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Novel preservation solution and ADF/hThioredoxine-a radical scavenger

新型保存液和ADF/h硫氧还蛋白-自由基清除剂

基本信息

批准号：
04404055
负责人：
WADA Hiromi
金额：
$ 10.56万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04404055/
关键词：
Lung transplantation Lung preservation Trehalose ET-Kyoto solution Ischemia-reperfusion lung injury Adult T Cell Leukemia derived Factor Human thioredoxin Radical scavenger 肺再灌流障害 ADF Thioredoxin 虚血再灌流障害臓器保存

项目摘要

Lung transplantation has been setablished as a successful therapeutic option for patients with end-stage lung diseases. However, the the wide spread application of this proedure is limited by the shortage of suitable donor lungs. To overcome this limitation, we developed a new extra or intracellular type solution, ET-Kyoto (ET-K) or IT-Kyoto (IT-K) solution, which contains 4.1% trehalose, gluconate, and hydroxyethyl starch (HES). Our results demonstrated that the function of the transplanted lung preserved with the ET-Kyoto solution for 20 hours was significantly better than those of lungs with IT-K or the EC solution. Our results also showed that the function of the transplanted lung preserved with the ET-Kyoto solution for 30 hours was significantly better than that of lungs with the UW solution. Ischemia-reperfusion injury occurs after lung transplantation and prevention of this injury is very important in lung preservation. It is widely known that active oxygen species are associated mainly with the occurrence of ischemia-reperfusion injury. Human thioredoxin/Adult T-Cell Leukemic-derived Factor (ADF) is composed of 104 amino acids with the two Cys and considered as radical scavenger. In this report, we investigated the inhibitory effect of ADF against ischemia-reperfusion injury as a radical scavenger in rats and dogs.

肺移植已被确立为终末期肺部疾病患者的一种成功的治疗选择。然而，由于缺乏合适的供肺，该技术的广泛应用受到了限制。为了克服这一局限性，我们开发了一种新的胞外或胞内型溶液，ET-京都(ET-K)或IT-京都(IT-K)溶液，其中包含4.1%的海藻糖、葡萄糖酸盐和羟乙基淀粉(HES)。结果表明，ET-京都液保存20h的移植肺功能明显优于IT-K或EC液保存的移植肺。ET-京都液保存30h的移植肺功能明显好于UW液保存的移植肺。肺移植后会发生缺血再灌注损伤，预防缺血再灌注损伤对肺保存具有重要意义。众所周知，活性氧主要与缺血再灌注损伤的发生有关。人硫氧还蛋白/成人T细胞白血病衍生因子(ADF)由104个氨基酸组成，具有清除自由基的功能。在本报告中，我们研究了ADF作为自由基清除剂对大鼠和狗的缺血再灌注损伤的抑制作用。