Basic and Clinical Studies on abnormal expression of cytokine receptors in animals
Basic and Clinical Studies on abnormal expression of cytokine receptors in animals
批准号:
02454107
负责人:
HASEGAWA Atsuhiko
金额:
$4.22万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1990
资助国家:
日本
项目状态:
已结题
起止时间:
1990 至 1991
中文摘要
1.猫网状内皮细胞增生症是一种非常原始的猫骨髓增生性疾病,其获得的白血病细胞结构性表达白细胞介素2受体α链亚单位。将猫传染性腹膜炎病毒(FIPV)接种于培养的CRFK细胞,诱导其产生IL-6。患有FIP的猫的腹水中含有大量的IL-1。此外,还检测了IL-1α和βmRNA探针、转录产物在腹膜渗出液细胞中的表达以及β基因探针的表达。通过与生物素标记的人IL-1αcDNA探针的原位杂交,也证实了FIP猫炎性病变中巨噬细胞/单核细胞中IL-1αmRNA的表达。给予抗小鼠IL-6受体抗体可抑制IL-6诱导的血小板数量增加和特异性抗体应答。牛B类淋巴母细胞系BL2M3和BL312细胞释放促生长因子,这些细胞对这些因子也有反应。抗肿瘤相关抗原表位抗体可明显抑制促生长因子诱导的BL2M3细胞的增殖,并分析了细胞因子的产生及其受体的表达与家畜肿瘤和感染的关系。
英文摘要
1. The leukemic cells obtained from a cat with reticuloendotheliosis, which is a very, primitive type of feline myeloproliferative diseases, constitutively expressed interleukin (IL)-2 receptor alpha-chain subunit.2. The inoculation of feline infectious peritonitis (FIP) virus (FIPV) to cultured CRFK cells induced IL-6 production. Ascites from cats with FIP contained significant amounts of IL-1. Furthermore, IL-1 alpha and beta mRNA probes, the expression of franscripts were detected in peritoneal exudate cells and beta cDNA probes. By in situ hybridization with biotinylated human IL-1 alpha cDNA probes, the expression of IL-1 alpha mRNA was also confirmed in macrophage/monocytes infiltrated in the inflammatory lesions of FIP cats.3. Administration of anti-murine IL-6 receptor antibody inhibited the IL-6-induced increase of platelet numbers and specific antibody responses.4. The bovine B, Iymphoblastoid cell lines, BL2M3 and BL312 cells, released growth-promoting factors, and these cells also responded to the factors. The growth promoting factor-induced proliferation of BL2M3cells clearly i"nhibited by the addition of the antibody against one of the epitopes of tumor-associated antigen.The production of cytokines and the expression of their receptors were analyzed in association with the pathogenesis of neoplasms and infections in domestic animals.
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通讯作者:
Goitsuka,R.: "Characterization of a feline infectious peritonitis virus isolatc." J. Vet. Med. Sci.53. 337-339 (1991)
Goitsuka,R.:“猫传染性腹膜炎病毒分离株的特征。”
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Hiroshi Suzuki et al.: "Anti-murine IL-6 receptor antibody inhibits IL-6 effects in vivo" Immunol. Letters. 30. 17-22 (1991)
Hiroshi Suzuki 等人:“抗鼠 IL-6 受体抗体抑制体内 IL-6 效应”Immunol。
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共 22 条
HTLV-1-specific T cell suppression in infected individuals
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.24万
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财政年份:2011
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负责人:HASEGAWA Atsuhiko
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Effectiveness of small interfering RNA(siRNA)against the Mcl-1 gene in a canine mammary gland tumor cell line
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财政年份:2006
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Molecular Diagnosis of Mycoses
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批准号:12460141
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资助金额:$4.93万
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财政年份:2000
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ESTABLISHMENT OF DNA DIAGNOSES IN VETERINARY MEDICINE
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资助金额:$3.71万
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财政年份:1994
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负责人:HASEGAWA Atsuhiko
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Studies on receptors of animal lentiviruses
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批准号:05454123
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资助金额:$4.74万
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财政年份:1993
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负责人:HASEGAWA Atsuhiko
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Basic and clinical studies on the immunopathogenesis of viral infection
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批准号:63480093
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财政年份:1988
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负责人:HASEGAWA Atsuhiko
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依托单位:
海外基金