Development of a new sterilization method using ozonated water for the infected carious dentin, pulp, and root canal

开发一种使用臭氧水对受感染的龋齿牙本质、牙髓和根管进行消毒的新方法

• 批准号: 13672005
• 负责人: TERASHITA Masamichi
• 金额: $ 1.92万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672005/
• 关键词: Ozonated water; Antimicrobial activity; Infected root canal; Disinfectants; Cytotoxicity; Safety; Enterococcus faecalis

Ozone is a strong antimicrobial agent against bacteria, fungi, protozoa and viruses. In this project, we examined the sterilization ability of ozonated water on a experimental model for the infected root canal.After exposure to ozonated water (0.5 mg/L) for 10-30 s, the viability of Enterococcus faecalis, one of the predominant bacteria in a infected root canal, significantly decreased. With ozonated water treatment on a experimental model for the infected root canal using bovine tooth, the number of living Enterococcus faecalis was decreased to one tens compared to that with distilled water, and almost no bacteria were detected after being treated with ozonated water (4 mg/L) for 120 s. There were no significant differences in antimicrobial activity and cytotoxicity to human cell line between ozonated water and oral disinfectants such as povidone iodine and benzethonium chloride. After ozonated water (4 mg/L) treatment for 120 s, antimicrobial effect against mixed infection model for the infected root canal was very high level, even in a deep area of the dentinal tubules.Taken together, these results suggest that ozonated water should be useful in the sterilization for infected root canal.

臭氧是一种针对细菌、真菌、原生动物和病毒的强抗菌剂。在这个项目中，我们在受感染根管的实验模型上检查了臭氧水的杀菌能力。接触臭氧水（0.5 mg/L）10-30秒后，受感染根管中的主要细菌之一粪肠球菌的活力显着下降。用臭氧水处理牛牙感染根管实验模型，与蒸馏水相比，活的粪肠球菌数量减少到几十个，并且用臭氧水（4 mg/L）处理120 s后几乎检测不到细菌。臭氧水与聚维酮碘、苄索氯铵等口服消毒剂的抗菌活性和对人体细胞系的细胞毒性没有显着差异。臭氧水（4 mg/L）处理120 s后，对感染根管的混合感染模型的抗菌效果非常高，甚至在牙本质小管的深处也是如此。综上所述，这些结果表明臭氧水应该可用于感染根管的消毒。