Study of sensory mechanisms from the pelvic viscera

盆腔脏器感觉机制的研究主要集中在两个方面。应用免疫组织化学技术研究了单个靶器官初级感觉神经节神经元的神经递质鉴定。在这些神经元中发现了P物质、CGRP、亮氨酸脑啡肽、VIP。VIP高度位于子宫颈传入神经元。第二个主题是传入终端的激活机制。膀胱感染后，出现尿频和疼痛的膀胱炎，这可能是由于感染过程中感觉终端的改变。研究了炎症过程中白细胞释放的白细胞介素1 β (il1 β)。il - 1 β降低了排尿反射的阈值体积，降低了背根记录放电的阈值压力。将膀胱内溶液改变为较低的PH值（<3.5），可以降低C纤维的放电阈值压力。相比之下，Adelta纤维未见变化。此外，62%的c -纤维无反应者在低pH溶液后发生放电。较高的pH值并没有改变烧灼的发生。这些数据表明，来自骨盆器官的含有许多肽能递质的初级传入神经元和膀胱粘膜的神经末梢在给予il - 1 β或将膀胱内溶液改变为较低的ph后变得敏感，从而可能在正常情况下沉默的c -纤维在炎症期间变得活跃，并可能导致病理症状。

Study of sensory mechanisms from the pelvic viscera has been focused on two parts. Identify neurotransmitter at primary sensory ganglion neurons was studied for individual target organs using immunohistochemical techniques. Substance P, CGRP, leucine enkephalin, VIP have been identified in these neurons. VIP was highly located in the uterine cervix afferent neurons. Second topic was the mechanisms of activation of the afferent terminals. Since urinary bladder become infection, so call cystitis has been developed with frequent urination and pain, This could be due to change of sensory terminals during the infection. Interleukin 1beta (IL1beta) which released from white blood cells during the inflammation was studied. IL1beta lowered threshold volume of micturition reflex and lowered the threshold pressure for firing at the dorsal root recording. Changing the intravesical solution to lower PH(<3.5) brought the threshold pressure for firing down in C fiber. In contrast no change was observed in Adelta fibers. Additionaly, 62% of C-fiber none responders for disteition became firing after low pH solution. Higher pH solution did not altered the thereshould or occurrence of firing.These data indicated that primary afferent neurons from the pelvic organs containing many peptidergic transmitters and nerve terminals at bladder mucosa become sensitive following administration of Il1beta or changing intravesical solution to lower pH. Thus might be silent C-fiber in normal become active during inflammation and could be contribute the pathological symptom.