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THE DEVELOPMENT AND THE CLINICAL APPLICATION OF AN OPTICAL SYSTEM FOR THE SIMULTANEOUS DETERMINATION OF METABOLIC AND FUNCTIONAL CHANGES IN THE HEART AND BLOOD VESSELS.

用于同时测定心脏和血管代谢和功能变化的光学系统的开发和临床应用。

基本信息

批准号：
03557043
负责人：
KANAIDE Hideo
金额：
$ 7.17万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

(1) The development of a system for the simultaneous determination of metabolic and functional changes in the heart and blood vessels : A front-surface fluorometer with concentric optical fibers for double-wave length excitation (inner circle quartz fibers) and fluorescence detection (outer circle glass fibers) (CAM OF-2) was successfully and specifically designed and made with the collaboration of Japan Spectroscopic Co., Tokyo, Japan.(2) Using front-surface fluorometry of fura-2-loaded aortic valvular strips, the cytosolic Ca concentration, (Ca)i, of the endothelial cells in situ was firstly and quantitatively recorded. Both endothelin (ET)-1 and ET-3 elevated (Ca)i in the endothelial cells in situ. ET-1 elevated (Ca)i of a peak (the first phase) and sustained (the second phase) type. The second phase was exclusively extracellular Ca-dependent, Ca influx. Pertussis toxin (IAP) markedly inhibited the second phase. Thus, Ca influx induced by ET-1 is regulated by an IAP-sensitive G-protein in the endothelial cells in situ.(3) The effects of ethanol on the contractility of strips of porcine coronary artery, with and without endothelium, and following permeabilization with alpha -toxin, and on aortic valvular endothelial cells in situ were examined. It was found that ethanol contracted the coronaty artery both by increasing (Ca)i and by raising Ca sensitivity of the contractile apparatus, as mediated by GTP-binding protein. When the endothelium was exposed to ethanol, this contraction was prevented, presumably by releasing endothelium-derived relaxing factor (EDRF).(4) Using front-surface fluorometry and BCECF-loaded isolated perfused rat heart, pH changes of the myocardium was successfully recorded during ischemia-reperfusion. It was found that captoril, an angiotensin converting enzyme inhibitor, could partially inhibit the development of acidosis during ischemia.

(1)同时测定心脏和血管中代谢和功能变化的系统的开发：与Japan Spectroscopic Co.合作，成功地专门设计并制造了具有用于双波长激发（内环石英纤维）和荧光检测（外环玻璃纤维）的同心光纤的前表面荧光计（CAM OF-2），日本东京(2)利用荧光法测定了Fura-2负载的主动脉瓣条的前表面，首次定量记录了原位内皮细胞的胞浆Ca浓度（Ca）i。内皮素（ET）-1和ET-3均使内皮细胞内Ca ~（2+）升高。ET-1升高（Ca）i呈高峰（第一时相）和持续（第二时相）型。第二阶段是完全细胞外钙依赖性，钙内流。百日咳毒素（IAP）明显抑制第二时相。因此，由ET-1诱导的Ca内流在内皮细胞中由IAP敏感的G蛋白原位调节。(3)本文研究了乙醇对猪冠状动脉条（有内皮和无内皮）和α-毒素透化后收缩性的影响，以及对原位主动脉瓣内皮细胞的影响。结果表明，乙醇收缩冠状动脉的作用是通过增加（Ca）i和提高收缩器官的Ca敏感性两种途径实现的，这是由GTP结合蛋白介导的。当内皮细胞暴露于乙醇时，这种收缩被阻止，可能是通过释放内皮源性舒张因子（EDRF）。(4)应用前表面荧光法和负载BCECF的离体灌流大鼠心脏，成功地记录了缺血-再灌注过程中心肌pH的变化。发现血管紧张素转换酶抑制剂captoril可部分抑制缺血时酸中毒的发展。