Mechanism of recovery of autonomic function in the pelvic ganglia following pelvic nerve transection

用电压敏感染料和光电二极管阵列检测系统研究了大鼠盆神经切断后盆神经节的可塑性。实验前2~4周行单侧盆神经横断术。在用Nembutal深度麻醉后，取出两侧盆神经节，用泰氏液孵育。用电压敏感染料暴露神经节30min，用Deltalon 1700(富士胶片公司，东京)测量其亮度。利用计算机系统对二值化信号进行分析。当亮度变化小于对照组的0.01%时，颜色保持为绿色。黄色表示变化0.05%，红色表示变化超过0.01%。屏幕上显示256个色差。对照组盆神经节在连续测量期间亮度没有变化，计算机演示显示为绿色。施氯化钾(50 mm~150 mm)在0.9s内变红，变绿(对照)。红区面积随KCl剂量的增加而增加。外加ACh(3.3-50µM)也使颜色变为与KCl不完全匹配的红色(看起来比KCl小，但独立)。在分散的神经节记录到自发放电，放电开始于神经节内的少数点，并移至周围区域。放电每隔0.3-0.5s发生一次，对河豚毒素敏感。应用KCl和ACh也能激活神经节细胞。KCl兴奋阈值与对照组相同，而ACh仅为对照组的1/5~1/10，提示盆神经切断后形成了新的兴奋网络系统，这可能有助于通过少量的腹下神经输入兴奋神经节内的大部分细胞。

Plasticity of the pelvic ganglia in the rat following pelvic nerve transection has been studied using voltage sensitive dye and photodiode array detecting system. Unilateral transection of the pelvic nerve was performed 2-4 weeks prior to the experiments. After deeply anesthetized with nembutal, both side of the pelvic ganglia were removed out and incubated with Tyrode's solution. The ganglia were expose with voltage sensitive dye for 30 min and measuring its brightness using Deltalon 1700 (Fuji Film Co, Tokyo). Diditalized signal were analyzed with computer system. When the brightness change were than 0.01% of the control, the color stayd in green. Yellow showed 0.05% change and red showed more than 0.01% change. 256 color difference will be presented on the screen.In control pelvic ganglia, the brightness did not change over the period of continuous measurements and computer demonstration showed green. Application of KCl(50mM - 150mM) changed the color to red and the color return to green(control) in 0.9s. The red area increased by KCl doses. Application ACh(3.3muM - 50muM) also changed the color to red which is not completely match with KCl(seems less area than KCl, but independent). The excitation return to control in 2S.In the decentralized ganglia, spontaneous firing was recorded, The firing started few point in the ganglia and moved to the surrounded area. The firing occurred every 0.3 - 0.5s, and this is sensitive to tetrodotoxin. Application of KCl and ACh also activate the ganglia cells. Threshold of KCl excitation as same as control but ACh was 1/5 - 1/10 of the control.These data suggest new excitation network system was developed following pelvic nerve transection, and this could contribute to excite most of cells in the ganglia by small input from hypogastric nerve.