Joint Study of Immunomodulation for Corneal Graft Survival

角膜移植物存活免疫调节联合研究

• 批准号: 05045051
• 负责人: ISHIKAWA Satoshi
• 金额: $ 3.84万
• 依托单位: School of Medicine, Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05045051/
• 关键词: Corneal grafting; Pig; Inbred rat; Ultraviolet; Monochromator; 紫外線; ブタ; 大動物実験

We have used Monochromator (CT-25 IV,Jasco) for corneal graft surviving and choose specific narrow band for irradiation with the support of Mr.Keith Langmack in Medical Physics, Addenbrooke's Hospital, Cambridge. Dr.Atsushi Minamoto, Dept.of Ophthalmology, Hiroshima University who was studying Transplantation Immunology under the supervision of Mr.Watson helped for corneal grafting and post-operative e observation. We have finally detected the specific band and the minimum dose for corneal graft surviving (300nm, 200mJ/cm^2).Dr.Katami had visited to Cambridge in November to start the pig corneal grafting using the Monochromator. However, in the same condition, the effect of ultraviolet to the pig cornea was not so significant than that the rat for long-term corneal graft surviving. Dr.William B.Amos, Molecular Biology, MRC,Cambridge helped us to investigate inability of ultraviolet effect in pig corneal grafting. With the powerful instrument of confocal microscope which can show three … More dimensional microscopic image of the object, we understood that pig endothelial cells were damaged so that the graft itself could not support for maintain ace of the cornea. This indicates that dose of ultraviolet to the pig cornea should be lower that that of rat cornea for graft survival. We have summarized the experiments as follows.1.Inbred rat work--- This is quite useful model for detect specific wave length and total dose of ultraviolet for corneal graft surviving.2.Pig work---The work needs more efforts to detect safety dose of ultraviolet for corneal graft surviving. Confocal microscopic examination is necessarily for confirmation of safety level of ultraviolet.3.Clinical trial--- The method we used in pig would be useful when we would try to utilize ultraviolet for clinical corneal transplantation. Mr.Malcolm Kurr-Muir, cornea specialist is responsible for pilot study in Dept.of Ophthalmology, University of Cambridge. After the pilot study confirmed without side effect, we will expand the clinical trial in different institutes in the world. Less

在剑桥Addenbrooke医院医学物理学基思朗马克先生的支持下，我们使用单色仪（CT-25 IV，Jasco）进行角膜移植物存活，并选择特定的窄带进行照射。广岛大学眼科学系的Atsushi Minamoto博士在沃森先生的指导下学习移植免疫学，帮助进行角膜移植和术后观察。我们最终检测到了特异性谱带和角膜移植物存活的最小剂量（300 nm，200 mJ/cm ^2）。Katami博士于11月访问了剑桥，开始使用单色仪进行猪角膜移植。但在相同条件下，紫外线对猪角膜移植片长期存活的影响不如对大鼠角膜移植片长期存活的影响显著。William B.Amos博士，分子生物学，MRC，剑桥帮助我们研究了猪角膜移植中紫外线效应的失效。借助强大的共聚焦显微镜， ...更多信息 通过对该物体的三维显微图像的观察，我们了解到猪内皮细胞受损，使得移植物本身不能支持维持角膜的ACE。这表明，猪角膜的紫外线照射剂量应低于大鼠角膜，以保证移植物的存活。我们将实验总结如下。1.近交系大鼠工作-这是检测角膜移植物存活的紫外线特定波长和总剂量的非常有用的模型。2.猪工作-这项工作需要更多的努力来检测角膜移植物存活的紫外线安全剂量。共聚焦显微镜检查是确定紫外线安全水平的必要条件。3.临床试验-我们在猪身上使用的方法将是有用的，当我们试图利用紫外线进行临床角膜移植时。Malcolm Kurr-Muir先生，角膜专家，负责剑桥大学眼科学系的初步研究。在初步研究证实没有副作用后，我们将在世界各地的不同机构扩大临床试验。少

项目成果

M.Katami,P.W.Madden,D.J.G.White,P.G.Watson: "The extent of immunological privilege of orthotopic corneal grafts in the inbred rat." Transplantation.48. 371-376 (1989)

M.Katami、P.W.Madden、D.J.G.White、P.G.Watson：“近交系大鼠原位角膜移植物的免疫特权程度。”

M.Katami.: "Is a corneal transplantation immunologically privileged from graft rejection?" Annals Academy of Medicine Singapore.20. 432-438 (1991)

M.Katami.：“角膜移植在免疫学上是否能免受移植物排斥？”

M.Katami,D.J.G.White and P.G.Watson.: "Site-specific immunosuppression for corneal graft survival in the rat." Transplant.Proc.24. 1406-1408 (1992)

M.Katami、D.J.G.White 和 P.G.Watson.：“位点特异性免疫抑制可促进大鼠角膜移植物的存活。”