Development of specific substances evaluated by the regulation of glucose transporter involved in aging of brain

首先，我们试图阐明SAMP8的脑细胞能量代谢特征，SAMP8是加速衰老易感菌株的一个亚株。我们发现，在大脑出现缺陷之前的4-8周龄，D-葡萄糖的氧化速率高于年龄匹配的SAMR1和SAMR2，并且细胞表膜葡萄糖转运蛋白的增加参与了代谢的增加。这两种增加都被限制在4-8周龄和大脑皮层。接下来，我们研究了氧自由基的参与与上述能量代谢变化的关系。我们发现，与SAMR1相比，SAMP8大脑皮层的过氧化脂质和蛋白质羰基含量仅在4-8周龄时出现一过性增加。相应地，SAMP8脑细胞中过氧化氢的含量在4-8周龄时增加。比较两株菌株大脑皮层过氧化物酶的各种酶活性。在SAMP8中观察到过氧化氢酶活性的降低和酰基辅酶A氧化酶活性的升高。这些结果很可能为了解脑内启动衰老记忆损害过程的机制提供了线索，并有助于开发新的衰老治疗物质。

First, we tried to clarify the characteristic of the energy metabolism in the brain cells of SAMP8, a substrain of accelerated senescence-prone strain. We found that the rate of D-glucose oxidation at 4- to 8-weeks of age, just before the appearance of deficits in the brain, was higher than that in age-matched SAMR1 and SAMR2 and that the increase in glucose transporter protein in the cell surface membrane was involved in the increased metabolism. Both the increases were restricted at 4- to 8-weeks of age and the cerebral cortex.Next, we examined the involvement of oxygen free radicals in relation to the above-mentioned change in the cnergy metabolism. We found the transient increase in the contents of lipid peroxides and protein carbonyl only in the cerebral cortex of SAMP8 at 4- to 8-weeks of age in comparison with SAMR1. Accordingly, the content of hydrogen peroxide was increased in the cerebral cells of SAMP8 at 4- to 8-weeks of age. In comparison of the various enzyme activities in the peroxisome in the cerebral cortex between the two strains. The decrease in the catalase activity and increase in acyl-CoA oxidase activity were observed in SAMP8.It is quite likely that these results provide some clues towards understanding the mechanism responsible for initiating the process of senile memory impairment in the brain and contribute the development of new therapeutic substances for aging.