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A NEWLY DEVELOPED METHOD EMPLOYING CEA RELEASING BY PHOSPHATIDYLINOSITOL PHOSPHOLOPASE C AND ULTRASOUND-INDUCED CELL LYSIS FOR EARLY DIAGNOSIS OF PERITONEAL DISSEMINATION

新开发的利用磷脂酰肌醇磷脂酶 C 释放 CEA 和超声诱导细胞裂解进行腹膜播散早期诊断的方法

基本信息

批准号：
09671219
负责人：
KUNIEDA Katsuyuki
金额：
$ 1.79万
依托单位：
GIFU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

[Purpose] Formerly, we devised novel method (PLC) employing the release of carcinoembryonic antigen (CEA) from cancer cells by phosphatidylinositol phospholipase C (PIPLC) for early diagnosis of peritoneal dissemination. This time, the PLC method was improved to obtain higher sensitivity for early detection of peritoneal dissemination by introducing ultrasound-induced cell lysis (USP method), and its usefulness was evaluated experimentally and clinically.[Materials] Three cell lines of CEA producing human gastric cancer (KATO-III, LoVo, MKN-45) and 196 patients with gastric cancer were the subjects of this study.[Methods] In the case of PLC, 0.05U of PIPLC was added in the sediment obtained from peritoneal lavaged fluid. In the cases of USP, a procedure of ultrasound-induced cell lysis was added to PLC Each positive rate was compared with that of conventional lavage cytology (CY).[Results] The effect of USP was better in KATO -III and MKN-45 with cytoplastic type than in LoVo with apical type. The positive rate of USP in the group with peritoneal dissemination was 94.1%, and was 0% in the group with T1. The positive rates of USP, PLC and CY were 15.3%, 9.5%, 5.4%,respectively with USP showing the highest rate. The prognosis of positive groups of USP, PLC and CY were significantly poorer than those of the negative groups in all cases. The prognosis of PLC positive group and PLC negative group was not significantly different, but that of USP positive group was significantly poorer than that of USP negative group.[Conclusion] From above results, USP is suggested as a more useful method for early diagnosis of peritoneal dissemination.

[目的]本研究利用磷脂酰肌醇磷脂酶C（PIPLC）释放癌胚抗原（CEA）的方法对腹膜转移进行早期诊断。这次，PLC方法进行了改进，以获得更高的灵敏度，通过引入超声诱导的细胞裂解（USP方法），早期检测腹膜传播，并评估其实用性的实验和临床。[材料]本研究以3株人胃癌CEA分泌细胞系（KATO-Ⅲ、LoVo、MKN-45）和196例胃癌患者为研究对象。[方法]以PLC为例，在腹腔灌洗液沉淀中加入0.05U的PIPLC。在美国药典中，将超声诱导细胞裂解程序加入到PLC中，并与常规灌洗细胞学（CY）的阳性率进行比较。[结果] USP对细胞质型KATO -Ⅲ和MKN-45的作用优于根尖型LoVo。腹膜播散组USP阳性率为94.1%，T1组为0%。USP、PLC、CY阳性率分别为15.3%、9.5%、5.4%，以USP阳性率最高。USP、PLC和CY阳性组的预后均明显差于阴性组。PLC阳性组与PLC阴性组的预后差异无统计学意义，USP阳性组的预后明显差于USP阴性组。[结论] USP是一种较好的早期诊断腹膜播散的方法。