The role of apoE from human monocyte in atherosclerosis

人单核细胞apoE在动脉粥样硬化中的作用

• 批准号: 03671114
• 负责人: TAKAGI Yasushi
• 金额: $ 1.22万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03671114/
• 关键词: monocyte; macrophage; apoE; atherosclerosis; form cell; 貯蔵コレステロール; アポEアイソフォーム; マクロファ-ジ; 貯蔵コレステロ-ル

Monocyte/macrophage plays an important role in developing atherosclerosis. Monocyte would growing form cell, increasing the intima of the vessel, that was atherosclerosis. MOnocyte/macrophage. Secreting apoE was considered the negative factor of developing atherosclerosis This project has a purpose of evaluation the relationship between the dose of secreting apoE and developing atherosclerosis.Human monocyte purified from peripheral blood by centtifugal and plastic adhesion technique was cultured with mediators, such as acetyl LDL and autologous platelet, for 5 days under 95% of O_2 and 5% of CO_2. ApoE in the supernatant of cultured media was measured by immunological method using specific anti-apoE antibody. Cholesterol and cholesterol ester in cultured monocyte/macrophage was measured by enzymatic method. In healthy volunteers.secreting apoE and intaking cholesterol in monocyte/macrophage had big differences between individuals. These results suspect that secreting apoE and stored cholesterol might be aspecific and sensitive markersfor suspecting the development of atherosclerosis in the future. The long term follow-up study should be needed to confirm the significance of secreting apoE measurement.We started to study the measuring significance of secreting apoE in clinical status. At first, we evaluate in diavetes mellitus (DM), because DM was very popular disease and we could get much blood from patient's peripheral vessel. We had many troubles in culturing monocyte/macrophage because of contaminating fungus and bacteria. We could not understand what factor affect these troubles. No laboratory markers, such as blood sugar, HbAlc, C-peptide, and/or urinary sugar did not have thedirect relationship tp the trouble, contamination. We kept going the study in DM.If we could, we would like to evaluate the secreting apoE significance in acute myocardial infarction and cerebral infarction, those were typocal clinical of atherosclerosis.

单核细胞/巨噬细胞在动脉粥样硬化的发生中起重要作用。单核细胞会形成细胞，增加血管内膜，这就是动脉粥样硬化。单核细胞/巨噬细胞。apoE的分泌被认为是动脉粥样硬化发生的不利因素，本项目旨在评价apoE的分泌剂量与动脉粥样硬化发生的关系。采用离心和塑料黏附技术纯化的人外周血单核细胞，以乙酰低密度脂蛋白和自体血小板为培养基，在95%的O_2和5%的CO_2条件下培养5天。用特异性抗ApoE抗体免疫法测定培养基上清中的ApoE。酶法测定培养单核细胞/巨噬细胞中胆固醇和胆固醇酯的含量。在健康的志愿者身上单核细胞/巨噬细胞分泌apoE和摄入胆固醇在个体间存在较大差异。这些结果推测，分泌apoE和储存胆固醇可能是怀疑未来动脉粥样硬化发展的特异性和敏感的标志物。需要长期随访研究来证实apoE分泌测定的意义。我们开始研究apoE在临床状态下的测量意义。首先，我们对糖尿病（DM）进行评估，因为DM是一种非常常见的疾病，我们可以从患者的外周血管获得大量的血液。由于真菌和细菌的污染，我们在单核细胞/巨噬细胞的培养中遇到了很多麻烦。我们不明白是什么因素造成了这些麻烦。没有实验室标记物，如血糖、HbAlc、c肽和/或尿糖与麻烦、污染没有直接关系。如果可以的话，我们想评价apoE在急性心肌梗死和脑梗死中的意义，这是临床上典型的动脉粥样硬化。

项目成果

Miya, T., Takagi, Y., and Gomi, K.: "Platelet and atherosclerosis." Clinical News. 12. 33-38 (1991)

Miya, T.、Takagi, Y. 和 Gomi, K.：“血小板和动脉粥样硬化。”

宮,哲正・高木,康・五味,邦英: "血小板と動脈硬化" クリニカルニュース. 12. 33-38 (1991)

Miya、Tetsumasa、Takagi、Yasushi、Gomi、Kunihide：“血小板和动脉硬化”临床新闻。12. 33-38 (1991)。