Molecular mechanisms of sensory svstem from urinary bladder

膀胱感觉系统的分子机制

• 批准号: 08044239
• 负责人: KAWATANI Masahito
• 金额: $ 5.31万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044239/
• 关键词: urinary bladder; phasic neuron; interleukin 1beta; prostaglandin; spinal cord; parasympathetic preganglionic neurons; サイレント線維; 感覚線維; 神経伝達物質; CRF; グルタメイト

Autonomic control of urinary bladder has been studied. Sensory nerve ending was activated primarily by distention of the bladder wall. Chemical irritation using acetic acid and inflammation related substance interluikin 1beta (IL1beta) changed the threshold for micturition reflex by activating the high threshold fibers. Prostaglandin E (PGE) activated silent C fiber unit and changed threshold of high threshold fiber. Activity was not changed in low threshold fiber. The effect of IL1 beta and PGE were abolished by indomethacin. High threshold fibers which is not used in normal micturition reflex was activated inflammation related substances and increased the frequency of micturition and reduced the capacity of the urinary bladder.Parasympathetic preganglionic neurons (PGN) were divided into tonic PGN and phasic PGN on the basis of firing properties during prolonged (300ms) depolarizing current pulses. 4-Aminopyridine (4-AP) reduced the threshold for spike activation in tonic and phasic PGN.4-AP unmasked tonic firing in phasic PGN and increased firing frequency in tonic PGN.These data indicated that two types of PGN were present and may have different innervation to the targets in the pelvic viscera.

膀胱的自主控制已经被研究。感觉神经末梢主要由膀胱壁的扩张激活。用醋酸和炎症相关物质白细胞介素1 β（IL 1 β）进行化学刺激，通过激活高阈值纤维改变排尿反射阈值。前列腺素E（PGE）激活沉默C纤维单位，改变高阈值纤维的阈值。低阈值纤维的活性没有改变。消炎痛可阻断IL 1 β和PGE的作用。本研究根据副交感神经节前神经元（PGN）在长时间（300 ms）去极化电流脉冲作用下的放电特性，将其分为紧张性PGN和相位性PGN。4-氨基吡啶（4-AP）可降低强直性PGN和相性PGN的放电阈值，揭示相性PGN的强直性放电，增加强直性PGN的放电频率。

项目成果

Kawatani M,Suzuki T,de Groat WC.: "Corticotropin releasing factor-like immuroreactivity in afferent projections to the sacral spinalcord of the cat." Auton Nerve Syst.61. 218-226 (1996)

Kawatani M、Suzuki T、de Groat WC.：“猫骶脊髓传入投射中促肾上腺皮质激素释放因子样免疫反应性。”

河谷 正仁: "仙髄における骨盤神経求心性神経終末とその伝達物質-脊髄と排尿機能-" 排尿障害プラクティス. 4. 365-370 (1996)

Masahito Kawatani：“骨盆神经传入神经末梢及其在骶脊髓中的传递器 - 脊髓和泌尿功能”泌尿障碍实践。

河谷 正仁: "仙髄の排尿中枢とオヌフ核-脊髄と排尿機能-" 排尿障害プラクティス. 5. 192-198 (1997)

Masahito Kawatani：“骶脊髓排尿中枢和 Onuf 核 - 脊髓和排尿功能 -” 泌尿疾病实践 5. 192-198 (1997)。

Kawatani M,Suzuki T,de Groat WC: "Corticotropin releasing factor like immuroreactivity in afferent projections to the sacral spinal cord of the cat." Auton Nerve Syst. 61. 218-226 (1996)

Kawatani M、Suzuki T、de Groat WC：“促肾上腺皮质激素释放因子，例如猫骶脊髓传入投射中的免疫反应性。”

Miura A Kawatani M: "Effects of diode laser irradiation on sensory ganglion cells from the cat." Pain Res. 11. 175-183 (1996)

Miura A Kawatani M：“二极管激光照射对猫感觉神经节细胞的影响。”