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Dynamic Structure of Biosupramole cular System.

生物超分子系统的动态结构。

基本信息

批准号：
06044236
负责人：
SHIMOYAMA Yuhei
金额：
$ 6.78万
依托单位：
HOKKAIDO UNIVERSITY OF EDUCATION (1995)Okazaki National Research Institutes (1994)
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044236/
关键词：
Bioradicals Biooxymetry Pulse-ESR High Field ESR Multi-Quantum ESR ESR Imaging Spin Labeling Vector ESR 脂質単分子膜空間次元核酸の動的構造パルスESR法 in-vivoESR 生体内フリーラジカル ESRオキシメトリー L-バンドESR

项目摘要

The present project is aimed at careful and accurate studies of the biosupra-molecular system using the multi-phase electron spin resonance spectroscopies. We further have attempted an analysis of supra-molecular structure using the ESR imaging method. For the second year of this project, we focused on the two research project, i.e., dynamic structure of biosupra-systems, and biooxymetry as well as dynamics of bioradicals.Firstly, we performed the comprehensive study on the supra-molecular structure via. combination of multiple-ESR spectroscopies i.e., CW X-band, vector, pulse multi-dimensional ESR,CW-ESR imaging and pulsed-ESR imaging. Application of ESR spectroscopies at various frequency level from 1 to 250 GHz on the well-aligned and thick multilayrs of such systems as partially hydrated phospholipid bilayrs, we virtually determined molecular ordering, fluidity, chain tilt, headgroup cooperativity, and how these properties change at the various phase transitions. We further applied … More vector ESR method to a system consisted of lipid-protein complex, and to study in-depth the protein-lipid interaction, including the "hardening-effect"vs. the "disordering-effect" of the protein on the bilayr structure. We extended our ESR studies into the high-frequency and high-field regime with the completion of 250 GHz (9 Tesla) far-infrared ESR spectrometer. The increased spectral resolution from the high fields enabled us to make accurate measurements of magnetic tensors, to accurately determine orienting potentials and ordering tensors and to more accurately study the lipid-protein interaction.Comparative studies of spin-relaxation over a wide range of frequencies(250 GHz)lead to more detailed information on the dynamic structure of biomembranes. In particular, the ultra-high field ESR at 250 GHz is the land mark attainment for such the dynamic study in complex biomembranes systems.Secondly, we aimed at biooxymetry as well as dynamics of bioradicals. The conventional ESR sorely detects few bioladicals having a rich concentration and appropriate relaxation times. We applied developing new techniques for electron-spin relaxation experiments on bioradicals and biooxgens, which are designed to significantly improve spectral resolution. These include a pulsed L-band electron-spin-echo technique. We extensively exploited this new technique. Also, in vivo ESR instrument was used to delineate structural features of bioradicals. We shall be extending our ESR studies into in vivo regime with the completion of our new ESR resonator for small animal experiments. Less

本项目旨在利用多相电子自旋共振光谱对生物超分子体系进行细致而准确的研究。我们进一步尝试使用ESR成像方法分析超分子结构。在这个项目的第二年，我们专注于两个研究项目，即生物超系统的动态结构和生物氧学以及生物自由基的动力学。首先，我们对其超分子结构进行了全面的研究。多重ESR光谱的组合，即连续波x波段、矢量、脉冲多维ESR、CW-ESR成像和脉冲ESR成像。利用1 ~ 250 GHz不同频率的ESR光谱对部分水合磷脂双分子层等排列良好的多层体系进行研究，我们确定了分子的有序度、流动性、链倾斜、头基协同性以及这些性质在不同相变下的变化。我们进一步将更多的载体ESR方法应用于一个由脂质-蛋白复合物组成的系统，并深入研究了蛋白-脂质的相互作用，包括“硬化效应”与“硬化效应”。蛋白质对双层结构的“无序效应”。随着250 GHz（9特斯拉）远红外ESR光谱仪的建成，我们将ESR研究扩展到高频和高场区。高场提高的光谱分辨率使我们能够精确测量磁张量，准确确定定向电位和有序张量，并更准确地研究脂质-蛋白质相互作用。在宽频率范围内（250 GHz）对自旋弛豫进行比较研究，可以获得有关生物膜动态结构的更详细信息。其中，250 GHz的超高场ESR是复杂生物膜系统动态研究的里程碑式成就。其次，我们的目标是生物氧合学以及生物自由基的动力学。传统的ESR只能检测到少量具有丰富浓度和适当松弛时间的生物自由基。我们在生物自由基和生物氧根的电子自旋弛豫实验中应用了新技术，旨在显著提高光谱分辨率。其中包括脉冲l波段电子自旋回波技术。我们广泛地利用了这项新技术。此外，还利用体内ESR仪器描绘了生物自由基的结构特征。随着小型动物实验的新ESR谐振器的完成，我们将把ESR研究扩展到体内制度。少