Cloning of CBP and its regulation mechanism

我们研究了转录因子CREB磷酸化依赖的激活机制（Hagiwara et al ., Cell 1992, Hagiwara et al ., Mol Cell Biol 1993, Shimomura et al ., J Biol Chem 1996）。在本研究中，我们通过改进的低角度旋转阴影方法成功地可视化了CREB/DNA复合物，CREB磷酸化依赖的结构变化以及CREB/CBP复合物的形成。本文中描述的电子显微镜分子间成像应该能够获得有关转录机制的结构信息，这应该补充x射线晶体学获得的证据。

We have investigated phosphorylation-dependent activation mechanism of the transcription factor, CREB (Hagiwara et al, Cell 1992, Hagiwara et al, Mol Cell Biol 1993, Shimomura et al, J Biol Chem 1996). In the present study, we succeeded to visualize the CREB/DNA complexes, phosphorylation-dependent structural changes of CREB,and complex formation of CREB/CBP by an improved low angle rotary shadowing methods. Intermolecular imaging by electron microscopy described in this manuscript should enable structural information to be obtained regarding transcriptional machinery, which should complement evidence gained from X-ray crystallography.