小鼠EAU过程中miRNA的表达变化。大多数miRNAs的表达从免疫开始时就发生了巨大的变化。为了寻找可能调节炎症基因的miRNA，我们研究了可能控制EAU眼部炎症的潜在靶基因。结果显示，在第5、15和20天，核因子-kappaB抑制剂(NFKBIA)的候选调节因子miR-381和miR-466b-3-3p的表达上调，而核因子-kappaB(NFKBA)的调节因子miR-322*和miR-465b-5p的表达下调，而IL-10的调节因子let-7b*和miR-495在20和30天表达下调。MiRNAs的动态表达变化可能参与了EAU的发病过程。我们的结果提示，特定miRNAs及其候选靶基因表达水平的变化参与了EAU的发生和缓解。

项目成果

Anterior segment optical coherence tomography findings of presumed intraocular tuberculosis

• DOI: 10.3109/15569527.2010.517231
• 发表时间: 2011-03-01
• 期刊: CUTANEOUS AND OCULAR TOXICOLOGY
• 影响因子: 1.6
• 作者: Hashida, Noriyasu;Terubayashi, Aya;Ohguro, Nobuyuki
• 通讯作者: Ohguro, Nobuyuki

Comprehensive gene-expression profile in murine oxygen-induced retinopathy

• DOI: 10.1136/bjo.2008.142646
• 发表时间: 2008-10
• 期刊: British Journal of Ophthalmology
• 影响因子: 4.1
• 作者: Tatsuhiko Sato;S. Kusaka;N. Hashida;Y. Saishin;T. Fujikado;Y. Tano

日本眼炎症学会学術奨励賞受賞講演炎症部位集積性リポソームを用いたドラッグデリバリーシステムの開発

日本眼科炎症学会学术鼓励奖讲座利用在炎症部位积聚的脂质体开发药物输送系统

• 发表时间: 2009
• 作者: Hashida N;Ohguro N;Houki S;Nishida K.;橋田徳康;橋田徳康
• 通讯作者: 橋田徳康

眼原発悪性リンパ腫に対するリツキシマブ硝子体内投与の効果

玻璃体内注射利妥昔单抗治疗原发性眼部恶性淋巴瘤的效果

• 发表时间: 2009
• 作者: 橋田徳康;西信良嗣;大黒伸行;田野保雄
• 通讯作者: 田野保雄

Expression Analysis of Cytokine and Chemokine Genes during the Natural Course of Murine Experimental Autoimmune Uveoretinitis.

• DOI: 10.5402/2012/471617
• 发表时间: 2012
• 期刊: ISRN inflammation
• 作者: Hashida N;Ohguro N;Nishida K
• 通讯作者: Nishida K