Joint Research on Pathogenesis of Exfoliation Syndrome

剥脱综合征发病机制联合研究

基本信息

批准号：
06044265
负责人：
INOMATA Hajime
金额：
--
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Survey.
财政年份：
1994
资助国家：
日本
起止时间：
1994 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044265/
关键词：
Exfoliation syndrome Exfoliation glaucoma Exfoliative material Anterior chamber angle Trabecular meshwork Glaucoma Pseudoexfoliation of the lens capsule Pigmentary glaucoma

项目摘要

1. Clinical Study on Pathogenesis of Exfoliation SyndromeThe anterior chamber pigment dispersions after pupillary dilatation, anterior lens surface pigment particle, and angle pigmentation were significantly evident in Japanese patients with exfoliation syndrome than the normal control. No evident differences were detected in clinical signs between the exfoliation eyes with and without glaucoma.2. Pathological study on Pathogenesis of Exfoliation SyndromeExfoliation materials were observed along the epithelial cells of the iris and ciliary body, and in the trabecular meshwork and zonules. The trabecular meshwork showed increase in the thickness of the trabecular beams and deposition of pigment granules in the trabecular cells. It was concluded that exfoliation glaucoma was a kind of pigmentary glaucoma causing degeneration of trabecular cells and consequent thickening of the trabecular sheets. In tissue specimens treated with cupromeronic blue, electron-dense filaments were seen associated with the exfoliation materials. In the studies with enzymatic digestion technique, it was demonstrated that exfoliation materials contain chondroitin sulfate, dermatan sulfate, heparan sulfate proteoglycans.3. Molecular-Genetic Study on Pathogenesis of Exfoliation SyndromeThirty-four patients seen in Kyushu University Eye Clinic and 317 healthy controls were studied. HLA class I antigens were determined by serological typing using the NIH standard micro-cytotoxicity method with highly selected antisera. DNA typing of HLA class II alleles were performed using the technique with hybridization of polymerase chain reaction-amplified DNA with sequence-specific oligonucleotide probes. The frequency of DRB1^*0405 and DQA1^*03 showed lower tendency in the patients compared with controls, but no statistical significance. The findings suggest that there may be the genetic predisposition to exfoliation syndrome.

1。毕生扩张，前晶状体表面色素颗粒和角色素沉着的骨化综合综合症发病机理的临床研究在日本剥落综合征患者中比正常对照的患者显而易见。在有和没有青光眼的剥落眼睛之间，没有发现明显的差异。2。沿着虹膜和纤毛体的上皮细胞以及小梁网状和Zonules观察到了去角质综合脱糖材料发病机理的病理研究。小梁网状作品显示小梁束的厚度增加以及小梁细胞中色素颗粒的沉积。得出的结论是，去角质青光眼是一种色素青光眼，导致小梁细胞变性，因此小梁片的增厚。在用库米蓝色处理的组织标本中，可以看到电子致密细丝与去角质材料相关。在使用酶消化技术的研究中，证明去角质材料含有硫酸软骨素，硫酸皮肤菌，硫酸乙酰肝素蛋白聚糖3。研究了在京都大学眼科诊所和317个健康对照的剥落综合综合症患者的发病机理的分子遗传学研究。 HLA I类抗原通过使用具有高度选择的抗血清的NIH标准微毒性法确定血清学分类。使用该技术与序列特异性寡核苷酸探针杂交的技术进行了HLA II类等位基因的DNA键入。与对照组相比，DRB1^*0405和DQA1^*03的频率显示出患者的趋势较低，但没有统计学意义。研究结果表明，可能存在去角质综合征的遗传倾向。