Novel preservation solution and ADF/hThioredoxine-a radical scavenger

肺移植已被确立为终末期肺部疾病患者的成功治疗选择。然而，由于缺乏合适的供体肺，限制了这种方法的广泛应用。为了克服这一限制，我们开发了一种新的细胞外或细胞内类型的溶液，ET-Kyoto（ET-K）或IT-Kyoto（IT-K）溶液，其中含有4.1%的海藻糖，葡萄糖酸盐和羟乙基淀粉（HES）。我们的结果表明，用ET-Kyoto溶液保存20小时的移植肺的功能明显优于用IT-K或EC溶液保存的肺。我们的结果还表明，用ET-Kyoto溶液保存30小时的移植肺的功能明显优于用UW溶液保存的肺。肺移植后会发生缺血再灌注损伤，预防这种损伤是肺保存的重要内容。众所周知，活性氧主要与缺血再灌注损伤的发生有关。人硫氧还蛋白/成人T细胞白血病衍生因子（ADF）由104个氨基酸组成，具有两个半胱氨酸，被认为是自由基清除剂。在这份报告中，我们研究了ADF对缺血再灌注损伤的抑制作用，作为一种自由基清除剂在大鼠和狗。

Lung transplantation has been setablished as a successful therapeutic option for patients with end-stage lung diseases. However, the the wide spread application of this proedure is limited by the shortage of suitable donor lungs. To overcome this limitation, we developed a new extra or intracellular type solution, ET-Kyoto (ET-K) or IT-Kyoto (IT-K) solution, which contains 4.1% trehalose, gluconate, and hydroxyethyl starch (HES). Our results demonstrated that the function of the transplanted lung preserved with the ET-Kyoto solution for 20 hours was significantly better than those of lungs with IT-K or the EC solution. Our results also showed that the function of the transplanted lung preserved with the ET-Kyoto solution for 30 hours was significantly better than that of lungs with the UW solution. Ischemia-reperfusion injury occurs after lung transplantation and prevention of this injury is very important in lung preservation. It is widely known that active oxygen species are associated mainly with the occurrence of ischemia-reperfusion injury. Human thioredoxin/Adult T-Cell Leukemic-derived Factor (ADF) is composed of 104 amino acids with the two Cys and considered as radical scavenger. In this report, we investigated the inhibitory effect of ADF against ischemia-reperfusion injury as a radical scavenger in rats and dogs.