Establishment of Analytical System for Metabolite Transport in Animal and Plant Organelles

(1)动植物细胞完整细胞器的分离和分级；(2)建立准确、快速的代谢物转运分析系统是本项目的最终目标。作为第一步，我们试图为AIM(1)制作改进的组织匀浆器，并为AIM(2)制作新的离心机。利用这些系统，我们成功地从植物细胞中分离出细胞核、淀粉体和叶绿体，并证实了ADP葡萄糖直接运输到淀粉体的假说。

(1) Separation and fractionation of intact organelles from animal and plant cells and(2) establishment of precise and rapid analytical system for metabolite transport are final aiming in the project. As a first step, we attempted to make improved tissue homogenizer for aim (1) and to make new centrifugation system for aim (2). Using these systems, we succeeded in isolating nuclei, amyloplasts and chloroplasts from plant cells and confirmed the hypothesis that ADPglucose is directly transported into amyloplasts.