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Mass Spectrometric Approach to the Study of Biological Transducers

研究生物传感器的质谱方法

基本信息

批准号：
04045024
负责人：
OHASHI Mamoru
金额：
$ 4.29万
依托单位：
The University of Electro-Communications
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

The aim of this project is to elucidate the molecular processes associated with biological communication systems such as vision or bioluminescence by means of mass spectrometry in collaboration with prof.Ohashi's group of the University of Electro-Communications (UEC) and the research groups of Prof.Matsumoto of Biochemistry Dept., and Prof.Schmitz of Chemistry Dept.of Oklahoma University (OU). The mutual exchanges which have been done between the three groups are as follows ; In 1992, in Sep.Matsumoto visited UEC,in Oct.Tsujimoto visited OU.In 1993 from Jan.to May, Azegani, Graduate student of UEC,joined Schmitz's group to extract several cytotoxic compounds from marine sources, in Sep.Kinumi, Graduate student of UEC,joined Matsumoto's group to prepare oligopeptides related to Phosrestin, in Nov.Ohashi and Hirano visited OU to give a scminar. In 1994, Jakson (in Mar), Schmitz (in Jun.), Matsumoto (in Jul.) visited UEC,in Aug.Hirano and Niwa visited Scripps Institution of Oceanography, UCSD,OU and Friday Harbor of Washington Univ.to collect bioluminescent organism, in Nov.Kinumi temporally visited UEC to establish mass spectrometric methods for elucidating the post-translational modification of phosrestin.The UEC group has attempted to use mass spectrometry to elucidate the active center of aequorin bioluminescence, while the OU groups have mainly concerned elucidating the post-translational modification of Phosrestin, which is in fly photoreceptor cells undergoing light-induce reversible phosphorylation in vivo, and determining the in vivo phosphorylation site by mass spectrometry. The collaboration of the three groups have proceeded smoothly and the joint paper entitled "Phosphorylation and N-terminal acetylation of phosrestin 1 in fly eyes" by Kinumi, Jachson, Kubota, Ohashi, and Matsumoto will be presented at the 1995 ASMS meeting.

本项目的目的是与电子通信大学（UEC）的大桥教授小组和生物化学系的松本教授的研究小组合作，通过质谱法阐明与视觉或生物发光等生物通信系统相关的分子过程。和美国俄克拉荷马州大学化学系的Schmitz教授（俄）。三个研究组之间的相互交流如下：1992年9月，松本访问了UEC，10月，Tsujimoto访问了UEC。1993Jan.to，UEC研究生Azegani加入Schmitz的研究组，从海洋资源中提取几种细胞毒性化合物，9月，UEC研究生Kinumi加入松本的研究组，制备与Phosrestin相关的寡肽，11月，大桥和平野访问了日本，并进行了演讲。1994年，Jakson（3月），Schmitz（6月），松本（7月）2010年8月，平野和丹羽访问了UECUniv.to年11月，木见访问了UEC，建立了用于阐明水母发光蛋白翻译后修饰的质谱方法。UEC组试图利用质谱来阐明水母发光蛋白的活性中心，而NH3组主要关注于阐明Phosrestin的翻译后修饰，其在果蝇感光细胞中进行体内光诱导可逆磷酸化，并通过质谱法确定体内磷酸化位点。这三个小组的合作进展顺利，Kinumi、Jachson、Kubota、Ohashi和松本的题为“Phosphorylation and N-terminal acetylation of Aestrestin 1 in fly eyes”的联合论文将在1995年ASMS会议上发表。