Functionalization of thermoresponsive nano-interfaces and thermally regulated detachment behaviors of adherent proteins and cells

热响应纳米界面的功能化以及粘附蛋白质和细胞的热调节分离行为

• 批准号: 20300169
• 负责人: OKANO Teruo
• 金额: $ 12.06万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20300169/
• 关键词: インテリジェント材料; 表面機能化; 温度応答性; 細胞シート; ナノ表面; 精密重合; poly(N-isopropylacrylamide); 温度応答性表面; 細胞シート工学; RAFT重合; 高分子ブラシ; リビングラジカル重合

In this study, surface-initiated RAFT polymerization was demonstrated to materialize new type thermoresponsive PIPAAm brush surfaces for cell sheet harvest. The molecular weight and graft density of grafted PIPAAm chains was controllable through this grafting method. Therefore, optimal thermoresponsive surfaces were able to be designed for cell sheet preparation. Furthermore, RAFT-mediated block copolymerization achieved the stripe-like micropatterning of thermoresponsive PIPAAm domains and cell-repellent polymer domains. Human fibroblasts were aligned on the micropatterned polymer brush surfaces and the aligned cells were harvested as a cell sheet with well-controlled orientational structures.

在这项研究中，表面引发的RAFT聚合被证明可以实现新型热敏PIPAAm刷状表面，用于细胞片收获。通过这种接枝方法可以控制接枝PIPAAm链的分子量和接枝密度。因此，可以设计最佳的热响应表面用于细胞片的制备。此外，raft介导的嵌段共聚实现了热响应PIPAAm结构域和细胞排斥聚合物结构域的条纹状微图案。人类成纤维细胞排列在微图案聚合物刷表面，排列后的细胞被收获为具有良好定向结构的细胞片。

项目成果

Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

• DOI: 10.1016/j.chroma.2010.07.067
• 发表时间: 2010-09-17
• 期刊: JOURNAL OF CHROMATOGRAPHY A
• 影响因子: 4.1
• 作者: Mizutani, Aya;Nagase, Kenichi;Okano, Teruo
• 通讯作者: Okano, Teruo

Thermo-Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization

• DOI: 10.1002/mabi.201000312
• 发表时间: 2011-03-10
• 期刊: MACROMOLECULAR BIOSCIENCE
• 影响因子: 4.6
• 作者: Nagase, Kenichi;Watanabe, Minami;Okano, Teruo
• 通讯作者: Okano, Teruo

Temperature Responsive Polymeric Surfaces for Biomedical Applications

用于生物医学应用的温度响应聚合物表面

• 发表时间: 2009
• 作者: Kawai T;et al.;江渝;橋本謙;岡野光夫
• 通讯作者: 岡野光夫

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Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography

• DOI: 10.1016/j.chroma.2009.11.073
• 发表时间: 2010-01-22
• 期刊: JOURNAL OF CHROMATOGRAPHY A
• 影响因子: 4.1
• 作者: Mizutani, Aya;Nagase, Kenichi;Okano, Teruo
• 通讯作者: Okano, Teruo