Attempts to Identify Monokine-Like Substances Which Induce Cell Proliferation in the Ectoplacental Cone of Mouse Embryos

尝试鉴定诱导小鼠胚胎外胎盘锥细胞增殖的单核因子样物质

基本信息

批准号：
03804062
负责人：
TACHI Chikashi
金额：
$ 1.15万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

We proposed on the basis of our own observations in rats and mice that macrophages may be functionally involved in the embryomaternal interactions during the peri-nidatory period in eutherian mammals (Tachi et al., 1981, 1986). Recently, much interest has been focused upon the possible role played by the phagocytes during early gestation. We improved method for the culture of macrophages in vitro (Tachi, 1988), and, at the same time, developed a new method for culture of mouse blastocysts in vitro suitable for quantitative analysis (Tachi, 1991, 1992). Using these methods we found that the macrophage-conditioned medium stimulate highly the proliferation of cells in the ectoplacental cone (EPC in the following) of the cultured blastocysts (Tachi, 1991). The effective substance(s) contained in the macrophage-conditioned medium is probably below 30,000 in molecular weight, and differ from EGF or FGF. It was reported that IGF-1 stimulates the proliferation of EPC cells of the placenta isolated from mice of 10.5 Day of pregnancy (Kanai-Azuma, 1992). However, the effects of IGF-1 on the EPC cells of the cultured blastocysts are at present not so conspicuous. While the present research projects were in progress, GM-CSF (Athanassakis et al., 1991) and IL-3 (Mori et al., 1992) were reported to stimulate the EPC cell proliferation. Furthermore, M-CSF were found to play important roles in implantation of mouse embryos, by mediating certain crucial processes in the endometrium (Pollard et al., 1992). Since IL-3, GM-CSF and M- CSF are well know monokines, it may be said that the importance of the monokines in the implantation of muridae rodents are being gradually elucidated in the literature. Although it was not possible to finally identify the molecular nature of the substance(s) in the macrophage-conditioned medium, the research efforts are still continued in our laboratory.

我们根据自己在大鼠和小鼠中的观察结果提出，巨噬细胞可能在纽约时期的脑哺乳动物期间可能与胚胎的相互作用有关（Tachi等，1981，1986）。最近，人们非常关注吞噬细胞在早期妊娠期间所扮演的角色。我们改进了体外巨噬细胞培养的方法（Tachi，1988），同时，为小鼠胚泡培养的新方法提供了适合定量分析的新方法（Tachi，1991，1992）。使用这些方法，我们发现巨噬细胞条件的培养基高度刺激了培养的胚泡的外卵形锥（EPC）中细胞的增殖（Tachi，1991）。巨噬细胞条件培养基中包含的有效物质的分子量可能低于30,000，与EGF或FGF不同。据报道，IGF-1刺激了从10.5天的妊娠小鼠中分离出的胎盘的EPC细胞的增殖（Kanai-Azuma，1992）。但是，目前，IGF-1对培养的胚泡EPC细胞的影响并不那么明显。尽管目前的研究项目正在进行中，但据报道GM-CSF（Athanassakis等，1991）和IL-3（Mori等，1992）刺激EPC细胞增殖。此外，发现M-CSF通过介导子宫内膜中的某些关键过程，在小鼠胚胎的植入中起重要作用（Pollard等，1992）。由于IL-3，GM-CSF和M-CSF是众所周知的Monokines，因此可以说Monokines在文献中逐渐阐明了Monokines在植入Muridae啮齿动物中的重要性。尽管不可能最终确定巨噬细胞条件培养基中物质的分子性质，但我们的实验室仍在继续进行研究工作。