Development of temperature-responsive culture dishes which allow viable cell sheet recovery
开发可回收活细胞片的温度响应型培养皿
基本信息
- 批准号:08558101
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Co-culture of multiple cell types, a critical pre-requisite for in vitro tissue construction, often results in domination of co-culture cell populations by one cell type. Natural tissues are often observed to comprise multiple cell types forming distinct layers in stratified architectures. Several cell types are co-organized into defined cell layers in various organs and tissues where stratification of cell layers is critical to cell-cell interaction and, at a larger level, to organ function. We have challenged a novel strategy to reconstruct tissue architectures from cell sheets. A unique temperature-responsive culture surface permits harvest of cultured various cell types as confluent, intact cell monolayer sheets without typical destructive enzymatic treatment. Overlaying two monolayer sheets of hepatocytes and endothelial cells provides a viable construct for in vitro fabricated liver lobule-like tissue. Cultured lamellar cell sheets preserve each cell phenotype and basic cell functions. This general strategy has utility in studies of cell-cell interactions, tissue neogenesis and the design of many types of tissue-engineered biomaterials.
多种细胞类型的共培养是体外组织构建的关键前提,通常会导致一种细胞类型主导共培养细胞群体。自然组织经常被观察到由多种细胞类型组成,在层状结构中形成不同的层。在不同的器官和组织中,几种细胞类型被共同组织成确定的细胞层,其中细胞层的分层对于细胞与细胞的相互作用以及在更大的水平上对器官的功能至关重要。我们挑战了一种从细胞片重建组织结构的新策略。独特的温度响应型培养表面允许将培养的各种类型的细胞收获为融合的、完整的细胞单层片,而无需典型的破坏性酶处理。覆盖两层单层肝细胞和内皮细胞为体外构建肝小叶样组织提供了一种可行的结构。培养的板层细胞片保留了每个细胞的表型和基本细胞功能。这一通用策略在细胞-细胞相互作用、组织新生和许多类型的组织工程生物材料的设计中具有实用价值。
项目成果
期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A.Kikuchi, T.Okano: "Temperature-Responsive Polymers as On-Off Switches for Intelligent Biointerfaces" Biorelated Polymers and Gels (T.Okano ed.). Academic Press. 1-28 (1998)
A.Kikuchi、T.Okano:“温度响应聚合物作为智能生物界面的开关”生物相关聚合物和凝胶(T.Okano 编辑)。
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A.Kikuchi, M.Okuhara, F.Karikusa, Y.Sakurai, T.Okano: "Tow-dimensional mannipulation of confluently cultured vascular endothelial cells using temperature-responsive poly (N-isopropylacrylamide)-grafted surfces" Journal of Biomaterial Sciences, Polymer Edi
A.Kikuchi、M.Okuhara、F.Karikusa、Y.Sakurai、T.Okano:“使用温度响应型聚(N-异丙基丙烯酰胺)接枝表面对汇合培养的血管内皮细胞进行二维操纵”生物材料科学杂志,
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H.von Recum: "Novel thermally reversible hydrogel for detachable cell culture substrate" J.Biomed.Mater.Res.(in press). (1998)
H.von Recum:“用于可分离细胞培养基质的新型热可逆水凝胶”J.Biomed.Mater.Res.(正在印刷中)。
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- 影响因子:0
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Y.Kaneko, S.Nakamura, K.Sakai, A.Kikchi, T.Aoyagi, Y.Sakurai, T.Okano: "Deswelling mechanism for com-type grated Poly (N-isopropylacrylamide) Hydrogels woth rapid temperature responses" Polymer Gels and Networks. (in press).
Y.Kaneko、S.Nakamura、K.Sakai、A.Kikchi、T.Aoyagi、Y.Sakurai、T.Okano:“具有快速温度响应的 com 型磨碎聚(N-异丙基丙烯酰胺)水凝胶的消溶胀机制” 聚合物凝胶
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- 影响因子:0
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T.Yakushiji, K.Sakai, A.Kikuchi, T.Aoyagi, Y.Sakurai, T.Okano: "Design of Thermo-responsive surfaces for Temperature-regulated hydrophobic chromatography and separation of steroids" Kagakukougaku Ronbunnshu. 24. 205-210 (1998)
T.Yakushiji、K.Sakai、A.Kikuchi、T.Aoyagi、Y.Sakurai、T.Okano:“用于温度调节疏水色谱和类固醇分离的热响应表面的设计” Kagakukougaku Ronbunnshu。
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OKANO Teruo其他文献
Ex Vivo Gene Delivery for Fabrication of Hepatocyte Sheet Tissues Secreting Angiogenic Factors
用于制造分泌血管生成因子的肝细胞片组织的离体基因传递
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
KOBAYASHI Jun;LEE Hyukjin;YAMATO Masayuki;OKANO Teruo - 通讯作者:
OKANO Teruo
Psychoacoustic Masking Effect for Robust Speech Communication Robot
鲁棒语音通信机器人的心理声学掩蔽效应
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
KOBAYASHI Jun;ARISAKA Yoshinori;YUI Nobuhiko;YAMATO Masayuki;OKANO Teruo;計 宇生;Yoshikazu Miyanaga - 通讯作者:
Yoshikazu Miyanaga
Gene delivery for creation of hepatocyte sheets secreting angiogenic factors
用于创建分泌血管生成因子的肝细胞片的基因递送
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
KOBAYASHI Jun;LEE Hyukjin;YAMATO Masayuki;OKANO Teruo - 通讯作者:
OKANO Teruo
Psychoacoustic Masking Effect for Noise Robust Speech Recognition Robot
噪声鲁棒语音识别机器人的心理声学掩蔽效应
- DOI:
- 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
KOBAYASHI Jun;LEE Hyukjin;YAMATO Masayuki;OKANO Teruo;Yoshikazu Miyanaga - 通讯作者:
Yoshikazu Miyanaga
Creation of transplantable hepatocyte sheet tissues by using thermoresponsive cell culture surfaces
使用热响应细胞培养表面创建可移植的肝细胞片层组织
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
KOBAYASHI Jun;LEE Hyukjin;YAMATO Masayuki;OKANO Teruo - 通讯作者:
OKANO Teruo
OKANO Teruo的其他文献
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{{ truncateString('OKANO Teruo', 18)}}的其他基金
Functionalization of thermoresponsive nano-interfaces and thermally regulated detachment behaviors of adherent proteins and cells
热响应纳米界面的功能化以及粘附蛋白质和细胞的热调节分离行为
- 批准号:
20300169 - 财政年份:2008
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of novel intelligent tissue culture surfaces to accelerate cell proliferation and recover cell sheet under serum free conditions, using physiologically active peptides
使用生理活性肽开发新型智能组织培养表面,以加速细胞增殖并在无血清条件下恢复细胞片层
- 批准号:
16200036 - 财政年份:2004
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
A Novel Transplantable Epidermal Cell Sheet Noninvasively Harvested from Thermoresponsive Cell Culture Dishes without Proteolytic Enzymes
从不含蛋白水解酶的热响应细胞培养皿中非侵入性收获新型可移植表皮细胞片
- 批准号:
13557148 - 财政年份:2001
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of novel co-culture system of parenchymal cells and nonparenchymal cells on thermoresponsive polymer-grafted dish surfaces
热响应聚合物接枝培养皿表面实质细胞和非实质细胞新型共培养系统的开发
- 批准号:
13308055 - 财政年份:2001
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular design of graft-type poly (N-isopropylacrylamide) gels containing rapid thermo-response
快速热响应接枝型聚(N-异丙基丙烯酰胺)凝胶的分子设计
- 批准号:
08455458 - 财政年份:1996
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of small caliber vascular graft with stabulizing blood-material interface with monolayr absorbed protein layr.
开发具有单层吸收蛋白层稳定血液-材料界面的小口径血管移植物。
- 批准号:
05558117 - 财政年份:1993
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Temperature-modulated surfaces for recovery of cultured cells
用于回收培养细胞的温度调节表面
- 批准号:
04453108 - 财政年份:1992
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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