P05 –Comparative exploration of KEA3 function in PMF partitioning and photoprotection

P05 âKEA3在PMF分区和光保护中功能的比较探索

基本信息

项目摘要

The chloroplast PMF is produced via light-driven proton-coupled electron transport across the thylakoid membrane. This generates both a membrane potential (∆ψ) and a proton concentration gradient (∆pH). Their contribution to PMF is governed in part by thylakoid ion transport processes. The ∆pH component of the PMF or more specifically, the lumenal proton concentration determines the fraction of light energy that is used for photochemistry. Our data from Arabidopsis thaliana (Arabidopsis) show that the thylakoid K+-exchange antiporter 3 (KEA3) plays a fundamental role for the flexibility of vascular plant photosynthesis during light fluctuations. KEA3 can sense the energy state of the chloroplast stroma and adjusts levels of the photoprotective energy dependent quenching (qE) mechanism in response. If the energy state is low, KEA3 gets activated to decrease the proton concentration of the lumen and shuts off qE. While KEA3 activity affects PMF composition and decreases the ∆pH component, it appears to have little effect on PMF size and thus its energetic function. Previously, we could demonstrate that de-regulated KEA3 activity can result in transiently higher CO2 fixation rates. This proposes KEA3 regulation as a putative target for enhancing photosynthesis under the dynamic light conditions found in nature. An in-depth understanding of KEA3 regulation and its interaction with the complex network of photosynthetic reactions will be pivotal to further explore this avenue. Within the framework of GoPMF, we seek to extent our knowledge of KEA3 function in PMF partitioning and its regulation via a comparative approach: (i) By measuring KEA3 activity in the unicellular alga Chlamydomonas reinhardtii (Chlamydomonas), we aim to understand evolutionary effects on KEA3 function and regulation, (ii) by comparing stromal energy dynamics between Arabidopsis and Chlamydomonas, we aim to characterize how environmental changes affect the chloroplast energy state and relate this to KEA3 activity , (iii) by determining KEA3 regulating factors, we will link changes in the stromal energy state with KEA3 activity and finally (iv) by expressing modified KEA3 versions in planta, we will confirm these links experimentally and use our knowledge on PMF modulation by KEA3 for attempts to improve photosynthetic performance and growth (see also Graphical Abstract P05). Our work will be integral for the aim GoPMF 2 and additionally contribute to aims 1, 3, 4 and 5.
叶绿体PMF是通过光驱动的质子耦合电子传递穿过类囊体膜而产生的。这会产生膜电位(∆ψ)和质子浓度梯度(∆pH)。它们对PMF的贡献在一定程度上是由类囊体离子传输过程决定的。PMF的∆pH分量,或者更具体地说,管腔质子浓度决定了用于光化学的光能的比例。我们来自拟南芥的数据表明,类囊体钾交换逆向转运蛋白3(KEA3)对维管植物光合作用在光波动中的灵活性起着基础性的作用。KEA3可以感知叶绿体基质的能量状态,并相应地调节光保护能量依赖猝灭(QE)机制的水平。如果能量状态较低,KEA3会被激活,以降低管腔中的质子浓度,并关闭QE。虽然KEA3活性影响了PMF的组成,降低了∆的pH分量,但对PMF的大小和能量功能的影响似乎很小。以前,我们可以证明,去调节KEA3活性可以导致短暂的更高的二氧化碳固定率。这提出了KEA3调节作为在自然界中发现的动态光条件下增强光合作用的假定目标。深入了解KEA3的调控及其与复杂的光合作用反应网络的相互作用将是进一步探索这一途径的关键。在GoPMF的框架内,我们试图通过比较的方法来扩展我们对KEA3在PMF分配和调控中的功能的了解:(I)通过测量单细胞藻类Chlamydomonas rehardtii(Chlamydomonas)中的KEA3活性,我们旨在了解KEA3功能和调控的进化效应;(Ii)通过比较拟南芥和衣藻的基质能量动态,我们旨在表征环境变化如何影响叶绿体能量状态,并将其与KEA3活性联系起来;(Iii)通过确定KEA3调节因子,我们将基质能量状态的变化与KEA3活性联系起来,最后,通过在Planta、Planta、Chlamydomonas中表达KEA3修饰版本,我们将把基质能量状态的变化与KEA3活性联系起来我们将通过实验证实这些联系,并利用我们对KEA3调制PMF的知识来尝试改善光合作用表现和生长(另见图形摘要P05)。我们的工作将是GoPMF2目标不可或缺的一部分,并对目标1、3、4和5作出额外贡献。

项目成果

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Professorin Dr. Ute Armbruster其他文献

Professorin Dr. Ute Armbruster的其他文献

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{{ truncateString('Professorin Dr. Ute Armbruster', 18)}}的其他基金

Flux4LIVES_Thylakoid ion flux-Linking photosynthetic efficiency with osmotic stress response
Flux4LIVES_类囊体离子通量-将光合效率与渗透胁迫响应联系起来
  • 批准号:
    355293141
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Regulation of photosynthesis by thylakoid K+/H+ antiport
类囊体 K/H 反向端口对光合作用的调节
  • 批准号:
    366065941
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Characterization of the genetic requirements for photoautotrophic growth under fluctuating light intensities, using novel high-throughput genomics approaches in the green alga Chlamydomonas reinhardtii
使用绿藻莱茵衣藻的新型高通量基因组学方法表征波动光强下光自养生长的遗传要求
  • 批准号:
    211381283
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Research Fellowships

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    2018
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SHF: Medium: Collaborative Research: Toward Extreme Scale Fault-Tolerance: Exploration Methods, Comparative Studies and Decision Processes
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A Comparative Sociological Exploration of Science Curriculum : Focus on a GCSE Science Textbook in England
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