Terpenoid biosynthesis in the cultivated mushroom Cyclocybe aegerita
栽培蘑菇中萜类化合物的生物合成
基本信息
- 批准号:525829672
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:
- 资助国家:德国
- 起止时间:
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Terpenoids constitute a structurally diverse group of natural products with indispensable applications in the pharmaceutical, nutritional, flavour and fragrance industries. Fungi of the phylum Basidiomycota are known to produce a large variety of terpenoids, yet their biosynthesis pathways remain largely unexplored. The genome of the mushroom model system Cyclocybe aegerita (syn. Agrocybe aegerita) contains nine functional sesquiterpene synthases (STS), of which four are part of biosynthetic gene clusters. By the heterologous expression of these clusters in the related model agaric fungus Coprinopsis cinerea, the peculiar characteristics of these biosynthesis pathways will be investigated in detail. 1) The recently detected bissesquiterpenoids bovistol A-C are assembled by a Diels-Alderase, whose substrate spectrum will be determined by in vitro reactions. 2) Their illudin-precursors contain a three-membered ring structure, which is responsible for their strong cytotoxic effects. Despite the very broad distribution of this skeletal in fungi, the key ring contraction reaction catalysed apparently by P450-type monooxygenases remains an enigma. 3) A new viridiflorene-based diterpene is produced by C. aegerita, which is expected to be produced by the characterised STS Agr2. We will investigate whether this enzyme accepts geranylgeranyl pyrophosphate in addition to farnesyl pyrophosphate. Since we determine the structures and bioactivities of both volatile and non-volatile secondary metabolite products, we will not only further tap the secondary metabolome of the edible mushroom C. aegerita, but also seek to understand the terpenoid biosynthesis of a typical Basidiomycota in general.
萜类化合物是一类结构多样的天然产物,在制药、营养、香料和香料工业中具有不可或缺的应用。担子菌门的真菌已知能产生多种萜类化合物,但它们的生物合成途径在很大程度上仍未被探索。报道了蘑菇模式系统青霉Cyclocybe aegerita(syn.茶树菇(Agrocybe Aegerita)含有9个功能性倍半萜合成酶(STS),其中4个属于生物合成基因簇。通过这些簇在相关模式木耳中的异源表达,将详细研究这些生物合成途径的特殊特征。1)新近检测到的双倍半萜类化合物Bovistol A-C由Diels-Alderase组装而成,其底物光谱将通过体外反应确定。2)它们的伊利定前体含有三元环结构,这是它们具有很强的细胞毒作用的原因。尽管这种骨架在真菌中的分布非常广泛,但显然由P450型单加氧酶催化的关键环收缩反应仍然是一个谜。3)一种新的病毒型二花烯二萜类化合物的产生,该二萜类化合物有望由具有代表性的STS Agr2菌株产生。我们将调查这种酶除了接受法尼基焦磷酸外,是否还接受香叶基香叶基焦磷酸。由于我们确定了挥发性和非挥发性次生代谢物的结构和生物活性,我们不仅将进一步挖掘食用菌青霉的次生代谢组,而且还将试图从总体上了解典型担子菌的萜类生物合成。
项目成果
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Professor Dr. Martin Rühl其他文献
Professor Dr. Martin Rühl的其他文献
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{{ truncateString('Professor Dr. Martin Rühl', 18)}}的其他基金
Volatilome and transcriptome of the mushroom Agrocybe aegerita
蘑菇挥发物组和转录组
- 批准号:
333830069 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Research Grants
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