Development of a new sterilization method using ozonated water for the infected carious dentin, pulp, and root canal

臭氧是对抗细菌、真菌、原生动物和病毒的强力抗菌剂。在本项目中，我们在实验模型上检测了臭氧化水对感染根管的杀菌能力。暴露于臭氧水（0.5 mg/L） 10- 30s后，感染根管中的优势细菌之一粪肠球菌（Enterococcus faecalis）的活力显著下降。用臭氧水处理牛牙感染根管的实验模型，粪肠球菌的活菌数比蒸馏水处理减少了十分之一，臭氧水（4 mg/L）处理120 s后几乎未检出细菌。臭氧化水与聚维酮碘、苄索氯铵等口服消毒剂在抗菌活性和对人细胞系的细胞毒性方面无显著差异。臭氧水（4 mg/L）处理120 s后，对感染根管混合感染模型的抗菌效果非常高，甚至在牙本质小管的深部也有效果。综上所述，这些结果表明臭氧水在感染根管的消毒中是有用的。

Ozone is a strong antimicrobial agent against bacteria, fungi, protozoa and viruses. In this project, we examined the sterilization ability of ozonated water on a experimental model for the infected root canal.After exposure to ozonated water (0.5 mg/L) for 10-30 s, the viability of Enterococcus faecalis, one of the predominant bacteria in a infected root canal, significantly decreased. With ozonated water treatment on a experimental model for the infected root canal using bovine tooth, the number of living Enterococcus faecalis was decreased to one tens compared to that with distilled water, and almost no bacteria were detected after being treated with ozonated water (4 mg/L) for 120 s. There were no significant differences in antimicrobial activity and cytotoxicity to human cell line between ozonated water and oral disinfectants such as povidone iodine and benzethonium chloride. After ozonated water (4 mg/L) treatment for 120 s, antimicrobial effect against mixed infection model for the infected root canal was very high level, even in a deep area of the dentinal tubules.Taken together, these results suggest that ozonated water should be useful in the sterilization for infected root canal.