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Production of new method on peritoncal-irrigation cytology by use of releasing method of carcinoembryonic antigen by phosphatidylinositol phospholipase C

磷脂酰肌醇磷脂酶C释放癌胚抗原法建立腹膜冲洗细胞学新方法

基本信息

批准号：
06454363
负责人：
KUNIEDA Katsuyuki
金额：
$ 3.65万
依托单位：
Gifu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454363/
关键词：
peritoneal dissemination irrigation cytology gastric cancer CEA phosphatidylinositol phospholipase C assay system PIPCL 診断 PIPLC

项目摘要

To get early diagnosis of peritoneal dissemination on gastric cancer, usefulness of a novel method using release of carcinoembryonic antigenA (CEA) from the cancer cells of peritoneal irrigation by Phosphatidylinositol phospholipase C (PI-PLC) was evaluated. Experimental study : 1) The CEA value was increased about 1.7 times by PI-PLC against KATO-III cancer cells. 2) The CEA value increased depend on duration of incubation with PI-PLC and number of target cells. Clinical study : Two kinds of irrigation cytology were performed at laparotomy on 81 gastric cancer patients, that is irrigation cytology (IR) and PI-PLC.1) Positive rate of 12 patients with macroscopic peritoneal dessemination (P) was 91% of IR and 83% of PI-PLC,while it was 2.9% and 7.2% respectively in 69 cases without P.2) CEA sensitibity showed from 1.8 times to 600 times, and characteristic showing positive PI-PLC was the cases suggesting microscopic peritoneal dissemination from clinico-pathological findings. It is sugg … More ested that PI-PLC was a useful method on objective detection for early peritoneal dissemination. By use of PI-PLC,we newly produced a novel kits for peritoneal irrigation cytology. Method : The peritoneal sediment obtained from peritoneal irigation and filtrated by glass microfiber filter were **xed on the plate coated with anti-CEA antibody and added 0.05 U of PIPLC.The positive reaction was given if there was color change between the plate with PIPLC and without it after incubation for 60 minutes and colored by Sandwitch method (PIPLC-kit). The comparison was performed among PIPLC-kit and IR using 53 gastric cancer patients. Results : The 16 patients showing positive reaction by IR were also all positive by PIPLC-kit. While the 37 patients showing negative by IR showed positive by PIPLC-kit. The 42 patients without macroscopic peritoneal metastases showed positive reaction in 5 cases by IR and 10 cases by PIPLC-kit. It is suggested that the PIPLC-kit was useful and simple assay system for the detection of early peritoneal dissemination. Less

为早期诊断胃癌腹膜转移，应用磷脂酰肌醇磷脂酶C（PI-PLC）从腹腔冲洗液中释放癌胚抗原A（CEA）的新方法。实验研究：1）PI-PLC对KATO-Ⅲ癌细胞的CEA值提高约1.7倍。2)CEA值的增加取决于与PI-PLC孵育的持续时间和靶细胞的数量。临床研究：对81例胃癌患者行腹腔冲洗细胞学（IR）和PI-PLC两种检查，结果显示：1）12例腹腔大体剥脱（P）患者，IR阳性率为91%，PI-PLC阳性率为83%;而69例无P者分别为2.9%和7.2%。2）CEA敏感性为1.8 ~ 600倍，特征性PI-PLC阳性者为临床病理提示腹膜镜下播散者。它是 ...更多信息结论：PI-PLC是一种较好诊断早期腹膜播散的方法。利用PI-PLC，我们新研制了一种新型的腹腔冲洗细胞学试剂盒。方法：取经玻璃微纤维过滤器过滤的腹腔沉淀物，固定于抗CEA抗体包被的平板上，加入0.05U的PIPLC，孵育60 min后，用Sandwitch法（PIPLC-kit）显色，以有或无PIPLC的平板颜色发生变化为阳性反应。对53例胃癌患者进行了PIPLC-kit和IR的比较。结果：16例IR阳性的患者，PIPLC-kit检测均为阳性。而37例IR阴性的患者，PIPLC-kit检测均为阳性。42例无肉眼腹膜转移的患者，IR阳性5例，PIPLC-kit阳性10例。提示该试剂盒是一种简便、实用的检测早期腹膜播散的方法。少