Dynamic Structure of Biosupramole cular System.

本项目旨在利用多相电子自旋共振技术对生物超分子体系进行细致而准确的研究。我们进一步尝试了超分子结构的分析，使用ESR成像方法。在这个项目的第二年，我们专注于两个研究项目，即，生物超体系的动力学结构、生物氧测定以及生物自由基动力学等方面的研究。多种ESR光谱的组合，即，CW X波段、矢量、脉冲多维ESR、CW-ESR成像和脉冲-ESR成像。应用ESR波谱在各种频率水平从1到250 GHz的对齐和厚的多层膜等系统的部分水合磷脂双层，我们几乎确定了分子的有序性，流动性，链倾斜，头基协同性，以及这些属性如何在各种相变的变化。我们进一步应用 关于我们 矢量ESR方法，深入研究蛋白质与脂质的相互作用，包括蛋白质对双层结构的“硬化效应“和“无序效应”。随着250 GHz（9特斯拉）远红外ESR光谱仪的完成，我们将ESR研究扩展到高频和高场区域。从高场光谱分辨率的增加，使我们能够准确地测量磁张量，准确地确定取向的潜力和订购张量和更准确地研究脂质蛋白质interaction.Comparative研究的自旋弛豫在很宽的频率范围（250 GHz）导致更详细的信息的动态结构的生物膜。特别是250 GHz的超高场ESR是复杂生物膜系统动力学研究的里程碑。其次，我们的目标是生物氧测定以及生物自由基动力学。传统的ESR检测很少有生物自由基具有丰富的浓度和适当的弛豫时间。我们应用开发新技术的电子自旋弛豫实验的生物自由基和生物氧，这是为了显着提高光谱分辨率。其中包括脉冲L波段电子自旋回波技术。我们广泛地利用了这项新技术。此外，在体内ESR仪器被用来描绘生物自由基的结构特征。随着我们用于小动物实验的新ESR共振器的完成，我们将把我们的ESR研究扩展到体内。少

The present project is aimed at careful and accurate studies of the biosupra-molecular system using the multi-phase electron spin resonance spectroscopies. We further have attempted an analysis of supra-molecular structure using the ESR imaging method. For the second year of this project, we focused on the two research project, i.e., dynamic structure of biosupra-systems, and biooxymetry as well as dynamics of bioradicals.Firstly, we performed the comprehensive study on the supra-molecular structure via. combination of multiple-ESR spectroscopies i.e., CW X-band, vector, pulse multi-dimensional ESR,CW-ESR imaging and pulsed-ESR imaging. Application of ESR spectroscopies at various frequency level from 1 to 250 GHz on the well-aligned and thick multilayrs of such systems as partially hydrated phospholipid bilayrs, we virtually determined molecular ordering, fluidity, chain tilt, headgroup cooperativity, and how these properties change at the various phase transitions. We further applied … More vector ESR method to a system consisted of lipid-protein complex, and to study in-depth the protein-lipid interaction, including the "hardening-effect"vs. the "disordering-effect" of the protein on the bilayr structure. We extended our ESR studies into the high-frequency and high-field regime with the completion of 250 GHz (9 Tesla) far-infrared ESR spectrometer. The increased spectral resolution from the high fields enabled us to make accurate measurements of magnetic tensors, to accurately determine orienting potentials and ordering tensors and to more accurately study the lipid-protein interaction.Comparative studies of spin-relaxation over a wide range of frequencies(250 GHz)lead to more detailed information on the dynamic structure of biomembranes. In particular, the ultra-high field ESR at 250 GHz is the land mark attainment for such the dynamic study in complex biomembranes systems.Secondly, we aimed at biooxymetry as well as dynamics of bioradicals. The conventional ESR sorely detects few bioladicals having a rich concentration and appropriate relaxation times. We applied developing new techniques for electron-spin relaxation experiments on bioradicals and biooxgens, which are designed to significantly improve spectral resolution. These include a pulsed L-band electron-spin-echo technique. We extensively exploited this new technique. Also, in vivo ESR instrument was used to delineate structural features of bioradicals. We shall be extending our ESR studies into in vivo regime with the completion of our new ESR resonator for small animal experiments. Less