Joint study on the standardization of laboratory rats

在国际实验动物科学理事会(ICLAS)的支持下，国际大鼠遗传命名委员会(RGNC)重新成立，以激活委员会的活动，第一次和第二次委员会会议分别于1994年在日本札幌和1996年在法国图卢兹举行。所取得的成果概述如下：(1)设立了五个小组委员会，以帮助和发展委员会的活动。近亲繁殖的品系。聚合酶链式反应监测老鼠基因组学：基因符号，DNA符号，染色体。MHC遗传学：基因、符号、重组。大鼠基因组(2)近交系和近交系的命名规则、基因座、主要组织相容性复合体(MHc)区的命名规则进行了讨论和执行。(3)聚合酶链式反应监测系统是聚合酶链式反应监测委员会新开发的遗传监测系统。(4)该委员会的官方期刊由《大鼠通讯》改为《大鼠基因组》，并从1994.2起连续出版6卷，为期3年。利用互联网建立信息交流系统，实现委员之间的顺畅沟通。对大鼠冷冻保存技术进行了研究和开发。将匹兹堡大学医学院保存的大鼠品系作为免疫遗传学和移植研究的国际参考品系引入中央实验动物研究所并保存在ICLAS胚胎库中。对匹兹堡大学医学院保存的标准菌株进行微生物清洗。

International Rat Genetic Nomenclature Committee (RGNC) were re-established under the auspicies of the ICLAS (International Council for Laboratory Animals Science) to activate the committee activity and the 1st and 2nd committee meetings were held in Sapporo (Japan) in 1994 and in Toulouse (France) in 1996, respectively. The results obtained are summarized as follows.(1) Five sub-committees were established to help and develop the committee's activities.a. Inbred strainsb. PCR monitoringc. Rat genomics : gene symbols, DNA symbols, chromosomesd. MHC genetics : genes, symbols, recombinantse. Rat Genome(2) Rules of nomenclature of inbred strains and outbred stocks, gene loci, MHC (major histocompatibility complex) region were discussed and enforced.(3) PCR monitoring system was newly developed for genetic monitoring by the PCR monitoring committee.(4) The official journal of the committee were changed from Rat News Letter to Rat Genome and six volumes were published for three years from 1994.2. Information exchange system was established using the Internet of communicate smoothly among committee members.3. Research and development of cryopreservation techniques for the rat were carried out. Rat strains maintained in University of Pittsburgh School of Medicine as the international reference strains for immunogenetics and transplantation studies were introduced to the Central Institute for Experimental Animals and preserved in the ICLAS Embryo Bank.4. Microbiological cleaning of the reference strains maintained in University of Pittsburgh School of Medicine was carried out.