Serum CK isoenzyme - Gene analysis of hyperCK-BB patient -

血清CK同工酶-hyperCK-BB患者基因分析-

• 批准号: 11672308
• 负责人: TAKAGI Yasushi
• 金额: $ 1.92万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672308/
• 关键词: creatine kinase; CK isoenzyme; high CK-BB activity; gene analysis; CK-B活性; 心筋梗塞

Creatine kinase (CK) plays an important role in energy supply in muscle contraction. CK has three isoenzymes, CK-MM, CK-MB, and CK-BB in cytoplasm, and one isoenzyme, mCK in mitochondria. CK-MB is analyzed in clinical laboratory as an indicator for cardiac injury, and CK-BB is analyzed as an indicator for brain damage or adenocarcinoma. Abnormal high level of CK-BB, 37%, was found in 15-year boy, and his elder sister had similar high level of CK-BB, 47%. They had no cardiac injury or brain damage and carcinoma. Gene analysis of their blood was performed and no abnormal gene was detected. We performed screening tests for detecting an abnormal high level of CK.BB and/or CK.B activity. CK-B activity was measured by immunoinhibition method. Molecular weight of CK was measured by thin-layer gel-filtration method and activation energy was measured for distinguished abnormal CK from CK-linked immunoglobulin or mCK. We measured CK-B activity in 3,015 patients serum. Two-hundred eighty seven patients had abnormal high level of CKB/T-CK, above 0.1. According to their chief complaints and other clinical tests reveled 231 patients of cardiac injury, 15 of thyroid diseases, 15 of brain damage, 9 of malignant tumor, 9 of infant, and 12 patients had no other diseases or damage. We could not find out any abnormal CK-B needed for gene analysis.

肌酸激酶（CK）在肌肉收缩过程中起着重要的能量供应作用。CK在细胞质中有三种同工酶CK- mm、CK- mb和CK- bb，在线粒体中有一种同工酶mCK。临床实验室分析CK-MB作为心脏损伤的指标，CK-BB作为脑损伤或腺癌的指标。15岁男孩CK-BB异常高，为37%，其姐姐CK-BB异常高，为47%。他们没有心脏损伤、脑损伤和癌症。对他们的血液进行基因分析，未发现异常基因。我们进行了筛选试验，以检测异常高水平的CK。BB和/或CKB的活动。免疫抑制法测定CK-B活性。用薄层凝胶过滤法测定CK的分子量，并测定活化能，以区分异常CK与CK连锁免疫球蛋白或mCK。我们测量了3015例患者血清中CK-B的活性。287例患者CKB/T-CK异常高，高于0.1。根据主诉及其他临床检查结果，心脏损伤231例，甲状腺疾病15例，脑损伤15例，恶性肿瘤9例，婴儿9例，无其他疾病或损伤12例。我们没有发现任何需要基因分析的异常CK-B。

项目成果

Blood tests for acute myocardial infarction.

急性心肌梗死的血液检查。

• 发表时间: 2002
• 期刊: Emergency Nurcing 15-1
• 作者: Yasushi Takagi

Correspondence to high CK activity in serum.

对应于血清中的高CK活性。

• 发表时间: 2001
• 期刊: Nippon Iji-shinpo 4037
• 作者: Kazumi Taguchi;Yasushi Takagi
• 通讯作者: Yasushi Takagi

高木 康: "クレアチンキナーゼ(CK)"臨床病理レビュー(特集). 116号. 52-61 (2001)

Yasushi Takagi：“肌酸激酶 (CK)”临床病理学评论（特刊）第 116. 52-61 (2001)。

血中ミオグロビンとトロポニンTの迅速測定-カーディアックリーたーによる定量-

快速测量血液肌红蛋白和肌钙蛋白 T - 通过 Cardiac Retar 定量 -

• 发表时间: 2001
• 期刊: 臨床検査機器試薬 24・4
• 作者: 田口和三;五味常明;高木康;五味邦英
• 通讯作者: 五味邦英

Rapid measurement of cardiac markers by immunochromatography-Myoglobin and troponin-T by cardiac reader

免疫层析法快速测量心脏标志物——心脏阅读器上的肌红蛋白和肌钙蛋白-T

• 发表时间: 2001
• 期刊: Journal of Analytical Bio-Science 24-4
• 作者: 田口和三;五味常明;高木康;五味邦英;Yasushi Takagi
• 通讯作者: Yasushi Takagi