Difference in the properties of contraction in vassular and intestinal smooth mscles

血管和肠道平滑肌收缩特性的差异

• 批准号: 05404015
• 负责人: KARAKI Hideaki
• 金额: $ 21.44万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05404015/
• 关键词: Smooth Muscle; Phasic Muscle; Tonic Muscle; Cytosolic Ca level; Platelet activating factor; Endothelin; phasic筋; tonic筋; 収縮; 細胞内カルシウム; PAF; カルシウム; 収縮タンパク質; Caチャネル

In some smooth muscles, stimulation by elevated external K^+ concentration or receptor agonists increases the amplitude of phasic contraction, with little, if any, elevation in tone. These muscles have been classified as "phasic" type. This type contrasts with "tonic" type which maintain force generation over time without visible rhythmic fluctuations. Mechanism responsible for these differences has not been examined except to speculate that differences in membrane electrical activities may be responsible for. The major emphasis of this study was to characterize the physiological and pharmacological properties between these smooth muscle subtypes. In this study, we have revealed 1) role of PAF in phasic muscle, 2) subtypes of endothelin receptors in phasic and tonic muscles, 3) subtypes of purinoceptors in tonic muscle, 4) role of protein kinase C in phasic and tonic muscle, etc.

在一些平滑肌肉中，外源性K~(++)浓度升高或受体激动剂的刺激增加了时相收缩的幅度，但音调几乎没有升高。这些肌肉被归类为“相位型”肌肉。这种类型与“主音”类型不同，“主音”类型会随着时间的推移保持力量的产生，而不会出现明显的节奏波动。除了推测膜电活动的差异可能是造成这些差异的原因外，还没有研究这些差异的机制。这项研究的主要重点是表征这些平滑肌亚型之间的生理和药理特性。在本研究中，我们揭示了PAF在时相肌中的作用，2)时相肌和紧张肌中内皮素受体的亚型，3)紧张肌中的嘌呤受体亚型，4)蛋白激酶C在时相肌和紧张肌中的作用等。

项目成果

KITAJIMA,S.,OZAKI,H.& KARAKI,H.: "The effects of ATP and αβ-methylene-ATP on cytosolic Ca^<2+> level and force in isolated rat aorta." Br.J.Pharmacol.110. 263-268 (1993)

KITAJIMA,S.,OZAKI,H.& KARAKI,H.：“ATP 和 αβ-亚甲基-ATP 对离体大鼠主动脉中胞质 Ca^2+ 水平和力的影响 Br.J.Pharmacol.110” 263-268（1993）

TAKIZAWA,S.,HORI,M.,OZAKI,H.& KARAKI,H.: "Effects of isoquinoline derivatives,HA1077 and H-7,on cytosolic Ca^<2+> level and contraction in vascular smooth muscle." Eur.J.Pharmacol.250. 431-437 (1993)

泷泽S.、堀M.、尾崎H.

Ozaki,H: "Different Ca^<2+> sensitivity in phasic and tonic types of smooth muscles" Biol. Signals. 2. 253-262 (1993)

Ozaki,H：“平滑肌的阶段性和强直性类型中不同的 Ca^<2> 敏感性”Biol。

HOSOYA,N.,MITSUI,M.,YAZAMA,F.,ISHIHARA,H.,KARAKI,H.,HARTSHORNE,D.J.& MOHRI,H.: "Changes of cytoskeletal structures in cultured smooth muscle cells induced by calyculin-A." J.Cell.Sci.105. 883-890 (1993)

细谷，N.，三井，M.，矢座，F.，石原，H.，卡拉基，H.，哈茨霍恩，D.J.

KWON,S.C.,OZAKI,H.,HORI,M.& KARAKI,H.: "Isoproterenol changes the relationship between cytosolic Ca^<2+> and contraction in guinea pig taenia caecum." Jpn.J.Pharmacol.61. 57-64 (1993)

权S.C.、尾崎H.、堀M.