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Biochomistry and Food Chamistry of Exracellular matrix components of fish and Shell-fish

鱼和贝类细胞外基质成分的生物化学和食物化学

基本信息

批准号：
06303013
负责人：
SAKAGUCHI Morihiko
金额：
$ 17.09万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

In the present study, we identified the components present in the extracellular matrix of fish and marine vertebrates and examined their structure and properties. We also studied the enzymes involved in the breakdown of collagen, a major matrix component, in association with post-mortem change in the muscle texture. Major results obtained are as follows : (1) Molecularspecies of sea urchin and sea cucumber collagens are composed of (alpha1) _2alpha2. (2) Composition of fish proteoglycan is studied, and mitogenic activities of liver and bone in rat tested by the oral administration were detected. (3) Fibronectins were purified from various fish serum and the conditioned medium of carp fibroblast cells. By comparing the biological activity, the difference in the cell spreading activity was demonstrated between serum and cellular fibronectins. (4) Subunit composition of type V collagen of rainbow trout muscle was clarified and the degradation of high molecular substance of type V collagen during ice-storage was demonstrated. (5) Collagenolytic activity was not detected in serum, but various gelatinolytic activities derived from serine-type proteinases (70kDa-120kDa) and metallo-type proteinases (<100kDa) were detected in the extracts of various fish tissues. (6) Tenderization of muscle tissue possibly due to the solubilization of skin collagen was demonstrated for common squid. (7) Subunit composition of kuruma prawn and Antarctic krill was studied and disintegration of pericellular connective tissues of kuruma prawn during chill-storage was shown to be partly due to the biochemical change of alpha2 (AR-I) component. (8) Gelationlytic activity derived from a 148kDa factor most active in acidic pH range was detected in abalone muscle and the N-terminal amino acid sequence was determined.

在本研究中，我们确定了存在于鱼类和海洋脊椎动物的细胞外基质中的组分，并研究了它们的结构和性质。我们还研究了参与胶原蛋白分解的酶，胶原蛋白是一种主要的基质成分，与死后肌肉质地的变化有关。主要结果如下：（1）海胆和海参胶原蛋白的分子组成均为（α 1）_2 α_2。(2)研究了鱼肉蛋白多糖的组成，并测定了鱼肉蛋白多糖对大鼠肝脏和骨骼的促有丝分裂活性。(3)从各种鱼血清和鲤鱼成纤维细胞条件培养液中纯化纤维连接蛋白。通过比较生物活性，证实了血清和细胞纤连蛋白在细胞铺展活性上的差异。(4)阐明了虹鳟鱼肌肉中V型胶原蛋白的亚基组成，以及V型胶原蛋白中高分子物质在冰藏过程中的降解。(5)在血清中未检测到胶原分解活性，但在各种鱼组织提取物中检测到来源于丝氨酸型蛋白酶（70 kDa-120 kDa）和金属型蛋白酶（<100 kDa）的各种明胶分解活性。(6)鱿鱼肌肉组织的嫩化可能是由于皮肤胶原蛋白的溶解。(7)对日本对虾和南极磷虾的亚基组成进行了研究，结果表明，日本对虾在冷藏过程中细胞周结缔组织的解体部分是由于α 2（AR-Ⅰ）组分的生化变化。(8)在鲍鱼肌肉中检测到一个分子量为148 kDa的在酸性pH范围内最具活性的凝胶溶解因子，并测定了其N端氨基酸序列。