Biochemical Study on Muscle Glycogen Supercompensation by Carbohydrate Feeding

碳水化合物喂养肌糖原超补偿的生化研究

• 批准号: 03680131
• 负责人: HIGUCHI Mitsuru
• 金额: $ 0.32万
• 依托单位: National Institute of Health and Nutrition
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680131/
• 关键词: muscle; exercise; GLUT4; glucose; glycogen; 糖輸送体; 一過性運動; 筋活動状態; グリコ-ゲン

In the present study, we examined whether glucose transporter in rat skeletal muscle (GLUT4) plays an important role in the process of muscle glycogen resynthesis after endurance exercise followed by glucose ingestion. Glycogen concentration in soleus muscle decreased by 25% in response to 120-min treadmill running. and increased by 100% 3 hours after glucose ingestion immediately after exercise. Muscle glycogen decreased to the level of pre-exercise state 24 hours after exercise. No significant changes were observed in GLUT4 concentration in soleus muscle during recovery period of 120-min exercise. An exhaustive running (@5.5 hours) induced significant decrease in glycogen concentration of soleus muscle, but 2-fold of muscle glycogen concentration was observed 24 hours after the exercise. GLUT4 in soleus decreased by 30% immediately after the exhaustive running, but recovered to the pre-exercise level. These results suggest that GLUT4 does not play an important role to modulate an increase of glucose uptake after endurance exercise followed by glucose ingestion.

在本研究中，我们研究了大鼠骨骼肌葡萄糖转运蛋白(GLUT4)在耐力运动后摄取葡萄糖后肌糖原再合成过程中是否起重要作用。在跑步机上跑120分钟后，比目鱼肌糖原浓度降低了25%。运动后即刻摄取葡萄糖后3h增加100%。运动后24小时肌糖原降至运动前水平。120min运动后恢复期比目鱼肌GLUT4浓度无明显变化。力竭跑(5.5h)导致比目鱼肌糖原浓度显著下降，但运动后24h肌糖原浓度增加了2倍。比目鱼肌GLUT4在力竭跑后即刻下降30%，但恢复到运动前水平。这些结果表明，GLUT4在调节耐力运动后葡萄糖摄取增加的过程中不起重要作用。

项目成果

EZAKI,O: "Exercise training increases glucose trnsporter content in skeletal muscle more efficiently from obese rats than young lean rats." Diabetes. 41. 920-926 (1992)

EZAKI,O：“与年轻的瘦大鼠相比，运动训练可以更有效地增加肥胖大鼠骨骼肌中葡萄糖转运蛋白的含量。”