Studies on the Regulatory Genes of Gonadal Differentiation from the Viewpoint of Utilization in Animal Resource Science.

从动物资源科学利用的角度研究性腺分化的调控基因。

• 批准号: 06304021
• 负责人: TACHI Chikashi
• 金额: $ 9.73万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06304021/
• 关键词: SRY; c-kit; P450Aromatase; Sertoli cells; Sex determination; apoptosis; ovary; transgenic animals; cyclin; Sry; WAP; fos; P450 Aromatase; 黄体

Representative results obtained during the tenure of the grant are as follows. 1) Coplete genomic SRY (sex determining region on Y chromosome) DNA of Shiba goat (Capra hircus var Shiba) spanning 4.9 kb was cloned and sequenced. 2) Transformed cell lines were established by transfecting TM-4 cells derived from mouse Sertoli cells, with human and mouse SRY DNA.Curiously, as it turned out, the expression of P450aromatese genes was induced by the transfected mouse SRY DNA.This cell line is currently investigated in order to identify the genes which constitute the putative SRY cascade. 3) Bovine SRY cDNA isolated from a fetal testicular cDNA library is confirmed to possess circular conformation. 4) Caprine c-kit cDNA was cloned and sequenced. It turned out that the intracellular domain of caprine c-kit possess one extra alanine insert which have not been described in any other species except sheep so far examined. The insert appears to be limited to goats and sheep. Attempts are being made … More to determine the biological meaning of the insert. 5) Interesting mutant genes were identified in human patients suffering from the disorders of sexual differetiation. Investigations were made to clarify the function of the gene located on Y chromosome and putatively promote the growth of the body. 6) Detailed analysis of phenotypes of W^f and W^<sh> mouse mutants revealed several interesting features which may be useful to approach the understanding of functioning of c-kit/Sl system in ruminants. 7) Novel genes which might be responsible for the apoptosis of lutcal cells in the rat ovary, were identified. 8) Functions of oncognenes in apoptotic changes in the follicles have been investigated. 9) Role of cyclins in maturation division of oocytes has been investigated. 10) Tissue specificity of the WAP promoter was analyzed, and found to be a 'lcaky' promoter functioning in many tissues other than the mammary gland. 11) Attempts were made to improve efficiency of the production of transgenic animals by selecting embryos which successfully incorporated the transgenes into their genome by combined treatment with restrictions cnzymes and PCR analysis. Less

在赠款期间获得的代表性结果如下。 1）克隆并测序了4.9 kb的shiba山羊（Capra hircus var shiba）的复杂基因组SRY（Y染色体上的性别确定区域）DNA（Capra Hircus var shiba）。 2）通过转染源自小鼠sertoli细胞的TM-4细胞（用人和小鼠的DNA）建立了转化的细胞系。事实证明，P450Aromatese基因的表达是由翻译后的小鼠SRY DNA诱导的。目前研究了该细胞系，以识别该基因构成了推测的casscade casscade。 3）从胎儿检测cDNA文库中分离出的牛sry cDNA被证实是潜在的圆形组成。 4）克隆并测序了辣椒丝C-kit cDNA。事实证明，Caprine c-kit位置的细胞内结构域是一个额外的丙氨酸插入物，除了迄今为止检查的绵羊以外的任何其他物种中尚未描述过。插入物似乎仅限于山羊和绵羊。正在尝试……更多的是确定插入物的生物学意义。 5）在患有性别分化疾病的人类患者中发现了有趣的突变基因。进行了研究，以阐明位于Y染色体上的基因的功能，并推测促进人体的生长。 6）对W^f和w^<sh>小鼠突变体的表型的详细分析揭示了一些有趣的特征，这些特征可能有助于理解反刍动物中c-kit/sl系统的功能。 7）鉴定出可能导致大鼠卵巢中Lutcal细胞凋亡的新型基因。 8）已经研究了o骨知识在凋亡变化中的功能。 9）细胞周期蛋白在卵母细胞成熟分裂中的作用已被研究。 10）分析了WAP启动子的组织特异性，并发现在乳腺以外的许多组织中起作用是“ LCAKY”启动子。 11）尝试通过选择胚胎成功地将翻译纳入其基因组中的胚胎和限制cnzymes和PCR分析，从而提高转基因动物的产生效率。较少的

项目成果

Nakagawa Y,H.Takeuchi et al.: "Restriction fragment length polymorphisms of the CYP11B1 gene in the ........" Jap.J.Human Genet.38. 203-207 (1993)

Nakakawa Y,H.Takeuchi 等人：“……中 CYP11B1 基因的限制性片段长度多态性”Jap.J.Human Genet.38。

Tsutsumi O.,Iida T,et al.: "Intact sex determining region Y (SRY) in a patient with XY pure gonadal dysgenesis and a twin brother." Endocrine Journal. 41. 281-285 (1994)

Tsutsumi O.、Iida T 等人：“XY 纯性腺发育不全患者和双胞胎兄弟的完整性别决定区 Y (SRY)。”

R-Z Lu, et al.: "Activin A and superoxide dismutase syner-gistically enhance development of 1-cell mouse embryos to the blastocyst stage in vitro" J.Reprod.Dev.40. 33-38 (1994)

R-Z Lu 等人：“激活素 A 和超氧化物歧化酶协同促进 1 细胞小鼠胚胎在体外发育至囊胚阶段”J.Reprod.Dev.40。

O.Tsutsumi, et al.: "Intact sex determining region (SRY) in a patient with XY pure gonadal dysgenesis and a twin brother." Endocr.J.41. 281-285 (1994)

O.Tsutsumi 等人：“XY 纯性腺发育不全患者和双胞胎兄弟的完整性别决定区 (SRY)”。

T. Hara, C. Tachi: "Expression of matrix metalloproteinase-11 gene in..." J. Reproduction and Development. 41. 287-292 (1995)

T. Hara、C. Tachi：“基质金属蛋白酶 11 基因在……中的表达”J. 繁殖与发育。