Non-invasive blood glucose measurement using infrared absorption spectroscopy with a CO_2 laser - design for miniaturization

使用 CO_2 激光红外吸收光谱进行无创血糖测量 - 小型化设计

• 批准号: 03557119
• 负责人: SHICHIRI Motoaki
• 金额: $ 11.14万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557119/
• 关键词: NON-INVASIVE MEASUREMENTS OF BLOOD GLUCOSE CONCENTRATION; FOURIER TRANSFORM INFRARED ABSORBANCE SPECTROSCOPY; ATENUATED TOTAL REFLECTION PRISM; DIFFERENCE ABSORBANCE; ORAL MUCOSA; PRISM ATTACHMENT; CARBON DIOXIDE LASER; フーリエ変換赤外分光; 減衰全反射装置; 非侵襲的血

The non-invasive glucose measurement by analyzing Fourier transform infrared (FTIR) absorbance spectra through oral mucosa with an attenuated total reflection (ATR) prism was developed as an alternative way of long-term glycemic monitoring. In all experiments, the output from the ATR prism (zinc selenide) was detected by a MCT or TGS detector. The technique was based on the phenomena that the absorption intensity at a glucose-specific peak was directly related to the glucose concentration within a very thin layer of the sample adjacent to an ATR prism. In glucose aqueous solutions, glucose had a characteristic absorption at 1033 cm^<-1> and the absorption intensity was proportional to the glucose concentration. In serum and whole blood samples, however, red blood cell corpuscles and serum proteins interfered with the absorbance spectra of glucose and shifted the base line upward significantly. Therefore, to eliminate these interferences, the feasibility of the calibration curves obtain … More ed by using difference absorbance spectra with those of fasting samples was studied. As a result, highly significant correlation between the increases in the absorption intensities above those of fasting samples and the increases in glucose concentrations above those of fasting samples. From these experiments, it was concluded that by infrared spectroscopy glucose concentrations in serum and whole blood samples could be measured quantitatively and monitored. In absorbance spectra through oral mucosa, after correcting the spectral changes due to the prism attachment by using absorption peaks at 2920 cm^<-1>(CH_2 band) as reference peaks, high correlation between the second derivatives of absorbance spectra and blood glucose concentrations was observed by the use of a similar approach. In conclusion, it was demonstrated that Fourier transform infrared spectroscopy could be useful for non-invasive monitoring of blood glucose through oral mucosa. A carbon dioxide laser, furthermore, was introduced as a light source in Fourier transform infrared spectrometer for miniaturization and improvement of the sensitivity. Not only the achievement in stable output of laser but also the development of a protection system against laser beam and a laser beam divergence system enabled us to establish the non-invasive measurement of blood glucose concentrations based on spectral analysis. Less

利用衰减全反射棱镜(ATR)分析口腔粘膜傅里叶变换红外(FTIR)吸收光谱的无创血糖测量方法是一种可供选择的长期血糖监测方法。在所有实验中，ATR棱镜(硒化锌)的输出由MCT或TGS探测器检测。这项技术是基于这样的现象，即葡萄糖专有峰处的吸收强度与样品中靠近ATR棱镜的非常薄的一层内的葡萄糖浓度直接相关。在葡萄糖水溶液中，葡萄糖在1033 cm~(-1)~(-1)处有一个特征吸收峰，吸收强度与葡萄糖浓度成正比。然而，在血清和全血样本中，红细胞和血清蛋白干扰了葡萄糖的吸收光谱，使基线显著上移。因此，为了消除这些干扰，对标定曲线进行可行性分析，得到…并进一步研究了与禁食样品的差示吸收光谱。结果，高于禁食样本的吸收强度的增加与高于禁食样本的葡萄糖浓度的增加之间存在高度显著的相关性。实验结果表明，红外光谱分析技术可以对血清和全血中的葡萄糖浓度进行定量测量和监测。在口腔粘膜吸收光谱中，用2920 cm~(-1)&gt；(CH_2)吸收峰作为参照峰，校正由于附着棱镜引起的光谱变化后，用类似的方法观察到吸收光谱的二阶导数与血糖浓度之间的高度相关性。综上所述，傅立叶变换红外光谱分析技术可用于口腔黏膜血糖的无创监测。此外，在傅里叶变换红外光谱仪中引入了二氧化碳激光器作为光源，以实现红外光谱仪的小型化和提高灵敏度。不仅激光输出稳定，而且激光防护系统和激光发散系统的研制使我们能够建立基于光谱分析的血糖浓度无创测量。较少

项目成果

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Kajiwara K.et al.: "Non-invasive Measurement of Blood Glucose Concentrations by Analysing Fourier Transform Infrared Absorbance Spectra through Oral Mucosa." Medical & Biological Engineering & Computing. (1992)

Kajiwara K.等人：“通过口腔粘膜分析傅里叶变换红外吸收光谱来无创测量血糖浓度。”

Kajiwara,K.,Fukushima,H.,Kishikawa,H.,Nishida,K.,Hashiguchi,Y.,Sakakida,M.,Uehara,M.,Shichiri,M.: "Spectroscopic quantitative analysis of blood glucose by Fourier transform infrared spectroscopy with an attenuated total reflection prism." Medical Progress

Kajiwara，K.，Fukushima，H.，Kishikawa，H.，Nishida，K.，Hashiguchi，Y.，Sakakida，M.，Uehara，M.，Sichiri，M.：“通过傅里叶变换对血糖进行光谱定量分析

Kajiwara K., Fukushima H., Sakakida M. and Shichiri M.: "Development of artificial endocrine pancreas-Possibility of non-invasive measurement of blood glucose using infra-red spectroscopy with an ATR prism-." Recent advances in Insulin Therapy, Edited by

Kajiwara K.、Fukushima H.、Sakakida M. 和 Shichiri M.：“人工内分泌胰腺的开发 - 使用 ATR 棱镜红外光谱仪无创测量血糖的可能性 -”。

Fukushima H.,Kajiwara K.,Sakakida M. and Shichiri M.: "Noninvasive blood glucose measurement system as a glucose monitoring device in an artificial endocrine pancreas." Artificial Organs 14. Suppl 3. 38-42 (1990)

Fukushima H.、Kajiwara K.、Sakakida M. 和 Shichiri M.：“无创血糖测量系统作为人工内分泌胰腺中的血糖监测装置。”