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Study of sensory mechanisms from the pelvic viscera

盆腔内脏感觉机制的研究

基本信息

批准号：
04044145
负责人：
KAWATANI Masahito
金额：
$ 5.38万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04044145/
关键词：
Urinary Bladder pH Uterus neuropeptides inflammation sensory receptors インターロイキン1 神経終末 Fura 2 脊髄 NKI受容体

项目摘要

Study of sensory mechanisms from the pelvic viscera has been focused on two parts. Identify neurotransmitter at primary sensory ganglion neurons was studied for individual target organs using immunohistochemical techniques. Substance P, CGRP, leucine enkephalin, VIP have been identified in these neurons. VIP was highly located in the uterine cervix afferent neurons. Second topic was the mechanisms of activation of the afferent terminals. Since urinary bladder become infection, so call cystitis has been developed with frequent urination and pain, This could be due to change of sensory terminals during the infection. Interleukin 1beta (IL1beta) which released from white blood cells during the inflammation was studied. IL1beta lowered threshold volume of micturition reflex and lowered the threshold pressure for firing at the dorsal root recording. Changing the intravesical solution to lower PH(<3.5) brought the threshold pressure for firing down in C fiber. In contrast no change was observed in Adelta fibers. Additionaly, 62% of C-fiber none responders for disteition became firing after low pH solution. Higher pH solution did not altered the thereshould or occurrence of firing.These data indicated that primary afferent neurons from the pelvic organs containing many peptidergic transmitters and nerve terminals at bladder mucosa become sensitive following administration of Il1beta or changing intravesical solution to lower pH. Thus might be silent C-fiber in normal become active during inflammation and could be contribute the pathological symptom.

对盆腔脏器感觉机制的研究主要集中在两个方面。应用免疫组化技术对单个靶器官的初级感觉神经节神经元的神经递质进行了研究。在这些神经元中已鉴定出P物质、CGRP、亮氨酸脑啡肽、VIP。VIP在子宫颈传入神经元中高度定位。第二个主题是传入末梢的激活机制。膀胱感染后，会出现尿频、尿痛等症状，这可能是由于感染时感觉末梢发生变化所致。研究了炎症过程中由白色血细胞释放的白细胞介素1 β（IL 1 β）。IL 1 β降低排尿反射阈值和背根放电阈值。改变膀胱灌注液pH值（<3.5）可使C纤维放电阈压降低。相比之下，在Δ纤维中没有观察到变化。此外，62%的C-纤维无反应者在低pH溶液后开始放电。结果表明，IL 1 β或改变膀胱灌注液的pH值，可使含有多种肽能递质的盆腔器官初级传入神经元和膀胱粘膜神经末梢变得敏感，从而可能使正常情况下沉默的C纤维在炎症过程中变得活跃，并可能参与病理症状的发生。