Studies on Regulation of Macrophages Function during Nidation-Molecular and Cell Biological Studies-)

哺乳动物胚胎的体外操作，包括外源基因的引入，在许多情况下，只有在胚胎移植成功导致新生儿出生后才被认为是完整的。然而，众所周知，胚胎移植的成功率很低，植入过程是臭名昭著的瓶颈。为了防止植入失败，阐明植入的细胞或分子过程的机制将是必不可少的。目前以色列-日本两国合作项目的主要目的是获得以色列关于巨噬细胞研究和转基因山羊生产的专门知识和信息。我们尝试建立滋养细胞、子宫内膜腔上皮细胞和巨噬细胞的体外培养细胞系，建立巨噬细胞体外共培养体系，并以此为模型分析巨噬细胞对植入及其后续安置过程的影响。我们可以建立山羊腔上皮细胞的培养细胞系，但尚未克隆。羊腔上皮细胞在体外形成腺样结构，部分细胞分化为分泌细胞，胞浆内充满分泌颗粒。克隆这些上皮细胞系的实验正在进行中。滋养细胞培养在经过几代传代后，增殖能力突然下降。正在尝试恢复假定的滋养细胞系的增殖能力。结果表明，柴山羊的巨噬细胞无论是从骨髓还是从脾脏都很难获得。由于一些未知的原因，山羊巨噬细胞的行为似乎与小鼠细胞不同。需要进一步的实验来建立山羊巨噬细胞的收集和培养方法。在以色列方面，巨噬细胞活化过程中信号转导系统的生化分析目前正在进行中。就巨噬细胞实验、转基因山羊的生产、MRI方法在植入研究和小鼠卵母细胞体外成熟方面的可能应用等方面进行了有价值的最新信息交流。少

The in vitro manipulation of mammalian embryos, including the introduction of exogenous genes, will be considered to be complete, in many instances, only after the successful embryo transfer leading to the birth of newborns. It has been well recognized, however, that the enbryo transfer suffers from poor success rates, the implantation processes being the notorious bottle neck. In order to prevent the implantation failure, the elucidation of the mechanisms underlying the cellular or molecular processes of implantation will be essential. The main purpose of the present Israel-Japan bi-national co-operation projects is to acquire the Israeli expertise and information concerning the macrophage research and the production of transgenic goats.We have attempted to establish in vitro cultured cell lines of trophoblast cells, the luminal epithelial cells of the endometrium and macrophages of Shiba goats in order to work out the in vitro co-culture systems which will serve as a model to analyze … More the influence of macrophages upon the implantation and subsequent placetation processes. We could establish the cultured cell lines of caprine luminal epithelial cells, although they have not yet been cloned. The caprine luminal epithelial cells undergo formation of gland-like structures in vitro, and some of the cells were seen to differentiate into secretory cells possessing full of secretory granules in the cytoplasm. Experiments are underway to clone these epithelial cell lines.Trophoblast cell cultures suffered from the sudden reduction in the proliferative capacities, after passages of several passages. attempts are being made to restore the proliferative capacities of the putative trophoblast cell lines.Macrophages of Shiba goats turned out to be difficult to obtain either from the bone marrow or from the spleen. Owing to some unknown reasons, the caprine macrophages appear to behave differently from the murine cells. Further experiments are needed to establish methodology for the collection and culture of the caprine macrophages.On Israeli side, biochemical analysis of the signal transduction systems which operate during the activation of macrophages in currently in progress.Exchange of valuable up-to-date information concerning the experiments in not only macrophages but also production of transgenic goats, possible application of MRI methods in implantation reserach and in vitro maturation of murine oocytes, were done. Less