Characterization of a mutant with abnormal flagellar morphogenesis in Chlamydomonas reinhardtii.
莱茵衣藻鞭毛形态发生异常突变体的表征。
基本信息
- 批准号:03640581
- 负责人:
- 金额:$ 1.09万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
I have characterized a novel flagellar mutant in Chlamydomonas reinhardtii. When the mutant was cultured under the light (-4,800 lux), most cells had no flagella. However, when the cultures were put in the dark, greater than 70% of the cells flagellated within 16 h after the transfer. The flagellar morphology varied from "rod-shape" (same as the wild-type flagella) to "disk-shape" . Electron microscopy showed that the disk-shaped flagella had the axonemes which were curved into a loop within the swollen membrane. On the other hand, flagellated cells (70%*) in the dark were put under the light, all cells had no flagella within 8 h after the transfer. Light inhibition of flagellation was restored in the presence of 10^<-6>-10^<-5> M DCMU. The spectral dependency of the photo-inhibition of flagellation showed maximal effectiveness at 400-420nmand 660-680nm. Flagellar regeneration of the mutant was also inhibited by light, and the inhibition pattern was similar to that of cycloheximide (20 mug/ml). Axonemes of the mutant maintained looped shape after being demembranated with 0.5% Nonidet P-40. SDS PAGE of the mutant flagella showed that two bands with molecular weights of 250 kDa and 7.4 kDa were missing and three other bands with molecular weights 240 kDa, 150 kDa and 130 kDa increased their densities. From these results, I suppose that product(s) of photosynthesis inhibit flagellation of the mutant, and formed flagella have defect(s) in the binding between the axoneme itself. Tetrad analysis showed that phenotypes of the mutant resulted from a single gene mutation.
我的特点是一个新的鞭毛突变体在莱茵衣藻。当突变体在光照(~ 4,800 lux)下培养时,大多数细胞没有鞭毛。然而,当将培养物置于黑暗中时,超过70%的细胞在转移后16小时内鞭毛化。鞭毛形态从“杆状”(与野生型鞭毛相同)到“盘状”。电子显微镜观察发现,盘状鞭毛内有轴丝,轴丝在膨大的膜内弯曲成环状。另一方面,将黑暗中的鞭毛细胞(70%*)置于光照下,所有细胞在转移后8小时内没有鞭毛。在10^-10 ^M DCMU存在下,鞭毛形成的光抑制恢复<-6><-5>。在400- 420 nm和660- 680 nm波长范围内,光抑制的效果最好。突变体的鞭毛再生也被光抑制,抑制模式与放线菌酮(20 μ g/ml)相似。突变体的轴丝保持环状后,被脱膜与0.5%的Nonidet P-40。突变体鞭毛的SDS-PAGE显示,分子量为250 kDa和7.4 kDa的2条条带缺失,分子量为240 kDa、150 kDa和130 kDa的3条条带密度增加。从这些结果,我认为光合作用的产物抑制了突变体的鞭毛形成,并且形成的鞭毛在轴丝本身之间的结合中存在缺陷。四联体分析表明,突变体的表型是由单基因突变引起的。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakamura, Shogo: "A new mutant with looped axonemes in Chlamydomonas." Cell Struct. Funct.17. 522 (1992)
Nakamura,Shogo:“衣藻中具有环状轴丝的新突变体。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
中村 省吾: "単細胞緑藻クラミドモナスの鞭毛の形態形成に及ぼす光の影響" 東京大学海洋研究所大槌臨海研究センタ-報告.
Shogo Nakamura:“光对单细胞绿藻衣藻鞭毛形态发生的影响”来自东京大学海洋研究所大槌海洋研究中心的报告。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
中村 省吾: "A new mutant with looped axonemes in Chlamydomonas." Cell Struct.Funct.17. 522 (1992)
Shogo Nakamura:“衣藻中具有环状轴丝的新突变体。”Cell Struct.Funct.17(1992)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
中村 省吾: "A new mutant with looped axonemes in chlamydomonas." Cell Struct Funct.17. 522- (1992)
Shogo Nakamura:“衣藻中具有环状轴丝的新突变体。”17-522。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
NAKAMURA Shogo其他文献
Optimization and Hole Interpolation of 2-D Sparse Arrays for Accurate Direction-of-Arrival Estimation
二维稀疏阵列的优化和孔插值以实现准确的波达方向估计
- DOI:
10.1587/transcom.2020ebp3035 - 发表时间:
2021 - 期刊:
- 影响因子:0.7
- 作者:
NAKAMURA Shogo;IWAZAKI Sho;ICHIGE Koichi - 通讯作者:
ICHIGE Koichi
DOA-Based Weighted Spatial Filter Design for Sum and Difference Composite Co-Array
基于 DOA 的和差复合共阵加权空间滤波器设计
- DOI:
10.1587/transcom.2019ebp3213 - 发表时间:
2020 - 期刊:
- 影响因子:0.7
- 作者:
IWAZAKI Sho;NAKAMURA Shogo;ICHIGE Koichi - 通讯作者:
ICHIGE Koichi
MEASUREMENTS FOR EMISSION SPECTRUM OF LIQUID XENON
液态氙发射光谱的测量
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0
- 作者:
FUJII Keiko;TORIGOE Yui;ENDO Yuya;NAKAMURA Shogo;TAWARA Hiroko;SASAKI Shinichi;KASAMI Katsuyu;MIHARA Satoshi;SAITO Kiwamu;HARUYAMA Tomiyoshi - 通讯作者:
HARUYAMA Tomiyoshi
Lattice QCD+QED simulation
晶格 QCD QED 模拟
- DOI:
- 发表时间:
2010 - 期刊:
- 影响因子:0
- 作者:
FUJII Keiko;NAKAMURA Shogo;KATADA Yuki;TORIGOE Yui;ENDO Yuya;YAGISAWA Kazuho;KASAMI Katsuyu;SAITO Kiwamu;SASAKI Shinichi;TAWARA Hiroko;HARUYAMA Tomiyoshi;Taku Izubuchi - 通讯作者:
Taku Izubuchi
NAKAMURA Shogo的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('NAKAMURA Shogo', 18)}}的其他基金
A study on infrared emission of liquid xenon scintillator
液氙闪烁体红外发射研究
- 批准号:
25287055 - 财政年份:2013
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Precise measurement of emission spectrum of liquid xenon scintillator
液态氙闪烁体发射光谱的精确测量
- 批准号:
22540307 - 财政年份:2010
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Monitoring method for seawater pollution in Toyama Bay by analyses of changes in bacterial community structure
细菌群落结构变化分析富山湾海水污染监测方法
- 批准号:
21510030 - 财政年份:2009
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A monitoring method of seawater pollution in coastal zone of Toyama bay by variability analyses of bacterial community structure
基于细菌群落结构变异分析的富山湾沿岸海水污染监测方法
- 批准号:
19510028 - 财政年份:2006
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Precise Measurement of Refractive Index of Liquid Xenon Scintillator
液氙闪烁体折射率的精确测量
- 批准号:
17540240 - 财政年份:2005
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Oil Degrading Ability and Characterization of Oil Degrading Bacteria Isolated from Digestive Gland in Mytilus galloprovincialis.
贻贝消化腺中分离的油降解细菌的油降解能力和特征。
- 批准号:
14580583 - 财政年份:2002
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Oil Degrading Ability and Mechanisms in Oil Degrading Bacterial Groups Isolated from NAKHODKA Oil Spill
NAKHODKA溢油中分离的石油降解菌群的石油降解能力和机制
- 批准号:
12680564 - 财政年份:2000
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
A Tale of No Tail: Sperm Flagella Loss in Mormyrids
无尾的故事:斑鸠鱼精子鞭毛的丧失
- 批准号:
2243230 - 财政年份:2023
- 资助金额:
$ 1.09万 - 项目类别:
Standard Grant
Collaborative Research: Experiments and Modeling of the Fluid Flow of Beating Eukaryotic Flagella
合作研究:真核鞭毛跳动流体流动的实验和建模
- 批准号:
2242095 - 财政年份:2023
- 资助金额:
$ 1.09万 - 项目类别:
Standard Grant
Collaborative Research: Experiments and Modeling of the Fluid Flow of Beating Eukaryotic Flagella
合作研究:真核鞭毛跳动流体流动的实验和建模
- 批准号:
2242096 - 财政年份:2023
- 资助金额:
$ 1.09万 - 项目类别:
Standard Grant
Search and functional analysis of factors that characterize sperm flagella
精子鞭毛特征因子的搜索和功能分析
- 批准号:
22H03214 - 财政年份:2022
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Role of flagella glycosylation in root colonization by a plant growth-promoting bacterium
鞭毛糖基化在植物促生长细菌根部定植中的作用
- 批准号:
572112-2022 - 财政年份:2022
- 资助金额:
$ 1.09万 - 项目类别:
University Undergraduate Student Research Awards
Flagella and TLR5-mediated symbiosis and co-evolution between gut commensal bacteria and host
鞭毛和TLR5介导的肠道共生细菌与宿主之间的共生和共同进化
- 批准号:
22K20576 - 财政年份:2022
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
Toward novel therapies against Lyme disease through the inhibition of lysinoalaine cross-linking in the bacterial flagella.
通过抑制细菌鞭毛中的赖氨酸丙氨酸交联来开发针对莱姆病的新疗法。
- 批准号:
10470087 - 财政年份:2021
- 资助金额:
$ 1.09万 - 项目类别:
Role of flagella glycosylation in plant colonization by a beneficial bacterium
鞭毛糖基化在有益细菌植物定植中的作用
- 批准号:
561802-2021 - 财政年份:2021
- 资助金额:
$ 1.09万 - 项目类别:
University Undergraduate Student Research Awards
General mechanism of the bend formation and propagation revealed by the analysis of insect sperm flagella
昆虫精子鞭毛分析揭示弯曲形成和传播的一般机制
- 批准号:
21H02455 - 财政年份:2021
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Toward novel therapies against Lyme disease through the inhibition of lysinoalaine cross-linking in the bacterial flagella.
通过抑制细菌鞭毛中的赖氨酸丙氨酸交联来开发针对莱姆病的新疗法。
- 批准号:
10663966 - 财政年份:2021
- 资助金额:
$ 1.09万 - 项目类别: