Mass Spectrometric Approach to the Study of Biological Transducers

该项目的目的是通过质谱分析阐明与生物通信系统（如视觉或生物发光）相关的分子过程，与电子通信大学（UEC）的ohashi教授小组以及俄克拉何马大学（OU）生物化学系的松本教授和化学系的schmitz教授的研究小组合作。三组之间的相互交流如下：1992年9月，松本先生访问了UEC， 10月，tsujimoto先生访问了OU。1993年1月至5月，UEC研究生Azegani与Schmitz课题组一起从海洋中提取几种细胞毒性化合物，9月，UEC研究生kinumi与Matsumoto课题组一起制备Phosrestin相关的寡肽，11月，ohashi和Hirano访问OU进行研讨会。1994年，Jakson（3月），Schmitz（6月），Matsumoto（7月）访问了UEC， 8月，hirano和Niwa访问了Scripps海洋学研究所，UCSD，OU和Washington university的Friday Harbor收集了生物发光生物，11月，kinumi临时访问了UEC，建立了质谱方法来阐明phorestin的翻译后修饰。UEC组尝试用质谱法来阐明aequorin生物发光的活性中心，而OU组主要关注于阐明Phosrestin的翻译后修饰，Phosrestin是果蝇光感受器细胞在体内进行光诱导可逆磷酸化，并通过质谱法确定体内磷酸化位点。三个小组的合作进展顺利，Kinumi、jackson、Kubota、Ohashi和Matsumoto的题为“蝇眼中磷素1的磷酸化和n端乙酰化”的联合论文将在1995年asm会议上发表。

The aim of this project is to elucidate the molecular processes associated with biological communication systems such as vision or bioluminescence by means of mass spectrometry in collaboration with prof.Ohashi's group of the University of Electro-Communications (UEC) and the research groups of Prof.Matsumoto of Biochemistry Dept., and Prof.Schmitz of Chemistry Dept.of Oklahoma University (OU). The mutual exchanges which have been done between the three groups are as follows ; In 1992, in Sep.Matsumoto visited UEC,in Oct.Tsujimoto visited OU.In 1993 from Jan.to May, Azegani, Graduate student of UEC,joined Schmitz's group to extract several cytotoxic compounds from marine sources, in Sep.Kinumi, Graduate student of UEC,joined Matsumoto's group to prepare oligopeptides related to Phosrestin, in Nov.Ohashi and Hirano visited OU to give a scminar. In 1994, Jakson (in Mar), Schmitz (in Jun.), Matsumoto (in Jul.) visited UEC,in Aug.Hirano and Niwa visited Scripps Institution of Oceanography, UCSD,OU and Friday Harbor of Washington Univ.to collect bioluminescent organism, in Nov.Kinumi temporally visited UEC to establish mass spectrometric methods for elucidating the post-translational modification of phosrestin.The UEC group has attempted to use mass spectrometry to elucidate the active center of aequorin bioluminescence, while the OU groups have mainly concerned elucidating the post-translational modification of Phosrestin, which is in fly photoreceptor cells undergoing light-induce reversible phosphorylation in vivo, and determining the in vivo phosphorylation site by mass spectrometry. The collaboration of the three groups have proceeded smoothly and the joint paper entitled "Phosphorylation and N-terminal acetylation of phosrestin 1 in fly eyes" by Kinumi, Jachson, Kubota, Ohashi, and Matsumoto will be presented at the 1995 ASMS meeting.