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Development of a New System for Evaluation of Retinal Microcirculation with the Use of Heat-sensitive Liposomes

开发利用热敏脂质体评估视网膜微循环的新系统

基本信息

批准号：
05557074
负责人：
HONDA Yoshihito
金额：
$ 12.93万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

We have developed the new system for evaluation of retinal microcirculation with heat-sensitive liposomes containing carboxyfluorescein. Carboxyfluorescein was released by heat effect of focal diode laser application. This method allowed three dimensional observation of retinal microcirculation with little choroidal background fluorescence.We have developed a new technique for analysis of leukocyte dynamics in retinal microcirculation. This method consisted of a nuclear dye of acridine orange and scanning laser ophthalmoscopy. We have investigated leukocyte role in various retinal vascular disease models with the use of the method. First, our results suggested that leukocyte accumulation following occlusion of retinal veins may be involved in persistent macula edema that we often observe in clinical cases. Second, interferon alpha increased leukocyte adherence and entrapment in retinal microcirculation. This finding suggested that leukocyte may be related to the occurrence of interferon-induced retinopathy.We evaluated microcirculatory alterations of retinal in a spontaneous model of non-insulin-dependent diabetes with the use of fluorescein dye dilution technique. The results demonstrated that retinal blood flow of the model rats decreased one month after development of diabetes. This finding was recognized 5 months after the initiation of DM.We have reported leukocyte staining with indocyanine green in vivo. We evaluated leukocyte dynamics in choroidal microcirculation in rats.

我们开发了用含有羧基荧光素的热敏脂质体评估视网膜微循环的新系统。通过聚焦二极管激光应用的热效应释放羧基荧光素。这种方法可以在几乎没有脉络膜背景荧光的情况下对视网膜微循环进行三维观察。我们开发了一种分析视网膜微循环中白细胞动态的新技术。该方法由吖啶橙核染料和扫描激光检眼镜组成。我们使用该方法研究了白细胞在各种视网膜血管疾病模型中的作用。首先，我们的结果表明，视网膜静脉阻塞后白细胞积聚可能与我们在临床病例中经常观察到的持续性黄斑水肿有关。其次，干扰素α增加了白细胞在视网膜微循环中的粘附和滞留。这一发现提示白细胞可能与干扰素诱发的视网膜病变的发生有关。我们利用荧光素染料稀释技术评估了自发性非胰岛素依赖型糖尿病模型中视网膜微循环的改变。结果表明，模型大鼠患糖尿病1个月后视网膜血流量减少。这一发现在 DM 开始 5 个月后得到认可。我们报道了体内吲哚菁绿对白细胞的染色。我们评估了大鼠脉络膜微循环中的白细胞动态。