Studies on the mitochondrial nucleoids in Euglena
裸藻线粒体核苷的研究
基本信息
- 批准号:03640583
- 负责人:
- 金额:$ 1.22万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
(1) Concerning the effects of ethidium bromide and chloramphenicol on the mitochondrial nucleoids.Ethidium bromide (EB) stopped the multiplication of cells and decreased their respiratory activity by 55% after treatment for 10 days. Most of the mitochondria became slender with few cristae and some became cup-shaped with stacked cristae. Mitochondrial nucleoids decreased markedly in number after treatment with EB for more than 2 days. After treatment for 3 days with EB, mitochondrial nucleoids could not be detected in about half of all cells examined. Treatment with CAP for 10 days reduced the respiratory activity by 47%. CAP did not decrease the number of mitochondrial nucleoids but it increased the number of cristae and the volume of mitochondria.(2) Concerning the detection of DNA in the nucleoids of chloroplasts and mitochondria by immunoelectron microscopy.Nucleoids in chloroplasts appeared to be composed of twisted threads of 50-70nm in diameter. The twisted threads were entangled to form thicker nodes of 100-200nm in diameter. Most nucleoids in mitochondria were spherical or ovoid, 70-130nm in diameter. Nucleoids both in chloroplasts and in mitochondria contained cores with which DNA threads were in tight contact. These apparent structures of nucleoids were very different from those previously observed by conventional electron microscopy.
(1)溴化乙锭和氯霉素对线粒体类核的影响,溴化乙锭(EB)处理10天后,细胞增殖停止,呼吸活性降低55%。线粒体大多数变细,嵴少,部分呈杯状,嵴堆叠。EB处理2天以上,线粒体类核细胞数量明显减少。在用EB处理3天后,在大约一半的所有检查的细胞中不能检测到线粒体类核。用CAP治疗10天使呼吸活动减少47%。CAP不减少线粒体类核的数量,但增加线粒体嵴的数量和体积。(2)关于叶绿体和线粒体类核DNA的免疫电镜检测,叶绿体类核似乎是由直径为50- 70 nm的螺旋线组成。将扭曲的线缠绕以形成直径为100- 200 nm的较厚的节点。线粒体内的类核多为球形或卵圆形,直径70- 130 nm。叶绿体和线粒体中的类核都含有核心,DNA线与核心紧密接触。这些明显的结构的类核是非常不同的,从那些以前观察到的常规电子显微镜。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
UEDA,K.and NONAKA,M.: "Division of chloroplasts in a green alga,Pedias trum duplex." Annales of Botany. 69. 113-118 (1992)
UEDA,K. 和 NONAKA,M.:“绿藻中叶绿体的分裂,Pedias trum duplex。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Chida,Y.and Ueda,K.: "Detection of actin on organelles of Trebouxia po tteri,in particular on the surface of lysosomes." Protoplasma. 171. 28-33 (1992)
Chida, Y. 和 Ueda, K.:“Trebouxia potteri 细胞器上肌动蛋白的检测,特别是溶酶体表面上的肌动蛋白。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
CHIDA,Y.and UEDA,K.: "Changes in the lysosome structure during the formation of zoospores in Trebouxia potteri." Protoplasma. 171. 19-27 (1992)
CHIDA,Y. 和 UEDA,K.:“Trebouxia potteri 游动孢子形成过程中溶酶体结构的变化。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
CHIDA,Y.and UEDA,K.: "Division of chloroplasts in a green alga,Trebouxia potteri." Annales of Botany 67. 67. 435-442 (1991)
CHIDA,Y. 和 UEDA,K.:“绿藻 Trebouxia potteri 中叶绿体的分裂。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hayashi, Y. and Ueda, K.: "Effects of ethidium bromide and chloramphenicol on the mitochondrial nucleoids in Euglena gracilis." Physiol. Plant.86. 57-62 (1992)
Hayashi, Y. 和 Ueda, K.:“溴化乙锭和氯霉素对眼虫线粒体核苷的影响。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
UEDA Katsumi其他文献
UEDA Katsumi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
相似海外基金
Development of skin-permeable cancer therapeutics targeting mitochondria DNA
开发针对线粒体 DNA 的皮肤渗透性癌症疗法
- 批准号:
21K14756 - 财政年份:2021
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
NETs including oxidative mitochondria DNA might make worse the autoimmune-inflammation in CGD
包含氧化线粒体 DNA 的 NET 可能会使 CGD 患者的自身免疫炎症恶化
- 批准号:
17K10006 - 财政年份:2017
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of novel therapeutics targeting mitochondria DNA
开发针对线粒体 DNA 的新型疗法
- 批准号:
15K19387 - 财政年份:2015
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Mechanisms of meiotically programmed destruction of mitochondria DNA
线粒体 DNA 减数分裂程序性破坏的机制
- 批准号:
466040-2014 - 财政年份:2014
- 资助金额:
$ 1.22万 - 项目类别:
University Undergraduate Student Research Awards
Analysis of higher-order structures of mitochondria DNA using the enChIP technology
使用 enChIP 技术分析线粒体 DNA 的高阶结构
- 批准号:
26650059 - 财政年份:2014
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Clarification of the role of regulatory mechanism of mitochondria DNA in heart and the application to treatment of heart failure
阐明线粒体DNA在心脏中的调控机制及其在心力衰竭治疗中的应用
- 批准号:
26461129 - 财政年份:2014
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
An analysis of cell lineage for breast cancer by gene alteration of mitochondria DNA D-loop region
通过线粒体 DNA D 环区域的基因改变分析乳腺癌细胞谱系
- 批准号:
24590433 - 财政年份:2012
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analyzing how defects of Mitochondria DNA affect oocyte maturation.
分析线粒体 DNA 缺陷如何影响卵母细胞成熟。
- 批准号:
21592117 - 财政年份:2009
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of hepatocarcinogenesis by mitochondria DNA analysis and its clinical signifianoe
线粒体DNA分析肝癌发生机制及其临床意义
- 批准号:
15591430 - 财政年份:2003
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research on the Yayoi people and their society based on the mitochondria DNA analysis
基于线粒体DNA分析的弥生人及其社会研究
- 批准号:
09440284 - 财政年份:1997
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)