Basic and Clinical Studies on abnormal expression of cytokine receptors in animals

1.猫网状内皮细胞增生症是一种非常原始的猫骨髓增生性疾病，其获得的白血病细胞结构性表达白细胞介素2受体α链亚单位。将猫传染性腹膜炎病毒(FIPV)接种于培养的CRFK细胞，诱导其产生IL-6。患有FIP的猫的腹水中含有大量的IL-1。此外，还检测了IL-1α和βmRNA探针、转录产物在腹膜渗出液细胞中的表达以及β基因探针的表达。通过与生物素标记的人IL-1αcDNA探针的原位杂交，也证实了FIP猫炎性病变中巨噬细胞/单核细胞中IL-1αmRNA的表达。给予抗小鼠IL-6受体抗体可抑制IL-6诱导的血小板数量增加和特异性抗体应答。牛B类淋巴母细胞系BL2M3和BL312细胞释放促生长因子，这些细胞对这些因子也有反应。抗肿瘤相关抗原表位抗体可明显抑制促生长因子诱导的BL2M3细胞的增殖，并分析了细胞因子的产生及其受体的表达与家畜肿瘤和感染的关系。

1. The leukemic cells obtained from a cat with reticuloendotheliosis, which is a very, primitive type of feline myeloproliferative diseases, constitutively expressed interleukin (IL)-2 receptor alpha-chain subunit.2. The inoculation of feline infectious peritonitis (FIP) virus (FIPV) to cultured CRFK cells induced IL-6 production. Ascites from cats with FIP contained significant amounts of IL-1. Furthermore, IL-1 alpha and beta mRNA probes, the expression of franscripts were detected in peritoneal exudate cells and beta cDNA probes. By in situ hybridization with biotinylated human IL-1 alpha cDNA probes, the expression of IL-1 alpha mRNA was also confirmed in macrophage/monocytes infiltrated in the inflammatory lesions of FIP cats.3. Administration of anti-murine IL-6 receptor antibody inhibited the IL-6-induced increase of platelet numbers and specific antibody responses.4. The bovine B, Iymphoblastoid cell lines, BL2M3 and BL312 cells, released growth-promoting factors, and these cells also responded to the factors. The growth promoting factor-induced proliferation of BL2M3cells clearly i"nhibited by the addition of the antibody against one of the epitopes of tumor-associated antigen.The production of cytokines and the expression of their receptors were analyzed in association with the pathogenesis of neoplasms and infections in domestic animals.