The ratio of beta catenin to APC in tumor cell

在癌症预后的诊断中，除了对治疗的反应外，对肿瘤的侵袭和转移潜能的评估是实验室医学领域的重要课题。为了评估对放射治疗或化疗的反应性，我们检测了细胞凋亡通路的完整性。为此，首先分析P53突变或BC12的表达，然后分析细胞凋亡途径中各因子的表达。对于评估肿瘤侵袭和转移潜能的有效方法，分析钙粘素-β连环蛋白的表达被认为是一个可能的候选方法。β-连环蛋白是一种多功能蛋白质，既是粘着连接的组成部分，又是信号转导途径的关键成员。β-连环蛋白的表达水平受泛素-蛋白酶体蛋白降解途径的调节。在该系统中，肿瘤抑制基因APC产物参与了β-连环蛋白的降解，在最近的日本癌症协会年会上，对β-连环蛋白表达与癌细胞状态的关系进行了讨论。在这项研究中，我们试图利用结肠癌标本来检测β-连环蛋白和APC的表达，但没有全面的结果。因此，我们总结分析β-连环蛋白在检验医学癌症诊断中的意义如下。1)癌细胞中β-连环蛋白基因突变的检测。2)分析β-连环蛋白的表达水平取决于癌细胞的来源。3)分析包括APC在内的调节β-连环蛋白表达的分子。

In the diagnosis of prognosis of cancer, the assessment of the invasive and metastatic potential in addition to reactivity to the therapy is significant subject in the field of laboratory medicine. To assess the reactivity to the treatment of either radiation therapy or chemotherapy, the integrity of apoptosis pathway is examined. In this purpose, mutation of p53 or the expression of Bc12 is analyzed firstly and then expression of each factor in the apoptosis pathway is analyzed. The obtained data are profitabley utilized for the clinical practice.For the effective method to assess the invasive and metastatic potential, analysis of the expression of cadherin-beta catenin is considered as a possible candidate. Beta catenin is a multifunctional protein that is both an integral component of adherens junctions and a pivotal member of a signal transduction pathway. The expression level of beta catenin is regulated by ubiquitin-proteasome protein degradation pathway. In the system, tumor suppressor gene APC product contributes the beta catenin degradation.In recent annual meeting of Japanese Cancer Association, the relation of beta catenin-expression and status of the cancer cells have been discussed. In this study, we attempted to examine the expression of beta catenin and APC using specimens from colon cancer, but did not comprehensive results. Therefore, we summarize the significance of analysis of beta catenin for cancer-diagnosis in laboratory medicine as follows. 1)Detection of the mutation of beta catenin gene in cancer cell. 2)Analysis of the expression level of beta catenin depends on the origin of cancer cell. 3)Analysis of the molecules including APC that regulate the expression of beta catenin.