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Development of small caliber vascular graft with stabulizing blood-material interface with monolayr absorbed protein layr.

开发具有单层吸收蛋白层稳定血液-材料界面的小口径血管移植物。

基本信息

批准号：
05558117
负责人：
OKANO Teruo
金额：
$ 7.81万
依托单位：
Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Although many synthetic vascular grafts have been developed and evaluated experimentally or clinically, none of them have met long-term patency when applied as small diameter vascular substitution. We have developed a small caliber vascular graft (3 mm id.) using 2-hydroxyethylmetacrylate (HEMA) and Stirene (St) block copolymer coating. HEMA-St block copolymer formed micro-domain structure and demonstrated improved blood compatibility in both in vitro and in vivo experiments. The graft consists of three layred structure : Dacron for the outer layr, polyurethane in middle layr, and a HEMA-St block copolymer coating for the inner layr. The grafts were evaluated in dogs bilateral carotid replacement model. All grafts were patent without detachable thrombi along the graft length including anastomotic sites. Scanning electron micrographs of retrieved graft lumen showed fairly clean surfaces covered with a homogeneous protein-like layr without microthrombi or endotherial ell lining.The thickness of the surface protein layr measured by a transmission electron microscopy was about 200A which can be described as monolayr protein absorption regardless of implantation period of as much as one year. A stable monolayr absorbed protein layr formed on HEMA-St block copolymer surfaces demonstrated nonthorombogenic activities in vivo and secure long-term patency of small caliber vascular grafts with the absence of an endotherial cell lining.

尽管已经开发了许多人造血管移植物并进行了实验或临床评价，但当用作小直径血管替代物时，它们中没有一个满足长期通畅性。我们已经开发了一种小口径血管移植物（内径3 mm）。使用甲基丙烯酸2-羟乙酯（HEMA）和苯乙烯（St）嵌段共聚物涂层。HEMA-St嵌段共聚物形成微区结构，并在体外和体内实验中显示出改善的血液相容性。该人工血管由三层结构组成：外层为涤纶，中间为聚氨酯，内层为HEMA-St嵌段共聚物涂层。在犬双侧颈动脉置换模型上对移植物进行评价。所有移植物均通畅，沿移植物长度（包括吻合部位）沿着无可解脱血栓。移植物管腔的扫描电镜显示表面相当干净，表面覆盖均匀的蛋白质样层，无微血栓或内皮细胞衬里，透射电镜测量表面蛋白质层厚度约为200埃，可描述为单层蛋白质吸收，与长达一年的植入期无关。在HEMA-St嵌段共聚物表面形成的稳定单层吸收蛋白层在体内表现出非致血栓活性，并在缺乏内皮细胞衬里的情况下确保小口径血管移植物的长期通畅性。