Pathophysiobgical Study of Retinal Ischenia-with Ion-seledive Microelectrode

离子选择性微电极视网膜缺血的病理生理学研究

• 批准号: 03404051
• 负责人: HONDA Yoshihito
• 金额: $ 11.52万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03404051/
• 关键词: Electroretinogram; Ishemia; Potassium ion; Hydrogen ion; Microelectrode; Free radical; SOD; Catalase; ネコ網膜; 色素上皮細胞

We studied the electrophysiological change of the retina during ischemia-reperfusion in vivo cat. The electroretinogram (ERG), intraretinal ERG with microelectrodes and the change of ion concentration in the retina were recorded. In order to observe the change in potentials during reperfusion, retinal ischemia was induced by raising intraocular pressure. The ERG b- and c-wave after 10-30 minutes of ischemia were gradually recovered in 6 hours of reperfusion. After 30 minutes of ischemia complete recovery was not observed. To investigate the role of free radicals in reperfusion injury, superoxide dismutase and catalase, which were free radical scavengers, were given intravenously. Both agents did not show the significant improvement of ERG, although during early period the remarkable recovery of c-wave was observed. The intraretinal recordings showed similar recovery to the ERG.The photoreceptors and retinal pigment epithelium vere also damaged. The fact that the light-evoked alkalinizaton outside photoreceptors was reduced few hous after reperfusion suggests the reduction of photoreceptor's metabolism. The decrease of light-evoked [K+] change in that space suggests that the ion channel or pump function of photoreceptors is reduced.

我们研究了猫体内缺血再灌注过程中视网膜的电生理变化。记录视网膜电图（ERG）、微电极视网膜内ERG以及视网膜内离子浓度的变化。为了观察再灌注过程中电位的变化，通过升高眼压诱发视网膜缺血。缺血10-30分钟后的ERG b波和c波在再灌注6小时内逐渐恢复。缺血30分钟后没有观察到完全恢复。为了研究自由基在再灌注损伤中的作用，静脉注射自由基清除剂超氧化物歧化酶和过氧化氢酶。尽管在早期观察到c波显着恢复，但这两种药物均未显示出ERG的显着改善。视网膜内记录显示与ERG相似的恢复。光感受器和视网膜色素上皮细胞也受损。再灌注后数小时内光感受器外部光引发的碱化减少，这一事实表明光感受器的新陈代谢减少。该空间中光引发的 [K+] 变化的减少表明光感受器的离子通道或泵功能降低。

项目成果

坂上 欧,桐生 純一,竹内 篤,山本 文昭,本田 孔士: "エンドセリンの網膜血管に対する作用。" 日本眼科学会雑誌. 96. (1992)

O Sakagami、Junichi Kiryu、Atsushi Takeuchi、Fumiaki Yamamoto、Koji Honda：“内皮素对视网膜血管的影响。”日本眼科学会杂志 96。（1992 年）

Yoshihito Honda,Fumiaki Yamamoto,Atsushi Takeuch: "Effects ofsodium iodate on〔H^+〕_0 outside rod photoreceptors in cat." Investigative Pphthalmology 8 Visnal Science(Supple.). 32. 837 (1991)

Yoshihito Honda、Fumiaki Yamamoto、Atsushi Takeuch：“碘酸钠对猫外部视杆光感受器的影响。” 调查眼科学 8 Visnal Science（补充）32. 837（1991）。

Hiroi, Kano: "Intraretinal study of cat electvoretinagram during retival is chemia-reperfusicn with K_+-microelectrodes" Investigative Ophthaluology & Uisual Science. (in press). (1994)

Hiroi，Kano：“猫视网膜电图在再生过程中的视网膜内研究是用 K_ 微电极进行化学再灌注”调查眼科

Fumiaki Yamamoto: "Effects of retinal ischemia-reperfusion on pH outside rod photoreceptors in cats." Investigative Ophthalmology & Visual Scieuve(Suppl.). 33. (1992)

Fumiaki Yamamoto：“视网膜缺血再灌注对猫视杆细胞外部 pH 值的影响。”

Yamamoto, Fumiaki: "Effects of isdnevia-reperfusion on light-eucked alkalinization aitside rod photoreceptors in the cat retina" Ophthalmic Research. 25. 371-377 (1993)

Yamamoto，Fumiaki：“isdnevia 再灌注对猫视网膜中光吸碱化辅助杆光感受器的影响”眼科研究。