Development of superbioreactors

A.催化装置的发展(1)化学修饰对酶功能的转化：假单胞菌脂肪酶的酪氨酸残基经过化学修饰后，其酯酶活性急剧下降，而脂肪酶活性几乎没有受到影响。当该酶的两个酪氨酸残基被修饰时，观察到了这种现象。(2)寻找有效转化非天然化合物的生物催化剂：尝试了2-三甲基硅烷(TMS)-1-丙醇(A)、1-TMS-2-丙醇(B)和1-TMS-1-丙醇(C)的酶促光学拆分。与化学方法不同的是，对于A和B与5-苯基戊酸的酯化反应，酶促方法具有更好的效果。此外，假单胞菌对B的光学分辨率也达到了很高的水平。脂蛋白脂酶。无载体生物催化剂的发展在饱和四氯化碳-异辛醇(8：2)中，催化dl-2-(4-氯苯氧基)丙酸和肉豆蔻醇(8：2)的立体选择性酯化反应在30ﾟC下，酵母脂肪酶360催化，酶粉的反应速度和立体选择性是硅藻土吸附酶的3倍。作为应用，开发了一种连续反应器，在其中填充了酶粉的玻璃柱。该体系的产酶效率是硅藻土吸附酶的4倍。

A.Development of catalytic devices (1)Conversion of enzyme functions by chemical modification: Upon chemical modification of tyrosine residues of a Pseudomonaslipase, its esterase activity was reduced drastically, while lipase activity was little affected. This phenomenon was observed when two of the tyrosine residues of this enzyme were modified. (2) Search of biocatalysts effective for transformation of unnatural compounds: Enzymatic optical resolution of 2-trimethylsily(TMS)-1- propanol(A),1-TMS-2-propanol(B) and 1-TMS-1-propanol(C) was attempted. Unlike chemical methods, enzymatic methods were found to be superior for esterifying A and B with 5-phenylvaleric acid. Furthermore, efficient optical resolution was achieved for B with Pseudomonas sp. lipoprotein lipase. Because of a steric bulk, C reacted more slowly than A and B. but a significant stereoselectivity was observed for C as well.B.Development of support-free biocatalystStereoselective esterification of dl-2-(4-chlorophenoxy) propionic acid with myristyl alcohol was carried out in water-saturated carbon tetrachloride-issoctanol (8:2) in the presence of yeast lipase OF360 at 30ﾟC. A 3-fold higher rate and high stereoselectivity was observed for enzyme powder than that for celite-adsorbed enzyme. As an application a continuous reactor was developed in which a glass column was filled with enzyme powder. This system yielded a 4-fold higher productivity than the celite-adsorbed enzyme.