Mechanism of hepatocarcinogenesis by mitochondria DNA analysis and its clinical signifianoe

线粒体DNA分析肝癌发生机制及其临床意义

• 批准号: 15591430
• 负责人: KUBO Shoji
• 金额: $ 1.66万
• 依托单位: Osaka City University graduate School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591430/
• 关键词: Hepatocellular carcinoma; Mitochondria DNA; Hepatic fibrosis; Chronic hepatitis C; Intrahepatic cholangioma; Liver resection; ミトコンドリアDNA

The patterns of point mutation and deletion of mitochondria DNA in cancerous tissues and noncancerous hepatic tissues in patients with hepatocellular carcinoma and without the carcinoma. Although the number of such changes were limited in normal liver, the number of the changes were significantly higher in the carcinoma tissues and noncancerous hepatic tissues in patients with hepatocellular carcinoma. The number of the changes increased as the differentiation. Persistent chronic hepatitis C affects the mitochondria DNA because the number of the changes increased as the progression of hepatitis. Although the degree of hepatic fibrosis was not correlated with the changes of point mutation and deletion of mitochondria DNA, the prevalence of muticentric carcinogenesis was correlated with the changes. These findings suggest that the number of the changes was correlated with the potential of hepatocarcinogenesis. The prevalence of multicentric carcinogenesis was correlated with platelet count In patients with low platelet count less than 10^4/mm^3, it is necessary to pay attention of the development of multicentric carcinogenesis. Some patients with intrahepatic cholangiocarcinoma were infected with hepatitis C, indicating that hepatitis C infection. affect the development of intrahepatic cholangiocarcinoma. The evaluation of the potential of hepatocarcinogenesis is useful to detect the carcinoma.

肝细胞癌患者癌组织和非癌肝组织线粒体DNA点突变和缺失的研究虽然在正常肝脏中这种改变的数目有限，但在肝细胞癌患者的癌组织和非癌肝组织中，这种改变的数目明显较多。变化的次数随着分化程度的增加而增加。持续性慢性丙型肝炎会影响线粒体DNA，因为改变的数量随着肝炎的进展而增加。尽管肝纤维化程度与线粒体DNA点突变和缺失的变化无关，但多中心性癌变的发生率与这些变化有关。这些发现表明，这些变化的数量与肝癌发生的可能性有关。低血小板低于104/mm~3者，多中心癌变的发生率与血小板计数呈正相关，应重视多中心癌变的发生发展。部分肝内胆管细胞癌患者感染丙型肝炎，提示丙型肝炎感染。影响肝内胆管细胞癌的发生发展。评估肝细胞癌发生的可能性有助于发现该肿瘤。

项目成果

Appropriate surgical management of small hepatocellular carcinoma in patients infected with hepatitis C virus.

丙型肝炎病毒感染患者的小肝细胞癌的适当手术治疗。

• 发表时间: 2003
• 期刊: World J Surg 27
• 作者: Kubo S;et al.
• 通讯作者: et al.

A mixed hepatocellular carcinoma and cholangiocarcinoma : dual expression of biliary-type cytokeratin and hepatocyte specific marker.

混合性肝细胞癌和胆管癌：胆管型细胞角蛋白和肝细胞特异性标记物的双重表达。

• 发表时间: 2004
• 期刊: Hepatogastroenteorlogy 51
• 作者: Tanaka S;Kubo S;et al.
• 通讯作者: et al.

Kubo S, et al.: "Multicentric occurrence of hepatocellular carcinoma in patients with a somatic mutation of mitochondorial DNA and hepatitis C virus"Hepatology Research. 25. 78-82 (2003)

Kubo S 等人：“线粒体 DNA 体细胞突变和丙型肝炎病毒患者多中心发生肝细胞癌”肝病学研究。

Kubo S, et al.: "Appropriate surgical management of small hepatocellular carcinoma in patients infected with hepatitis C virus"World Journal of Surgery. 27. 437-442 (2003)

Kubo S 等人：“丙型肝炎病毒感染患者小肝细胞癌的适当手术治疗”世界外科杂志。

Clinicopathologic characteristics of small intrahepatic cholangiocarcinomas of mass-forming type

肿块型肝内小胆管癌的临床病理特征

• 发表时间: 2004
• 期刊: Hepatology Research 29
• 作者: Kubo S;et al.
• 通讯作者: et al.