Visualization of DNA binding protein in situ by using double strand DNA fragment, and an attempt of inhibition of the transcription factors' DNA binding by using double strand DNA fragment.

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We investigated following projects1 : Visualization of DNA binding protein in situ by using double strand DNA fragment.2 : An attempt for inhibition of transcription factors'DNA binding by using double strand DNA fragment.For the project3 #1, we have succeeded in establishing the method. We used ^<35>S labeled double strand DNA fragment (Ca.20 base par) which has a AP-1 or CRE motif for the detection of binding protein. The positive signal was observed in cell nucleus.For the project #2, we developed a method which allowed the efficient transportation of DNA fragment into cell nucleus. This was achieved by conjugating nuclear transport signal peptide from SV40 Large T antigen and wrapping by liposome.