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Studies on humoral regulation of gonadal differentiation

性腺分化的体液调节研究

基本信息

批准号：
03640623
负责人：
NOUMURA Tetsuo
金额：
$ 1.22万
依托单位：
Saitama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

In mammals, the direction of sex differentiation of primordial gonad into a testis or an ovary can be controlled by a gene located on the Y-chromosome, but the factors involved in gene-regulatory cascade leading to morphological testicular differentiation is not clear. The mammalian gonad is a complex structure formed by diverse cell lineages, the germ and the somatic cells. Testicular differentiation in rats begins with Sertoli cell differentiation on 13 day of fetal age, followed by seminiferous cord formation and Leydig cell differntiation. This study examined by three different approaches to elucidate the roles of growth factors (TGF-beta, inhibit, EGF, FGF, IGF-II) in rat gonadal differentiation and development during perinatal period, because these growth factors playd a wide range of roles for embryonic development as well as many endocrine, paracrine and autocrine functions.1. Localization and expression patterns of these growth factors in developing rat gonads throughout the perinatal period, from just before the onset of gonadal sex differentiation to postnatal weaning age, were examined immunohistochemically. Each growth factor was expressed in sex-, cell-, and stage-specific manners.2. Effects of these growth factors on developing rat gonads were examined morphologically in indifferent gonads from 13 day fetal rats which were cultured for 4 days in growth factor or anti-serum containing medium. Inhibin suppressed both the seminiferous cord formation and the germ cell meiosis.3. The somatic cells in experimentally masculinized fetal rat ovaries, which were transplanted either close to co-grafting fetal tests beneath the renal capsule of the castrated adult or into the adult testicular subcapcular site, showed similar seminiferous cord-like structure formation and immunohistochemical expression of growth factors to those in the co-grafted fetal testes.

在哺乳动物中，原始性腺向睾丸或卵巢的性别分化方向可以由位于Y染色体上的基因控制，但导致睾丸形态分化的基因调控级联反应的因素尚不清楚。哺乳动物性腺是由生殖细胞和体细胞组成的复杂结构。大鼠睾丸的分化始于胎龄13天的支持细胞分化，随后是生精索的形成和间质细胞的分化。本研究通过三种不同的方法来研究生长因子（TGF-β、Inhibit、EGF、FGF、IGF-II）在围产期大鼠性腺分化和发育中的作用，因为这些生长因子在胚胎发育中起着广泛的作用，同时还具有多种内分泌、旁分泌和自分泌功能.这些生长因子的定位和表达模式，在整个围产期发育大鼠性腺，从性腺性别分化的发病前到出生后断奶年龄，进行了化学检查。每种生长因子都以性别、细胞和阶段特异性的方式表达。将13天胎鼠的不同性腺在含生长因子或抗血清的培养基中培养4天，从形态学上观察这些生长因子对发育中的大鼠性腺的影响。抑制素抑制生精索的形成和生殖细胞的减数分裂.在实验雄性化胎鼠卵巢的体细胞，这是接近共同移植胎儿测试下的肾包膜的阉割成人或成人睾丸囊下网站，表现出类似的生精索样结构的形成和免疫组化表达的生长因子的共同移植胎儿睾丸。