ESTABLISHMENT OF DNA DIAGNOSES IN VETERINARY MEDICINE

建立多种兽医学DNA诊断体系，开展遗传病、传染病、肿瘤、动物MHC_s的诊断研究。遗传性疾病：在牛白细胞黏附缺乏症中，发现cd18分子的128Asp被Gly取代。在牛凝血因子XIII缺乏症中，FXIIIA基因的83Phe被Ser取代。为这两种遗传性疾病开发了PCR诊断系统。传染病：PCR检测系统可用于多种动物的鹦鹉热衣原体和伯纳克希菌。在猫免疫缺陷病毒感染中，除建立PCR检测系统外，还可建立FIV亚型鉴定系统。建立了牛免疫缺陷病毒的PCR检测系统。肿瘤：利用t细胞受体和免疫球蛋白基因探针进行Southern blot分析，建立了用于淋巴肿瘤细胞谱系和克隆性鉴定的DNA诊断系统。犬p53基因的PCR-SSCP分析表明，p53突变存在于多种肿瘤中，包括淋巴瘤、白血病、骨肉瘤和结肠癌。对癌基因和逆转录病毒共同整合位点的检查显示，在11例自然发生的淋巴瘤中，有5例存在病毒诱导的异常，c-myc异常与其他癌基因异常存在相关性。动物MHC_s：从犬B淋巴母细胞细胞系中分离出两种MHC DRB克隆，并利用这些序列进行MHC分型。

To establish various DNA diagnosis systems in the veterinary medicine, we carried out diagnostic researches on hereditary diseases, infectious diseases, tumors and animal MHC_s.Hereditary diseases : In bovine leukocyte adhesion deficiency, 128Asp of CD 18 molecule was found to be substituted by Gly. In bovine blood coagulation factor XIII deficiency, 83Phe of FXIIIA gene was shown to be substituted by Ser. PCR diagnosis systems were developed for both of these hereditary diseases.Infectious diseases : PCR detection systems could be developed for Chlamydia psittaci and Coxiella burnetii in various animal species. In feline immunodeficiency virus infectoin, in addition to the establishment of PCR detection sysytem, identification system for FIV subtypes could be developed. PCR detection system for bovine immunodeficiency virus was also established.Tumors : DNA diagnosis system to identify lineage and clonarity of the lymphoid tumor cells was developed by using Southern blot analysis with T-cell receptor and immunoglobulin gene probes. PCR-SSCP analysis of canine p53 gene demonstrated the presence of p53 mutaions in a variety of tumors including lymphoma, leukemia, osteosarcoma and colon cancer. Examination of oncogenes and retroviral common integration sites revealed viral-induced abnormalities in 5 of 11 naturally occurring lymphomas and a correlation of c-myc abnormality with abnomality of other oncogenes.Animal MHC_s : Two kinds of MHC DRB clones were isolated from a canine B lymphoblastois cell line, and MHC typing became available by using these sequences.