Studies on Regulation of Macrophages Function during Nidation-Molecular and Cell Biological Studies-)

着床过程中巨噬细胞功能调控的研究-分子与细胞生物学研究-)

• 批准号: 07044182
• 负责人: TACHI Chikashi
• 金额: $ 9.22万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044182/
• 关键词: Placenta; Implantation; Macrophages; Luminal epithelial cells; Trophoblast cells; Cell culture; Signal transduction; Cytokines; Transgenic animals; シバヤギ; 子宮上皮細胞; トランスジェニック; MMP; MAPキナーゼ

The in vitro manipulation of mammalian embryos, including the introduction of exogenous genes, will be considered to be complete, in many instances, only after the successful embryo transfer leading to the birth of newborns. It has been well recognized, however, that the enbryo transfer suffers from poor success rates, the implantation processes being the notorious bottle neck. In order to prevent the implantation failure, the elucidation of the mechanisms underlying the cellular or molecular processes of implantation will be essential. The main purpose of the present Israel-Japan bi-national co-operation projects is to acquire the Israeli expertise and information concerning the macrophage research and the production of transgenic goats.We have attempted to establish in vitro cultured cell lines of trophoblast cells, the luminal epithelial cells of the endometrium and macrophages of Shiba goats in order to work out the in vitro co-culture systems which will serve as a model to analyze … More the influence of macrophages upon the implantation and subsequent placetation processes. We could establish the cultured cell lines of caprine luminal epithelial cells, although they have not yet been cloned. The caprine luminal epithelial cells undergo formation of gland-like structures in vitro, and some of the cells were seen to differentiate into secretory cells possessing full of secretory granules in the cytoplasm. Experiments are underway to clone these epithelial cell lines.Trophoblast cell cultures suffered from the sudden reduction in the proliferative capacities, after passages of several passages. attempts are being made to restore the proliferative capacities of the putative trophoblast cell lines.Macrophages of Shiba goats turned out to be difficult to obtain either from the bone marrow or from the spleen. Owing to some unknown reasons, the caprine macrophages appear to behave differently from the murine cells. Further experiments are needed to establish methodology for the collection and culture of the caprine macrophages.On Israeli side, biochemical analysis of the signal transduction systems which operate during the activation of macrophages in currently in progress.Exchange of valuable up-to-date information concerning the experiments in not only macrophages but also production of transgenic goats, possible application of MRI methods in implantation reserach and in vitro maturation of murine oocytes, were done. Less

在许多情况下，只有在成功的胚胎移植导致新生儿出生之后，哺乳动物胚胎的体外操作，包括引入外源基因，才被认为是完整的。然而，众所周知，胚胎移植的成功率很低，植入过程是众所周知的瓶颈。为了防止植入失败，阐明植入的细胞或分子过程的机制至关重要。目前以色列-日本两国合作项目的主要目的是获取以色列在巨噬细胞研究和转基因山羊生产方面的专业知识和信息。我们尝试建立柴山羊滋养层细胞、子宫内膜上皮细胞和巨噬细胞的体外培养细胞系，以制定体外共培养系统，作为模型。 分析巨噬细胞对植入和随后的放置过程的影响。我们可以建立山羊管腔上皮细胞的培养细胞系，尽管它们尚未被克隆。山羊管腔上皮细胞在体外形成腺样结构，部分细胞分化为胞浆内充满分泌颗粒的分泌细胞。克隆这些上皮细胞系的实验正在进行中。滋养层细胞培养物在传代数次后，增殖能力突然下降。人们正在尝试恢复假定的滋养层细胞系的增殖能力。事实证明，无论是从骨髓还是从脾脏中获取柴山羊的巨噬细胞都是困难的。由于一些未知的原因，山羊巨噬细胞的行为似乎与小鼠细胞不同。需要进一步的实验来建立山羊巨噬细胞的收集和培养方法。以色列方面，目前正在进行巨噬细胞激活过程中运行的信号转导系统的生化分析。交换有关巨噬细胞实验和转基因山羊生产、MRI方法在植入研究和体外成熟中的可能应用的有价值的最新信息 小鼠卵母细胞，完成。较少的

项目成果

Suenaga A: "Quantitative analysis of the spreading of the mouse trophoblast in vitro:a model for early invasion." Placenta. 17. 583-590 (1996)

Suenaga A：“体外小鼠滋养层扩散的定量分析：早期入侵模型。”

Y.Kawamata,C.Tachi,H.Tojo: "Quantitative analysis of the ex-pression of whey acidic protein." J.Reproduction and Development. 41. 331-338 (1995)

Y.Kawamata，C.Tachi，H.Tojo：“乳清酸性蛋白表达的定量分析。”

J.Wen,H.Tojo,C.Tachi: "Expression of whey acidic protein (WAP) gene in tissues other than.." Molecular Reproduction and Development. 41. 339-406 (1995)

J.Wen，H.Tojo，C.Tachi：“乳清酸性蛋白（WAP）基因在除..以外的组织中的表达”分子复制和发育。

Ilan M: "β-lactoglubulin/human serum albumin fusion genes do not respond accurately to signals from extracellular matrix in mammary epithelial cells from transgenic mice." Experimental Cell Research. 228. 146-159 (1996)

Ilan M：“β-乳球蛋白/人血清白蛋白融合基因不能准确响应转基因小鼠乳腺上皮细胞中的信号。” 228. 146-159 (1996)。

Ilan M: "Dual regulation of beta-lactoglubulin/human serum albumin gene expression by the extracellular matrix in mammary cells from transgenic mice." Experimental Cell Research. 224. 28-38 (1996)

Ilan M：“转基因小鼠乳腺细胞中细胞外基质对 β-乳球蛋白/人血清白蛋白基因表达的双重调节。”