权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Biomimetic peptide-polymer nanostructured materials

仿生肽聚合物纳米结构材料

基本信息

批准号：
5251194
负责人：
Professor Dr. Markus Biesalski
金额：
--
依托单位：
Fachgebiet Makromolekulare Chemie
依托单位国家：
德国
项目类别：
Independent Junior Research Groups
财政年份：
2000
资助国家：
德国
起止时间：
1999-12-31 至 2011-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/5251194?language=en
关键词：
Biomimetic peptide polymer nanostructured materials

项目摘要

In the last years a novel approach to improve the bioactivity of surfaces was developed in the group of Prof. M. Tirrell. Surfaces modified with peptide-amphiphile molecules consisting of lipid tail and a peptide-sequence, which promotes cell-adhesion and spreading, were prepared and characterized. The lipidation of a collagen peptide-sequences induces a self-assembling of the peptide-amphiphiles into a triple-helical conformation in solution, similar to the structure of the collagen in vivo. It was shown that thin layers of the peptide-amphiphiles in which the lipid tails were adsorbed onto solid substrates promote cell-adehsion and cell-spreading. In the proposed project ... research on the synthesis of peptide-amphiphiles as well as on the improvement of bioactivity (cell-adehsion, cell-spreading) will be followed. Using unsaturated as well as "short" fatty acid alcohols for the lipidation of distinct peptide-sequences peptide amphiphiles will be generated and characterized which should give more "flexible" layers at solid surfaces. Furthermore, to improve the stability of the layers, polymerizable lipids which can be polymerized in the lipid film will be used. Additionally the peptide-amphiphile layers will be covalently attached to solid surfaces to improve long-term stability of the films. Finally cell-adhesion as well as cell-spreading will be investigated on the pre-formed peptideamphiphile modified surfaces.

在过去的几年里，M。提洛表面修饰的肽两亲分子组成的脂质尾巴和肽序列，促进细胞粘附和蔓延，制备和表征。胶原蛋白肽序列的脂化诱导肽-两亲物在溶液中自组装成三螺旋构象，类似于体内胶原蛋白的结构。结果表明，薄层的肽-两亲物，其中脂质尾部吸附到固体基质上，促进细胞粘附和细胞铺展。在拟议的项目中…随后将研究肽-两亲物的合成以及生物活性（细胞粘附、细胞铺展）的改善。使用不饱和以及“短”脂肪酸醇的不同的肽序列的脂化肽两亲物将产生和表征，这应该给更多的“灵活”层在固体表面。此外，为了提高层的稳定性，将使用可在脂质膜中聚合的可聚合脂质。此外，肽-两亲物层将共价连接到固体表面以改善膜的长期稳定性。最后，将研究预形成的肽两亲物修饰的表面上的细胞粘附以及细胞铺展。