Improving the efficiency of CRISPR gene editing in cells

提高细胞中CRISPR基因编辑的效率

• 批准号: LP210200125
• 负责人: Prof Kate Quinlan
• 金额: $ 29.75万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP210200125
• 关键词: Improving; efficiency; CRISPR; gene; editing

Human red blood cells are well-characterised and the globin gene locus is a model system for the study of gene regulation. Gene editing technologies and delivery tools are evolving rapidly and the globin gene locus is the perfect model for gene editing optimisation. This collaboration between UNSW Sydney and CSL aims to bring together our combined expertise and new technologies to develop an optimal platform for genetic modification in a red blood cell line. Simultaneously, this project aims to generate fundamental insights into mechanisms of human gene regulation. The technological and biological outcomes of this project will be of benefit for future gene editing applications.

人类红细胞具有很好的特性，珠蛋白基因座是研究基因调控的模式系统。基因编辑技术和传递工具正在迅速发展，珠蛋白基因位点是基因编辑优化的完美模型。新南威尔士大学悉尼分校和CSL之间的这项合作旨在将我们的专业知识和新技术结合在一起，开发一个在红细胞系中进行基因改造的最佳平台。同时，该项目旨在对人类基因调控的机制产生基本的见解。该项目的技术和生物学成果将有利于未来的基因编辑应用。