Biochemical characterization of endothelin converting enzyme

内皮素转化酶的生化表征

• 批准号: 03680136
• 负责人: KIMURA Sadao
• 金额: $ 1.41万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680136/
• 关键词: endothelin; big endothelin; endothelin converting enzyme; phosphoramidon; ACHN細胞

1. Two phosphoramidon sensitive-neutral metalproteases (M1 and M2) with endothelin converting activity were detected in membranous fractions of porcine lung. M1 specifically converts only big ET-1 to ET-1 not big ET-2 and big ET-3 to ET-2 and ET-3, respectively. Taking together with other biochemical analyses, it is suggested that M1 is a new physiological enzyme and M2 may be a known neutral endopeptidase2. (i) Analysis of endothelin-2 (ET-2) biosynthetic pathway showed that ET-2 is produced via big ET-2(1-38) as an intermediate form. The biochemical characterization of ET-2 converting enzyme (ECE-2) indicates that ECE-2 is a phosphoramidon-sensitive metal protease and very similar to endothelin-1 converting enzyme (ECE-1) from endothelial cells.

1. 在猪肺膜组织中检测到两种具有内皮素转化活性的磷酰胺敏感中性金属蛋白酶（M1和M2）。M1特异性地只将大ET-1转化为ET-1，而不是大ET-2和大ET-3分别转化为ET-2和ET-3。结合其他生化分析，M1可能是一种新的生理酶，M2可能是一种已知的中性内多肽酶e2。(i)对内皮素-2（ET-2）生物合成途径的分析表明，ET-2是通过大ET-2（1-38）作为中间形式产生的。ET-2转化酶（ECE-2）的生化特性表明，ECE-2是一种磷酰胺敏感的金属蛋白酶，与内皮素-1转化酶（ECE-1）非常相似。

项目成果

木村 定雄: "エンドセリンの検査法について" 医学検査. 41. 813-817 (1992)

Sadao Kimura：“关于内皮素的检测方法”医学检测。41. 813-817（1992）。

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SAWAMURA,T.et al.：“猪滤膜中磷酰胺敏感性金属蛋白酶与内皮素转化酶活性的表征”Biochim.Biophys.Acta。

Sakurai, T., Yanagisawa, M., Inoue, A., Ryan, U., Kimura, S., Mitsui, Y., Goto, K., Masaki, T.: "cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA." Biochem. Biophys. Res. Commun.175. 44-47 (1991)

Sakurai, T.、Yanagisawa, M.、Inoue, A.、Ryan, U.、Kimura, S.、Mitsui, Y.、Goto, K.、Masaki, T.：“cDNA 克隆、序列分析和组织分布

木村 定雄: "エンドセリンファミリーペプチドの構造と機能及びプロセシング酵素による活性化機構" Cell Science. 8. 317-325 (1992)

Sadao Kimura：“内皮素家族肽的结构和功能以及加工酶的激活机制”《细胞科学》8. 317-325 (1992)。

Giaid,A,et al.: "Expression of endo thelin-like peptide in the nervous system of the marine mollusk Aplysia" J.Cardiovasc.Pharmacol. 17(Suppl.7). S449-S451 (1991)

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