Production of new method on peritoncal-irrigation cytology by use of releasing method of carcinoembryonic antigen by phosphatidylinositol phospholipase C

为了早期诊断胃癌腹膜播散，我们评价了用磷脂酰肌醇磷脂酶C （PI-PLC）腹腔灌洗癌细胞释放癌胚抗原（CEA）的新方法的有效性。实验研究：1)PI-PLC对KATO-III型癌细胞的CEA值提高约1.7倍。2) CEA值随PI-PLC孵育时间和靶细胞数量的增加而增加。临床研究：对81例胃癌患者在开腹时进行两种冲洗细胞学检查，即冲洗细胞学（IR）和PI-PLC。1) 12例肉眼腹膜色散(P)患者IR阳性率为91%，PI-PLC阳性率为83%，无P者69例分别为2.9%和7.2%。2)CEA敏感性为1.8 ~ 600倍，临床病理表现为显微镜下腹膜播散者为PI-PLC阳性特征。进一步证明PI-PLC是早期腹膜播散客观检测的有效方法。利用PI-PLC，我们制作了一种新的腹膜冲洗细胞学试剂盒。方法：将腹膜冲洗所得的腹膜沉淀物经玻璃超细纤维过滤器过滤后，**于涂有抗cea抗体的平板上，加入0.05 U的PIPLC。用Sandwitch法（PIPLC-kit）染色，孵育60分钟后，加PIPLC与不加PIPLC的板有颜色变化，为阳性反应。对53例胃癌患者的PIPLC-kit与IR进行比较。结果：16例IR阳性患者，PIPLC-kit检测均为阳性。37例IR阴性，PIPLC-kit阳性。42例无肉眼腹膜转移的患者中，IR阳性5例，PIPLC-kit阳性10例。提示PIPLC-kit是一种检测早期腹膜播散的简便易行的检测系统。少

To get early diagnosis of peritoneal dissemination on gastric cancer, usefulness of a novel method using release of carcinoembryonic antigenA (CEA) from the cancer cells of peritoneal irrigation by Phosphatidylinositol phospholipase C (PI-PLC) was evaluated. Experimental study : 1) The CEA value was increased about 1.7 times by PI-PLC against KATO-III cancer cells. 2) The CEA value increased depend on duration of incubation with PI-PLC and number of target cells. Clinical study : Two kinds of irrigation cytology were performed at laparotomy on 81 gastric cancer patients, that is irrigation cytology (IR) and PI-PLC.1) Positive rate of 12 patients with macroscopic peritoneal dessemination (P) was 91% of IR and 83% of PI-PLC,while it was 2.9% and 7.2% respectively in 69 cases without P.2) CEA sensitibity showed from 1.8 times to 600 times, and characteristic showing positive PI-PLC was the cases suggesting microscopic peritoneal dissemination from clinico-pathological findings. It is sugg … More ested that PI-PLC was a useful method on objective detection for early peritoneal dissemination. By use of PI-PLC,we newly produced a novel kits for peritoneal irrigation cytology. Method : The peritoneal sediment obtained from peritoneal irigation and filtrated by glass microfiber filter were **xed on the plate coated with anti-CEA antibody and added 0.05 U of PIPLC.The positive reaction was given if there was color change between the plate with PIPLC and without it after incubation for 60 minutes and colored by Sandwitch method (PIPLC-kit). The comparison was performed among PIPLC-kit and IR using 53 gastric cancer patients. Results : The 16 patients showing positive reaction by IR were also all positive by PIPLC-kit. While the 37 patients showing negative by IR showed positive by PIPLC-kit. The 42 patients without macroscopic peritoneal metastases showed positive reaction in 5 cases by IR and 10 cases by PIPLC-kit. It is suggested that the PIPLC-kit was useful and simple assay system for the detection of early peritoneal dissemination. Less