Establishment of Analytical System for Metabolite Transport in Animal and Plant Organelles

动植物细胞器代谢物转运分析体系的建立

• 批准号: 03559002
• 负责人: YAMAGUCHI Junji
• 金额: $ 8.06万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03559002/
• 关键词: Organelle; Hormone; Nuclei; Amyloplast; Chloroplast; Centrifugation; アミロプラスト; 物質透過; 輸送機構; シリコンオイル; ホモジナイザ-; 単離

(1) Separation and fractionation of intact organelles from animal and plant cells and(2) establishment of precise and rapid analytical system for metabolite transport are final aiming in the project. As a first step, we attempted to make improved tissue homogenizer for aim (1) and to make new centrifugation system for aim (2). Using these systems, we succeeded in isolating nuclei, amyloplasts and chloroplasts from plant cells and confirmed the hypothesis that ADPglucose is directly transported into amyloplasts.

(1)动植物细胞完整细胞器的分离和分级；(2)建立准确、快速的代谢物转运分析系统是本项目的最终目标。作为第一步，我们试图为AIM(1)制作改进的组织匀浆器，并为AIM(2)制作新的离心机。利用这些系统，我们成功地从植物细胞中分离出细胞核、淀粉体和叶绿体，并证实了ADP葡萄糖直接运输到淀粉体的假说。

项目成果

J.Pozueta-Romero: "Direct transport of ADP glucose by an adenylate translocator is linked to starch biosynthesis in amyloplasts" Proc.Natl.Acad.Sci.USA. 88. 5769-5773 (1991)

J.Pozueta-Romero：“腺苷酸转运蛋白直接转运 ADP 葡萄糖与造粉体中的淀粉生物合成有关”Proc.Natl.Acad.Sci.USA。

山口 淳二: "種子貯蔵デンプン分解系." 植物細胞工学. (1993)

Junji Yamaguchi：“种子储存淀粉分解系统。”（1993）

J. Pozueta-Romero: "Comparative analysis of mitochondrial and amyloplast adenylate translocators" FEBS Lett.287. 62-66 (1991)

J. Pozueta-Romero：“线粒体和造粉体腺苷酸易位子的比较分析”FEBS Lett.287。

J. Yamaguchi: "Isolation and characterization of nuclei form rice embryos" Cell Struc. Func.(1992)

J. Yamaguchi：“水稻胚胎细胞核的分离和表征”Cell Struc。

J.Pozueta-Romero: "ADPG formation by the ADP-specific cleavage of sucrose-reassessment of sucrose synthase." FEBS Lett.291. 233-237 (1991)

J.Pozueta-Romero：“通过蔗糖的 ADP 特异性裂解形成 ADPG——蔗糖合酶的重新评估。”