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Potency of calcification in aeticular chondrocyte

软骨细胞钙化能力

基本信息

批准号：
04404060
负责人：
OKA Masanori
金额：
$ 7.04万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04404060/
关键词：
Chondrocytes Repair of joint Calcification Hyaline cartilaqe Mesenchymal tissue 未分化問葉細織

项目摘要

The main purpose of this study is how to repair damaged joint sunace. For this purpose, we perlormed three kinds of experiments. First, isogenic and allogenic chondrocytes cultured in collagen gel were transplanted into osteo-chondral defects made on patellar groove in inbred strain rats. Till 12 months postperatively, the healing behaviors were histologically observed, including immunohistochemistry to detect type 2 collagen.Long term observation showed that allografted defects healed as successfully as isografted one, although subchondral bone formation was delayd in the foemer at the 12 weeks stage. On the other hand, no control defects which had been implanted with only collagen gel or left open, healed with hyaline carfilage. ln the second experiment, to investigate difference in potent calcification of chondrocytes depending on the cell layr, we performed autograft of arficular carfilage in situ and also implanted up-side-down the graft. As a result, aimost complete repair was obtained in in-situ grafted group, whereas the joint surface were covered by fibro-osseous tissue for the up-side-down grafted group. This result suggests the difference in the character of chondrocytes accoding to the layr. lnthe third experiment, callo-osseous tissue obtained 10 days after iliac osteotomy were implanted to osteo-chondral defects made on patellar groove of raddit's femur, using 88 rabbits. defects where immature callus callus were implanted were healed by hyaline cartilage 3 months postoperativery. These results give us much information, to repair osteochondral defects on joint surface and showed the diffrence of cell character, depending on cartilage cell layr.

本研究的主要目的是研究如何修复受损的关节。为此，我们进行了三种实验。首先，将胶原凝胶培养的同种和同种异体软骨细胞移植到近交系大鼠的骨软骨缺损处。术后12个月观察愈合行为，包括免疫组织化学检测II型胶原。长期观察表明，异体骨移植的愈合与同种异体骨移植一样成功，尽管在12周时同种异体骨的软骨下骨形成延迟。另一方面，单纯植入胶原凝胶或保持开放，用透明胶片修复的对照缺损区均未见。在第二个实验中，为了研究不同细胞层软骨细胞钙化能力的差异，我们进行了自体关节腔原位移植，并将移植物倒置植入。结果表明，原位移植组修复基本完全，而倒置移植组关节表面被纤维骨性组织覆盖。这一结果提示软骨细胞的性质随Layr的不同而不同。实验三：取88只兔，截骨后10天取骨痂组织，植入兔股骨干髌骨沟形成的骨软骨缺损处。术后3个月未成熟骨痂植入处以透明软骨愈合。这些结果为我们修复关节表面的骨软骨缺损提供了大量的信息，并显示了不同软骨细胞层的细胞特性的差异。