Development of Multi-frequency Vector EPR Spectrometer by White-noise Field Modulation Method.

白噪声场调制法多频矢量EPR光谱仪的研制。

• 批准号: 07555001
• 负责人: SHIMOYAMA Yuhei
• 金额: $ 4.61万
• 依托单位: Hokkaido University of Education
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07555001/
• 关键词: Vector ESR; selective detection; continuous wave; phase spectra; amplitude spectra; biomembrane; saturation transfer; field modulation

Development and applications of Vector ESR spectroscopies were reviewed. Vector ESR was developed on the basis of saturation transfer ESR method. We applied the method on the various biomaterial systems, and found that Vector ESR gives a universal method for selective detection in the continuous wave regime. We will further indicate the potentiality vector ESR to the study of biomolecular dynamics.Thus far, biological applications of vector ESR spectroscopy are just the beginning, and mainly focused on the two area, namely protein and biomembrane systems. Vistnes studied dynamics of spin-labeled hemoglobin using the phase signal of ST-ESR detection. Watari et al. Showed the feasibility of vector ESR spectroscopy as applied on the flavin enzyme system, and found the sensitivity of the phase spectrum. They also indicated that both the amplitude and phase spectra can detected the unknown high spin signal from ferrihemoglobin system.

综述了矢量ESR谱的发展和应用。矢量ESR法是在饱和转移ESR法的基础上发展起来的。我们将该方法应用于各种生物材料系统，并发现矢量ESR给出了一个通用的方法，在连续波制度的选择性检测。到目前为止，矢量ESR波谱在生物学中的应用还处于起步阶段，主要集中在蛋白质和生物膜两个方面。Vistnes利用ST-ESR检测的相位信号研究了自旋标记血红蛋白的动力学。瓦塔里等人展示了矢量ESR光谱应用于黄素酶体系的可行性，并发现了相位谱的灵敏度。结果还表明，振幅谱和相位谱都能检测到高铁血红蛋白体系中未知的高自旋信号。

项目成果

T.Komatsu, T.Takamura and Y.Shimoyama: "Fabrication, Spatial and Spin Chain Structure of Langmuir-Blodgett Films of Copper tetra-t-butyl Phtalocyanine" Modern Applications of ESR. 1. 20-24 (1997)

T.Komatsu、T.Takamura 和 Y.Shimoyama：“四叔丁基酞菁铜的 Langmuir-Blodgett 薄膜的制造、空间和自旋链结构”ESR 的现代应用。

下山雄平: "ベクトルESR(第7章)" 共立出版社・新高分子実験学講座・第五巻・高分子の構造, 29 (1995)

Yuhei Shimoyama：“矢量ESR（第7章）”共立出版，新聚合物实验课程，第5卷，聚合物结构，29（1995）