Establishment of Analytical System for Metabolite Transport in Animal and Plant Organelles

(1)从动植物细胞中分离和分离完整的细胞器；(2)建立精确、快速的代谢物转运分析系统是本项目的最终目标。作为第一步，我们尝试为目标(1)制造改进的组织均质机，并为目标(2)制造新的离心系统。利用这些系统，我们成功地从植物细胞中分离出细胞核、淀粉体和叶绿体，并证实了ADPglucose直接转运到淀粉体中的假设。

(1) Separation and fractionation of intact organelles from animal and plant cells and(2) establishment of precise and rapid analytical system for metabolite transport are final aiming in the project. As a first step, we attempted to make improved tissue homogenizer for aim (1) and to make new centrifugation system for aim (2). Using these systems, we succeeded in isolating nuclei, amyloplasts and chloroplasts from plant cells and confirmed the hypothesis that ADPglucose is directly transported into amyloplasts.