Functionalization of thermoresponsive nano-interfaces and thermally regulated detachment behaviors of adherent proteins and cells

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In this study, surface-initiated RAFT polymerization was demonstrated to materialize new type thermoresponsive PIPAAm brush surfaces for cell sheet harvest. The molecular weight and graft density of grafted PIPAAm chains was controllable through this grafting method. Therefore, optimal thermoresponsive surfaces were able to be designed for cell sheet preparation. Furthermore, RAFT-mediated block copolymerization achieved the stripe-like micropatterning of thermoresponsive PIPAAm domains and cell-repellent polymer domains. Human fibroblasts were aligned on the micropatterned polymer brush surfaces and the aligned cells were harvested as a cell sheet with well-controlled orientational structures.